[1]侯桂琴),贝维娟),杨帅),等.食管鳞癌细胞中PTEN基因表达水平及突变位点分析*[J].郑州大学学报(医学版),2013,(05):577.
 HOU Guiqin),BEI Weijuan),YANG Shuai),et al.Expression and mutation sites analysis of PTEN gene in esophageal squamous cell carcinoma cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(05):577.
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食管鳞癌细胞中PTEN基因表达水平及突变位点分析*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年05期
页码:
577
栏目:
食管癌研究
出版日期:
2013-09-02

文章信息/Info

Title:
Expression and mutation sites analysis of PTEN gene in esophageal squamous cell carcinoma cells
作者:
侯桂琴1)贝维娟1)杨帅1)王琼叶2)鲁照明1)#
1)郑州大学药学院临床药学系 郑州 450001 2)郑州大学第一附属医院消化科 郑州 450052
Author(s):
HOU Guiqin1) BEI Weijuan1) YANG Shuai1) WANG Qiongye2) LU Zhaoming1)
1)Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 4500012)Department of Gastroenterology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
食管鳞癌PTEN基因突变
Keywords:
esophageal squamous cell carcinoma PTEN gene mutation
分类号:
R735.1
摘要:
目的:探讨PTEN基因在食管鳞癌细胞中的表达水平及突变情况。方法:采用RTPCR技术检测高分化的Eca109、低分化的EC9706和EC1食管鳞癌细胞株细胞中PTEN mRNA的表达水平,并克隆PTEN基因全长,与野生型PTEN基因序列比对,分析突变情况。结果:EC1、EC9706、Eca109细胞株中PTEN mRNA的表达水平分别为(0.06±0.02)、(0.24±0.02)、(0.41±0.01),3株细胞中PTEN mRNA的表达水平差异有统计学意义(F=306.330,P<0.001),Eca109细胞中PTEN mRNA的表达水平高于EC9706和EC1细胞(P<0.05)。3株细胞中PTEN基因均存在不同程度突变,突变主要集中在外显子5和8。结论:食管鳞癌细胞中PTEN表达水平与细胞的分化程度有关,PTEN基因突变与食管鳞癌的发生发展有关。
Abstract:
Aim: To study the expression level and mutation of PTEN gene in esophageal squamous cell carcinoma (ESCC) cells. Methods:The expression of PTEN mRNA in three ESCC cell lines (EC9706,EC1 of low differentiation and Eca109 of high differentiation) was detected by RTPCR. The full length of PTEN gene in the cell lines mentioned above was cloned, and then the sequences of PTEN gene were blasted with the sequence of wide type PTEN gene on GenBank to analyze the mutation sites. Results: The expression levels of PTEN in EC1, EC9706 and Eca109 cells were(0.06±0.02),(0.24±0.02) and (0.41±0.01), and there were significant difference among the three cell lines(F=306.330,P<0.001).That was higher in Eca109 cells with well differentiation degree than those in EC9706 and EC1 cells with low differentiation degree(P<0.05). The mutations of PTEN gene in the three ESCC cell lines were found and the mutation sites mainly focused on exon 5 and 8. Conclusion: The expression level of PTEN has correlation with the differentiation degree and there are mutations of PTEN gene in ESCC.

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更新日期/Last Update: 2013-09-01