[1]张艳丽△,王秀芳,乔俊英,等.哮喘患儿外周血髓系抑制细胞、IL10和IL12水平检测*[J].郑州大学学报(医学版),2013,(04):502.
 ZHANG Yanli,WANG Xiufang,QIAO Junying,et al.Detection of MDSCs, IL10, and IL12 levels in peripheral blood from children with asthma[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):502.
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哮喘患儿外周血髓系抑制细胞、IL10和IL12水平检测*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年04期
页码:
502
栏目:
应用研究
出版日期:
2013-07-20

文章信息/Info

Title:
Detection of MDSCs, IL10, and IL12 levels in peripheral blood from children with asthma
作者:
张艳丽王秀芳乔俊英雷瑞瑞宋丽刘莹
郑州大学第三附属医院儿内科 郑州 450052
Author(s):
ZHANG YanliWANG XiufangQIAO JunyingLEI RuiruiSONG LiLIU Ying
Department of Pediatrics, the Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
哮喘 髓系抑制细胞白细胞介素10白细胞介素12儿童
Keywords:
asthma myeloid derived suppressor cell interleukin10 interleukin12 children
分类号:
R725.6
摘要:
目的:检测哮喘患儿外周血髓系抑制细胞(MDSCs)、白细胞介素10(IL10)和白细胞介素12(IL12)的水平。方法:应用两色荧光标记技术流式细胞仪检测哮喘发作组(n=52)、哮喘缓解组(n=50)、肺炎对照组(n=45)及正常对照组(n=48)儿童外周血MDSCs,用双抗体夹心ELISA法检测血清IL10、IL12水平。结果:哮喘发作组较其他3组外周血MDSCs、血清IL10水平升高,血清IL12水平降低,差异有统计学意义(F=1 043.820、1 284.470和1 139.380,P均<0.05);肺炎对照组与正常对照组各指标差异无统计学意义(P>0.05)。哮喘发作组患儿外周血MDSCs水平与IL10呈正相关(r=0.741,P=0.007),与IL12呈负相关(r=-0.879,P=0.014)。结论:MDSCs可能通过上调IL10、下调IL12在儿童哮喘发病中起作用。
Abstract:
Aim: To investigate the percentage of myeloid derived suppressor cells(MDSCs) and interleukin10(IL10), interleukin12(IL12) levels in peripheral blood from children with asthma. Methods: Taking 48 cases of healthy children and 45 cases of pneumonia children as control, the percentage of MDSCs in peripheral blood of 52 cases of atopic asthma and 50 cases of alleviated asthma were measured by doublecolor fluorescence labeling technique flow cytometry,and the IL10 and IL12 levels in serum were measured by double antibody sandwich ELISA. Results: The percentage of MDSCs and the serum from children with atopic asthma were significantly higher than those in other 3 groups, while the IL12 levels in serum from children with atopic asthma was significantly lower(F=1 043.820,1 284.470,1 139.380,P<0.05).The difference between pneumonia and healthy control group was not obvious(P>0.05). In children with atopic asthma, the percentage of MDSCs had positive correlation with IL10 level(r=0.741,P=0.007), while had negative correlation with IL12(r=-0.879,P=0.014). Conclusion: MDSCs may play an important role in asthma through upregulating IL10 level, and downregulating IL12 level.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省卫生厅科技攻关基金资助项目201003063 女,1976年11月生,硕士,主治医生,研究方向:小儿呼吸系统疾病的基础与临床,Email:30562393@qq.com
更新日期/Last Update: 2013-07-25