[1]潘帧婕,曹靖,李鸣,等.谷氨酸钠对PC12细胞钠离子通道蛋白1.7表达的影响*[J].郑州大学学报(医学版),2013,(05):591.
 PAN Zhenjie,CAO Jing,LI Ming,et al.Effects of sodium glutamate on sodium channel protein 1.7 subunit expression in PC12 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(05):591.
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谷氨酸钠对PC12细胞钠离子通道蛋白1.7表达的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年05期
页码:
591
栏目:
论著
出版日期:
2013-09-02

文章信息/Info

Title:
Effects of sodium glutamate on sodium channel protein 1.7 subunit expression in PC12 cells
作者:
潘帧婕曹靖李鸣任秀花王剑南邵金平臧卫东#
郑州大学基础医学院人体解剖学教研室 郑州 450001
Author(s):
PAN ZhenjieCAO JingLI MingREN XiuhuaWANG JiannanSHAO JinpingZANG Weidong
Department of Human Anatomy,College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001
关键词:
PC12细胞谷氨酸钠钠离子通道蛋白1.7SCN9A基因
Keywords:
PC12 cell sodium glutamate sodium channel protein 1.7 subunit SCN9A gene
分类号:
Q789
摘要:
目的:研究不同浓度谷氨酸钠刺激对PC12细胞钠离子通道蛋白1.7(Nav1.7)表达的影响。方法:PC12细胞解冻后,胰蛋白酶消化传代培养,对照组为普通培养基,实验组分别用终浓度为5、10、20、40 nmol/L的谷氨酸钠刺激PC12细胞14 h。14 h后收集细胞,通过免疫荧光、RTPCR、Westernblot等方法检测Nav1.7和SCN9A mRNA表达量的变化。结果:各组PC12细胞RTPCR、免疫荧光、Westernblot结果比较,差异均有统计学意义(F=11.821、61.871、23.274,P均<0.001);且除RTPCR结果5 nmol/L谷氨酸钠刺激组与对照组相比外,其余各谷氨酸钠刺激组与对照组相比均升高(P均<0.05)。结论:PC12细胞Nav1.7的表达与疼痛有关。
Abstract:
Aim: To explore the effects of sodium glutamate on sodium channel protein 1.7 subunit (Nav1.7) expression in PC12 cells. Methods:After respectively treated by 5, 10, 20, 40 nmol/L sodium glutamate to stimulate PC12 cells for 14 hours, immunofluorescence, RTPCR,and Westernblot were used to assess the change of Nav1.7 and SCN9A mRNA. Results: There were significant differences in RTPCR, immunofluorescence and Westernblot results(F=11.821, 61.871, 23.274, P<0.001). Compared with control group, the results of sodium glutamate groups significantly increased except RTPCR result of 5 nmol/L sodium glutamate group(P<0.05). Conclusion: The excitatory amino acid could enhance the expression of Nav1.7 in PC12 cells.

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更新日期/Last Update: 2013-09-01