[1]徐永飞,张建文#,暴志国,等.脂肪来源干细胞复合不同生物支架在裸鼠皮下的促血管化作用[J].郑州大学学报(医学版),2013,(06):773.
 XU Yongfei,ZHANG Jianwen,BAO Zhiguo,et al.Promoting neovascularization of adipose tissue derived stem cells compounding with different cellular scaffold in nude mice subcutaneous stratum[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(06):773.
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脂肪来源干细胞复合不同生物支架在裸鼠皮下的促血管化作用
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年06期
页码:
773
栏目:
应用研究
出版日期:
2013-11-20

文章信息/Info

Title:
Promoting neovascularization of adipose tissue derived stem cells compounding with different cellular scaffold in nude mice subcutaneous stratum
作者:
徐永飞张建文#暴志国李静怡王耀聪许颖溦 
郑州大学第一附属医院整形外科 郑州 450052
Author(s):
XU Yongfei ZHANG Jianwen BAO Zhiguo LI Jingyi WANG Yaocong XU Yingwei
Department of Plastic Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
脂肪来源干细胞 生物支架 血管化 裸鼠
Keywords:
adipose tissue derived stem cell cellular scaffold neovascularization nude mouse
分类号:
R318
摘要:
目的:探讨脂肪来源干细胞复合不同生物支架在裸鼠皮下的促血管化作用。方法:根据所选支架的不同,将实验分为4组,A组为脂肪颗粒,B组为透明质酸钠,C组为Ⅰ型胶原支架,分别按体积比1GA6FA1与25×106 mL-1的脂肪来源干细胞混合,D组为单纯脂肪来源干细胞,将4组试剂注射到12只裸鼠皮下构建软组织,每只裸鼠各注射4个点,3个月后获取相应标本,测定湿质量、存活率、微血管密度。结果:4组间湿质量、存活率、微血管密度比较,差异均有统计学意义(F=47.523、48.425和47.619,P均<0.05),且A、B、C组湿质量、存活率、微血管密度均高于D组(P均<0.05)。结论:脂肪来源干细胞复合生物支架可加速软组织血管化,且效果优于单纯脂肪来源干细胞。
Abstract:
Aim: To discuss the affection of promoting neovascularization of adipose tissue derived stem cells(ADSCs)compounding with different cellular scaffold in nude mice subcutaneous stratum.Methods:The experiment was divided into four groups depending on the selected scaffold. Group A,B and C were given fat particles, sodium hyaluronate, type Ⅰcollagen,respectively, mixed with ADSCs at 25×106 mL-1. Group D was given pure ADSCs.Four reagents were injected randomly into subcutaneous stratum of 12 nude mice to build soft tissue. Each nude muse was injected 4 points. The appropriate specimens were obtained three months later and wet weight,survival rate and microvessel density were detected.Results: The differences were statistically significant regarding the wet weight, survival rate, microvessel density among the four groups(F=47.523,48.425,47.619,P<0.05). The wet weight, survival rate, and microvessel density of group A,B and C were higher than those of group D(P<0.05).Conclusion: ADSCs compounding with cellular scaffold could promote neovascularization in subcutaneous stratum,and is better than pure ADSCs.

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备注/Memo

备注/Memo:
#通讯作者,男,1964年11月生,硕士,主任医师,研究方向:面部年轻化,E-mail:zhangjw18@126.com
更新日期/Last Update: 2013-11-20