[1]韩俊),王彬),马珂),等.褪黑素对睡眠剥夺大鼠胃黏膜损伤的保护作用*[J].郑州大学学报(医学版),2013,(06):769.
 HAN Jun),WANG Bin),MA Ke),et al.Protective effects of melatonin on gastric mucosal lesions in rats induced by sleep deprivation[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(06):769.
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褪黑素对睡眠剥夺大鼠胃黏膜损伤的保护作用*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年06期
页码:
769
栏目:
应用研究
出版日期:
2013-11-20

文章信息/Info

Title:
Protective effects of melatonin on gastric mucosal lesions in rats induced by sleep deprivation
作者:
韩俊1)王彬1)马珂2)闫昱博2)董开源2)刘芳娥3)#
1)第四军医大学药学系 西安710032;2)第四军医大学航空航天医学系 西安710032;3)第四军医大学基础部教学实验中心 西安 710032
Author(s):
HAN Jun1) WANG Bin1) MA Ke2) YAN Yubo2) DONG Kaiyuan2) LIU Fange3)
1)School of Pharmacy, the Fourth Military Medical University, Xian 710032; 2)School of Aerospace Medicine, the Fourth Military Medical University, Xian 710032; 3)Center of Teaching and Experiment, Division of Basic Medical Sciences, the Fourth Military Medical University, Xian 710032
关键词:
褪黑素 睡眠剥夺 胃黏膜溃疡 还原型谷胱甘肽 丙二醛 前列腺素E2 大鼠
Keywords:
melatonin sleep deprivation gastric mucosal lesion reduced glutathione hormone malondialdehyde prostaglandin E2 rat
分类号:
R573;R459.1
摘要:
目的:研究褪黑素对睡眠剥夺大鼠胃黏膜损伤的保护作用及机制。方法:将24只SD大鼠采用随机数字表法分为3组,分别为对照组(生理盐水腹腔注射)、模型组(生理盐水腹腔注射+睡眠剥夺)和实验组(15 mg/kg褪黑素腹腔注射+睡眠剥夺)。采用小平台水环境法建立大鼠睡眠剥夺模型,睡眠剥夺前大鼠适应环境7 d,从适应环境开始至睡眠剥夺期间继续给药。睡眠剥夺72 h后用相应试剂盒检测各组大鼠血清丙二醛(MDA)、还原型谷胱甘肽(GSH)及前列腺素E2(PEG2)含量。观察胃黏膜组织病理损伤情况并进行胃黏膜损伤指数测定。结果:3组大鼠血清MDA、GSH、PEG2及胃黏膜损伤指数比较,差异均有统计学意义(F=9.441、9.667、9.747、486.804,P均<0.05)。与对照组比较,模型组胃黏膜损伤指数升高(P<0.05),血清MDA和PEG2含量升高、GSH含量降低(P均<0.05);与模型组比较,实验组胃黏膜损伤指数降低(P<0.05),血清MDA和PEG2含量降低、GSH含量升高(P均<0.05)。结论:褪黑素可通过降低血清MDA和升高GSH减轻睡眠剥夺造成的胃黏膜损伤。
Abstract:
Aim: To study the protective effects of melatonin on gastric mucosal lesions in rats induced by sleep deprivation and the mechanism. Methods:A total of 24 SD rats were randomly allocated into 3 groups: the control group(intraperitoneal injection of normal saline), the model group(sleep deprivation+intraperitoneal injection of normal saline)and the experimental group(sleep deprivation+intraperitoneal injection of 15 mg/kg melatonin). The rats in the model group and the experimental group were set up sleep deprivation model with a small platform of water situation. The rats adapted to the situation for 7 days before sleep deprivation, and injection was kept during acclimation and the sleep deprivation days. After 72 h sleep deprivation, malondialdehyde(MDA), prostaglandin E2(PGE2)and reduced glutathione hormone(GSH)contents in serum of the rats in each group were determined, the pathological gastric mucosal lesions were observed, and gastric mucosal ulcer index was determined. Results: The contents of MDA, GSH, and PEG2 in serum and gastric mucosal ulcer index among the three groups had statistical difference(F=9.441, 9.667, 9.747, 486.804, P<0.05). Compared with the control group, in the model group the gastric mucosal ulcer index increased significantly(P<0.05),the contents of MDA and PGE2 in serum increased and GSH decreased significantly(P<0.05). Compared with the model group, in the experimental group the gastric mucosal ulcer index decreased significantly(P<0.05),the contents of MDA and PGE2 in serum decreased and GSH increased significantly(P<0.05). Conclusion: Melatonin could alleviate the gastric injury caused by sleep deprivation.The mechanism maybe involve its inhibiting the increase of MDA as well as the promotion of the increase of GSH.

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备注/Memo

备注/Memo:
*陕西省科学技术研究发展计划基金资助项目 2011K15-06-10
#通讯作者,女,1964年2月生,硕士,教授,研究方向:应激干预与治疗,E-mail:liufange@fmmu.edu.cn
更新日期/Last Update: 2013-11-20