[1]陈李影慧,吴武佳,秦东春#.PDTC对A549细胞增殖、细胞周期、凋亡及星形细胞上调基因1表达的影响[J].郑州大学学报(医学版),2013,(04):485.
 CHEN Liyinghui,WU Wujia,QIN Dongchun.Effect of PDTC on proliferation,cell cycle,apoptosis and AEG1 expression of A549 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):485.
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PDTC对A549细胞增殖、细胞周期、凋亡及星形细胞上调基因1表达的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年04期
页码:
485
栏目:
论著
出版日期:
2013-07-20

文章信息/Info

Title:
Effect of PDTC on proliferation,cell cycle,apoptosis and AEG1 expression of A549 cells
作者:
陈李影慧吴武佳秦东春#
郑州大学第一附属医院检验科 郑州 450052
Author(s):
CHEN Liyinghui WU Wujia QIN Dongchun
Clinical Laboratory, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
肺癌A549细胞PDTCNFκB凋亡星形细胞上调基因1
Keywords:
lung cancerA549 cellpyrrolidine dithiocarbamateNFκBapoptosisastrocyte elevated gene1
分类号:
R734.2
摘要:
目的:观察NFκB特异性抑制剂PDTC对人肺腺癌A549细胞增殖、细胞周期、凋亡及星形细胞上调基因1(AEG1)表达的影响。方法: 用不同浓度(25、50、100、200 μmol/L)的PDTC分别处理A549细胞,对照组不给PDTC。应用MTT法检测处理24、48、72 h后细胞增殖抑制率,流式细胞术检测处理24 h后细胞周期的变化,Hoechst33342荧光染色检测处理24 h后细胞的凋亡情况,RTPCR和Western blot检测处理24 h后细胞AEG1 mRNA和蛋白的表达。结果:经PDTC处理的A549细胞增殖明显受抑,呈时间和浓度依赖性(F时间=531.981,F浓度=388.475,P<0.05);与对照组相比,PDTC处理24 h后A549细胞G0/G1期细胞比例上升(P<0.05),细胞形态出现明显凋亡改变;随着PDTC浓度的增加,癌细胞内AEG1 mRNA和蛋白的表达水平降低(F=275.162、59.473,P<0.05)。结论:PDTC可抑制A549细胞的增殖,诱导其凋亡,其机制可能与PDTC抑制NFκB、AEG1的表达有关。
Abstract:
Aim: To explore the effect of pyrrolidine dithiocarbamate(PDTC) on proliferation,cell cycle,apoptosis of human lung cancer cell line A549 and expression of astrocyte elevated gene1(AEG1). Methods:A549 cells were treated by PDTC with different concentrations(25,50,100,200 μmol/L) for different time(24,48,72 h)respectively. MTT assay was used to detect the inhibition rates of cell growth. Flow cytometry was used to analyze the changes of cell cycle.Hoechst33342 staining was used to observe the apoptosis of cells with the fluorescence microscope. RTPCR and Western blot were used to detect the expression of AEG1. Results: PDTC inhibited the A549 cell proliferation in a time and dosedependent manner(Ftime=531.981,Fconcentration=388.475,P<0.05). In contrast to the control, the percentage of cells in G0/G1 phase increased significantly in PDTC group(P<0.05)with apparent apoptotic morphological changes. The mRNA and protein expressions of AEG1 were reduced with increasing concentration of PDTC(F=275.162 and 59.473,P<0.05). Conclusion: PDTC can inhibit the proliferation, and induce the apoptosis of A549 cells. The mechanism may be related to the downregulated expressions of NFκB and AEG1 gene induced by PDTC.

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备注/Memo

备注/Memo:
#通讯作者,男,1963年12月生,博士,教授,研究方向:肿瘤与分子免疫学,Email:qindongchun@zzu.edu.cn
更新日期/Last Update: 2013-07-25