[1]张芳,韩娜,蔡晶,等.BEZ235对A549细胞增殖及akt、mek、erk mRNA表达的影响[J].郑州大学学报(医学版),2013,(04):496.
 ZHANG Fang,HAN Na,CAI Jing,et al.Effects of BEZ235 on proliferation of A549 cells and mRNA expressions of akt, mek, and erk[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):496.
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BEZ235对A549细胞增殖及akt、mek、erk mRNA表达的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年04期
页码:
496
栏目:
论著
出版日期:
2013-07-20

文章信息/Info

Title:
Effects of BEZ235 on proliferation of A549 cells and mRNA expressions of akt, mek, and erk
作者:
张芳韩娜蔡晶方黎张中冕#
郑州大学第二附属医院肿瘤科 郑州 450014
Author(s):
ZHANG Fang HAN Na CAI Jing FANG Li ZHANG Zhongmian
Department of Oncology, the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014
关键词:
BEZ235肺肿瘤aktmekerkA549细胞
Keywords:
BEZ235lung neoplasmaktmekerkA549 cell
分类号:
R734.2
摘要:
目的:观察BEZ235对人肺腺癌A549细胞增殖及akt、mek、erk mRNA表达的影响。方法:体外培养A549细胞,分为对照组和BEZ235低、中、高剂量组,分别加入0、2.5、5、10 mol/L的BEZ235体外培养24、48、72 h;MTT法检测BEZ235对A549细胞增殖的抑制率,RTPCR技术检测处理48 h后akt、 erk、mek mRNA表达水平的变化。结果:BEZ235能够抑制A549细胞增殖,且呈浓度和时间依赖性(F浓度=233.315,F时间=171.598,F交互=6.770,P<0.001)。不同浓度BEZ235作用于A549细胞48 h后,akt mRNA的表达水平随BEZ235浓度的升高呈下降趋势(F=14.922,P<0.001),而mek、erk mRNA的表达水平无明显改变(F=2.968、3.059,P>0.05)。结论:BEZ235能够呈浓度和时间依赖性抑制A549细胞增殖,这种抑制作用可能与下调akt mRNA的表达有关。
Abstract:
Aim: To investigate the effect of BEZ235 on the proliferation of human lung cancer A549 cells and the expression levels of akt, mek, and erk mRNA. Methods: A549 cells were cultured in vitro with different concentrations BEZ235(0, 2.5, 5, 10 mol/L)for 24, 48, 72 h,respectively. The proliferation inhibition rate of A549 cells was assessed by MTT. After being treated for 48 h, the expressions of akt, mek, and erk mRNA were determined by RTPCR. Results: BEZ235 could inhibit the proliferation of A549 cells in vitro in a concentration and timedependent manner (Fconcentration=233.315,Ftime=171.598,Finteraction=6.770,P<0.001). After being treated by BEZ235 for 48 h, the mRNA expression level of akt decreased with the elevated concentration of BEZ235(F=14.922,P<0.001), while the mRNA expression levels of mek and erk had no significant change(F=2.968,3.059,P>0.05). Conclusion: BEZ235 can inhibit the proliferation of A549 cells in vitro in a dose and timedependent manner, which may relate to its suppressing the expression of akt mRNA.

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备注/Memo

备注/Memo:
#通讯作者,男,1964年12月生,硕士,主任医师,研究方向:恶性肿瘤的分子靶向治疗,Email:zhangzhongmian@sina.com
更新日期/Last Update: 2013-07-25