[1]王冲),王伟琼),余建),等.Plk1磷酸化修饰Tara蛋白对HeLa细胞胞质分裂及增殖的影响*[J].郑州大学学报(医学版),2013,(06):743.
 WANG Chong),WANG Weiqiong),YU Jian),et al.Phosphorylation of Tara by Plk1 regulates proliferation and cytokinesis of HeLa cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(06):743.
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Plk1磷酸化修饰Tara蛋白对HeLa细胞胞质分裂及增殖的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年06期
页码:
743
栏目:
论著
出版日期:
2013-11-20

文章信息/Info

Title:
Phosphorylation of Tara by Plk1 regulates proliferation and cytokinesis of HeLa cells
作者:
王冲1) 王伟琼1)余建2)刘延方1)孙玲1)孙慧1)黄河2)#
1)郑州大学第一附属医院血液科 郑州 450052;2)浙江大学附属第一医院骨髓移植中心 杭州 310003
Author(s):
WANG Chong1)WANG Weiqiong1)YU Jian2)LIU Yanfang1) SUN Ling1) SUN Hui1)HUANG He2)
1)Department of Hematology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
Plk1 Tara 磷酸化 胞质分裂 细胞增殖 HeLa细胞
Keywords:
Plk1 Tara phosphorylation cytokinesis proliferation HeLa cell
分类号:
R730.231
摘要:
目的:探讨有丝分裂相关激酶Plk1磷酸化修饰Tara蛋白在调控HeLa细胞胞质分裂及增殖中的作用。方法:运用酵母双杂交、免疫共沉淀、体外沉降实验确定Plk1与Tara两种蛋白是否能够相互结合;采用体内外磷酸化实验明确Plk1对Tara蛋白的磷酸化修饰;采用免疫荧光实验及流式细胞术检测Tara蛋白的磷酸化对HeLa细胞胞质分裂及细胞增殖的影响。结果:Plk1与Tara在体内外均能结合;Tara蛋白在体内外均能够被Plk1磷酸化修饰;过量表达Tara蛋白的磷酸化突变体对HeLa细胞的胞质分裂有明显的抑制作用,细胞增殖明显受阻。结论:Plk1能够与Tara结合,并通过磷酸化修饰Tara蛋白阻碍细胞胞质分裂进程及抑制细胞增殖。
Abstract:
Aim: To investigate the effects of phosphorylation of Tara by Plk1 on cytokinesis and proliferation of cervical cancer HeLa cells. Methods:Using yeast hybridization assay, co-immunoprecipitation and GST pull-down assay to determine the domain of Plk1 and Tara binding and interaction in vitro and in vivo. To check the phosphorylation of Tara by Plk1 using in vitro phosphorylation assay. Immunoflurescence assay and flow cytometry were used to analyze the effects of phosphorylation of Tara on cytokinesis and proliferation of Hela cells. Results: Plk1 interacted with and phosphorylated Tara in vivo and in vitro. Overexpression of phospho-mimicking mutant of Tara in Hela cells resulted in significant mitosis delay. Conclusion: Plk1-mediated phosphorylation of Tara is essential for cytokinesis and proliferation of HeLa cells.

参考文献/References:

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[4] 王冲, 余建, 黄河. Plk1 磷酸化修饰 PinX1 对宫颈癌 HeLa 细胞有丝分裂及凋亡的影响[J]. 郑州大学学报:医学版, 2013, 48(3):323
[5] Thompson SL, Bakhoum SF, Compton DA. Mechanisms of chromosomal instability[J]. Curr Biol,2010, 20(6):R285
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[7] Wu ZQ, Yang X, Weber G, et al. Plk1 phosphorylation of TRF1 is essential for its binding to telomeres[J]. J Biol Chem,2008, 283(37):25503
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相似文献/References:

[1]王冲),余建),黄河)#.Plk1磷酸化修饰PinX1对宫颈癌HeLa细胞有丝分裂及凋亡的影响*[J].郑州大学学报(医学版),2013,(03):323.
 WANG Chong),YU Jian),HUANG He).Effects of PinX1 phosphorylation by Plk1 on mitosis and apoptosis of cervical cancer HeLa cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(06):323.

备注/Memo

备注/Memo:
*国家自然科学基金资助项目 30900640; 中国博士后科学基金资助项目 2012M511591
#通讯作者,男,1961年6月生,教授,主任医师,研究方向:血液系统恶性肿瘤的临床及基础,E-mail:hehuang.zju@gmail.com
更新日期/Last Update: 2013-11-20