[1]姬旭慧),张玲),张江波),等.原发性肝细胞癌细胞系和组织中ARID2的表达*[J].郑州大学学报(医学版),2013,(06):786.
 JI Xuhui),ZHANG Ling),ZHANG Jiangbo),et al.Expression of ARID2 in hepatocellular carcinoma cell lines and tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(06):786.
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原发性肝细胞癌细胞系和组织中ARID2的表达*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年06期
页码:
786
栏目:
应用研究
出版日期:
2013-11-20

文章信息/Info

Title:
Expression of ARID2 in hepatocellular carcinoma cell lines and tissue
作者:
姬旭慧1)张玲2)张江波3)陈丹丹1)刘媛1)鲁凤民3)吕全军1)#
1)郑州大学公共卫生学院营养与食品卫生学教研室 郑州 450001;2)河南省肿瘤医院肝胆胰外科 郑州 450008;3)北京大学医学部基础医学院 北京 100191
Author(s):
JI Xuhui1)ZHANG Ling2)ZHANG Jiangbo3)CHEN Dandan1)LIU Yuan1)LU Fengmin3)LV Quanjun1)
1)Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou 450001; 2)Department of Hepatopancreatobiliary Surgery, Henan Tumor Hospital, Zhengzhou 450008; 3)School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191
关键词:
人源ARID2蛋白 肝肿瘤 肝炎病毒乙型
Keywords:
ARID2 protein human liver neoplasm hepatitis B virus
分类号:
R735.7
摘要:
目的:探讨原发性肝细胞癌(HCC)细胞系和组织中ARID2 mRNA的表达与原发性HCC发生发展的关系。方法:应用实时定量荧光PCR方法检测9种HCC细胞系和组织中ARID2 mRNA的表达。结果:不同感染背景下HCC细胞中ARID2 mRNA表达水平不同。与正常肝组织相比,ARID2 mRNA在HCC癌旁组织中的表达水平下降(有HBV感染:Z=10.684,P<0.05;无HBV感染:Z=16.015,P<0.05);在无HBV感染背景的HCC组织和配对癌旁组织中ARID2 mRNA表达水平差异有统计学意义(Z=1.886,P<0.05)。不同感染背景的HCC组织中ARID2 mRNA的表达差异有统计学意义(Z=13.479,P<0.001)。结论:ARID2可能与原发性肝细胞癌的发生发展相关。
Abstract:
Aim: To investigate the alteration of ARID2 gene expression in hepatocellular carcinoma(HCC)cell lines and tissue. Methods:The mRNA level of ARID2 in HCC cell lines and primary HCC tissue was measured by quantitative real time RT-PCR. Results: ARID2 mRNA expression level altered in HCC cell lines with the different infection background. Compared with that in normal liver tissue, the expression level of ARID2 mRNA in HCC tissue was down-regulated(with HBV infection:Z=10.684,P<0.05;without HBV infection:Z=16.015,P<0.05); without the HBV infection background, the difference of expression level of ARID2 mRNA in tumor tissue and para-carcinoma tissue was statistically significant(Z=1.886,P<0.05). ARID2 mRNA expression level altered in HCC tissues with different infection background(Z=13.479,P<0.001).Conclusion: The abnormal expression in HCC suggests that ARID2 may be associated with hepatocarcinogenesis.

参考文献/References:

[1] El-Serag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis[J]. Gastroenterology, 2007, 132(7): 2557
[2] El-Serag HB. Epidemiology of viral hepatitis and hepatocellular carcinoma[J]. Gastroenterology, 2012, 142(6): 1264
[3] Iakova P, Timchenko L, Timchenko NA. Intracellular signaling and hepatocellular carcinoma[J]. Semin Cancer Biol, 2011, 21(1): 28
[4] Zhao H, Wang J, Han Y, et al. ARID2: a new tumor suppressor gene in hepatocellular carcinoma[J]. Oncotarget, 2011, 2(11): 886
[5] Manceau G, Letouze E, Guichard C, et al. Recurrent inactivating mutations of ARID2 in non-small cell lung carcinoma [J]. Int J Cancer, 2013, 132(9): 2217
[6] Huang J, Deng Q, Wang Q, et al. Exome sequencing of hepatitis B virus-associated hepatocellular carcinoma[J]. Nat Genet, 2012, 44(10): 1117
[7] Li M, Zhao H, Zhang X, et al. Inactivating mutations of the chromatin remodeling gene ARID2 in hepatocellular carcinoma[J]. Nat Genet, 2011, 43(9): 828
[8]Yan Z, Cui K, Murray DM, et al. PBAF chromatin-remodeling complex requires a novel specificity subunit, BAF200, to regulate expression of selective interferon-responsive genes [J]. Genes Dev, 2005, 19(14):1662

备注/Memo

备注/Memo:
*北京市朝阳区艾滋病和病毒性肝炎等重大传染病综合防治示范区建设研究项目 2012ZX10004-904; 首都市民健康项目培育课题 Z111107067311027
#通讯作者,男,1962年10月生,博士,教授,研究方向:营养与相关疾病,E-mail: lqjnutr@zzu.edu.cn
更新日期/Last Update: 2013-11-20