[1]王婷△,欧敏,黄友章.益肺活血复方对人肺动脉内皮细胞微结构及内分泌功能的影响*[J].郑州大学学报(医学版),2013,(06):799.
 WANG Ting,OU Min,HUANG Youzhang.Effects of Compound Yifei Huoxue on cell microstructure and secretion function of human pulmonary artery endothelial cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(06):799.
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益肺活血复方对人肺动脉内皮细胞微结构及内分泌功能的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年06期
页码:
799
栏目:
应用研究
出版日期:
2013-11-20

文章信息/Info

Title:
Effects of Compound Yifei Huoxue on cell microstructure and secretion function of human pulmonary artery endothelial cells
作者:
王婷欧敏黄友章
中国人民解放军海军总医院干部呼吸科 北京100048
Author(s):
WANG TingOU MinHUANG Youzhang
Department of Cadre Respiration Medicine, Navy General Hospital of the PLA, Beijing 100048
关键词:
益肺活血复方 低氧高二氧化碳 人肺动脉内皮细胞 细胞微结构 内皮素-1 一氧化氮 前列环素
Keywords:
Compound Yifei Huoxue hypoxia hypercapnia human pulmonary artery endothelial cell cell microstructure endothelin-1 nitric oxide prostacyclin
分类号:
R965
摘要:
目的:观察益肺活血复方对人肺动脉内皮细胞微结构及内分泌功能的影响。方法:建立人肺动脉内皮细胞低氧高二氧化碳模型;制备益肺活血复方不同给药剂量(生药10、20、40 g/kg)的含药血清;在细胞培养液中共同孵育12 h后,利用透射电镜观察细胞微结构变化,采用放射免疫法测定各组细胞培养液上清中内皮素-1(ET-1)及前列环素(PGI2)的含量,采用硝酸还原酶法测定细胞培养液中一氧化氮(NO)的含量。结果:低氧高二氧化碳条件下,人肺动脉内皮细胞微结构损伤,而中、高剂量益肺活血复方组细胞微结构损伤减轻。各组细胞培养液或培养液上清中ET-1、NO、PGI2的含量比较,差异有统计学意义(F=121.870、60.040、49.060,P均<0.001);模型组ET-1含量高于空白对照组,NO、PGI2含量低于空白对照组(P均<0.01);中、高剂量益肺活血复方组ET-1含量低于模型组,NO和PGI2含量高于模型组(P均<0.01)。结论:低氧高二氧化碳导致人肺动脉内皮细胞微结构及功能障碍,而中、高剂量益肺活血复方可减轻其结构及功能损伤的程度。
Abstract:
Aim: To investigate the effects of Compound Yifei Huoxue(CYH)on the cell microstructure and secretion function of human pulmonary artery endothelial cells. Methods:A model of hypoxia hypercapnia was established. Different doses of pharmacy(10,20,40 g/kg crude drug)were added into the culture medium to prepare serum containing CYH. After incubation for 12 h,the cell microstructure was observed by transmission electron microscopy, the levels of ET-1 and PGI2 were tested by radioimmunoassay method, and NO level was tested by nitrate reductase. Results: Hypoxic hypercapnia caused damage to the cell microstructure,while moderate and high dose CYH could alleviate the damage.There were significant differences in the levels of ET-1, NO and PGI2 among 3 CYH model group and blank control group(F=121.870, 60.040, 49.060,P<0.001). The level of ET-1 in the model group was higher than that of blank control group,but the levels of NO and PGI2 were lower(P<0.01). The levels of ET-1 in moderate and high dose CYH groups were lower than that of model group,but the levels of NO and PGI2 were higher(P<0.01). Conclusion: Hypoxia hypercapnia could cause human pulmonary artery endothelial cell microstructure damage and dysfunction.Moderate and high dose CYH could alleviate the damage.

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备注/Memo

备注/Memo:
*全军医药卫生科研基金资助项目 36040
△女, 1986年10月生,硕士,研究方向:肺动脉高压,E-mail:mary0160@163.com
更新日期/Last Update: 2013-11-20