[1]赵志鸿)△,侯迎迎),郑立运),等.艾叶乙酸乙酯提取物对HBV的抑制作用*[J].郑州大学学报(医学版),2013,(06):783.
 ZHAO Zhihong),HOU Yingying),ZHENG Liyun),et al.Anti-hepatitis B virus activities of ethyl acetate extracts from folium artemisiae argyi in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(06):783.
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艾叶乙酸乙酯提取物对HBV的抑制作用*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年06期
页码:
783
栏目:
应用研究
出版日期:
2013-11-20

文章信息/Info

Title:
Anti-hepatitis B virus activities of ethyl acetate extracts from folium artemisiae argyi in vitro
作者:
赵志鸿1)△侯迎迎2)郑立运1)王丽阳2)王桂芳1)张小俊1)张壮丽1)
1)郑州大学医药科学研究院 郑州450052;2)郑州大学药学院 郑州 450001
Author(s):
ZHAO Zhihong1) HOU Yingying2) ZHENG Liyun1) WANG Liyang2) WANG Guifang1)ZHANG Xiaojun1)ZHANG Zhuangli1)
1)Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450052
关键词:
艾叶 乙酸乙酯提取物 乙型肝炎病毒 HepG2.2.15细胞
Keywords:
folium artemisiae argyi ethyl acetate extract hepatitis B virus HepG2.2.15 cell
分类号:
R965.1
摘要:
目的:通过体外实验观察艾叶乙酸乙酯提取物对乙型肝炎病毒(简称HBV)的抑制作用。方法:取HepG2.2.15细胞,以1.6、8.0、40.0、200.0和500.0 mg/L的艾叶乙酸乙酯提取物处理72 h后,采用MTT法测定细胞生长抑制率,计算半数毒性浓度。另取HepG2.2.15细胞,分别以0、10、20和40 mg/L艾叶乙酸乙酯提取物及100 mg/L拉米夫定(阳性对照)处理3、6、9 d,采用酶联免疫法检测细胞上清液中乙肝表面抗原、e抗原,用荧光定量PCR测定HBV DNA拷贝数,分别计算抑制率和半数抑制浓度,最后计算治疗指数。结果:艾叶乙酸乙酯提取物对HepG2.2.15细胞的半数毒性浓度为104.80 mg/L,对乙肝表面抗原、e抗原及HBV DNA的半数抑制浓度分别是1.26、8.06和38.97 mg/L,并呈剂量依赖性。治疗指数分别为HBsAg 83.2、e抗原13.0和 HBV DNA 2.7。结论:艾叶乙酸乙酯提取物在体外对HBV有明显抑制作用。
Abstract:
Aim: To observe the anti-hepatitis B virus activities of ethyl acetate extracts of folium artemisiae argyi.Methods:HepG2.2.15 cells were allocated into 5 groups and given ethyl acetate extracts at 1.6,8.0,40.0,200.0 and 500.0 mg/L for 72 h; the inhibition rate of cell growth was determined by MTT and TC50 was calculated. Another HepG2.2.15 cells were allocated into 5 groups and given ethyl acetate extracts at 0,10,20 and 40 mg/L and lamivudine(positive control)at 100 mg/L for 3,6,and 9 days; ELISA was used to determine the contents of HBsAg and HBeAg in cell supernatant, and FQ-PCR was used to determine the HBV DNA copies and the inhibition rate and IC50 were calculated, and then the therapeutic index was calculated accordingly. Results: The TC50 of ethyl acetate extracts was 104.80 mg/L, and the IC50 for HBsAg, HBeAg and HBV DNA was 1.26,8.06,and 38.97 mg/L,and the therapeutic index for the above 3 indexes was 83.2, 13.0, and 2.7.Conclusion: The extracts from folium artemisiae argyi have obvious inhibitory effects on hepatitis B virus in vitro.

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备注/Memo

备注/Memo:
*河南省省属科研单位社会公益项目预研专项资金 09yy0030
△女,1965年1月生,副研究员,研究方向:新药研发,E-mail:zzh6598@163.com
更新日期/Last Update: 2013-11-20