[1]梁锐英),赵悦),徐艳丽),等.铸造钴铬合金含银抗菌涂层对L929细胞增殖及凋亡的影响*[J].郑州大学学报(医学版),2013,(06):803.
 LIANG Ruiying),ZHAO Yue),XU Yanli),et al.Influence of casting cobalt chromium alloy Ag-containing coating on L929 cell proliferation and apoptosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(06):803.
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铸造钴铬合金含银抗菌涂层对L929细胞增殖及凋亡的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2013年06期
页码:
803
栏目:
应用研究
出版日期:
2013-11-20

文章信息/Info

Title:
Influence of casting cobalt chromium alloy Ag-containing coating on L929 cell proliferation and apoptosis
作者:
梁锐英1)赵悦2)徐艳丽1)吴文慧1)﹟孟贺1)赵敏1)
1)河北联合大学口腔医学院口腔修复科 唐山 063000;2)清河县人民医院口腔科 邢台 054800
Author(s):
LIANG Ruiying1) ZHAO Yue2) XU Yanli1) WU Wenhui1) MENG He1)ZHAO Min1)
1)Department of Prosthodontics, College of Stomatology, Hebei United University, Tangshan 063000
关键词:
钴铬合金 L929细胞 含银抗菌涂层 细胞增殖 凋亡
Keywords:
cobalt chromium L929 cell Ag-containing coating cell proliferation apoptosis
分类号:
R783.1
摘要:
目的:观察铸造钴铬合金含银抗菌涂层对小鼠成纤维细胞(L929)增殖及凋亡的影响。方法:应用等离子喷涂技术制备含银抗菌涂层钴铬合金试件10个,制作不含涂层的钴铬合金试件10个作为对照,用2组试件的浸提液培养L929细胞24、48和72 h后,利用MTT法检测细胞增殖情况;通过免疫细胞化学染色观察用浸提液原液处理48 h后L929细胞中Bcl-2和Bax蛋白的表达情况。结果:经含银抗菌涂层钴铬合金试件浸提液培养后,L929细胞增殖状态与对照比较差异无统计学意义(P>0.05),浸提液对L929的细胞毒级为0~1级;细胞内Bcl-2和Bax蛋白表达水平与对照比较,差异也无统计学意义(P>0.05)。结论:钴铬合金含银抗菌涂层具有良好的细胞相容性。
Abstract:
Aim: To evaluate the influence of casting cobalt chromium alloy Ag-containing coating on L929 cell proliferation and apoptosis. Methods:Using the technique of plasma spraying, 10 pieces of casting cobalt chromium alloy Ag-containing coating specimens as experimental group and 10 pieces of cobalt chromium alloy specimens as control group were used to prepare the leaching liquor.The MTT and immunohistochemistry method was adopted to detect proliferation and the expression of Bcl-2 and Bax proteins of the L929 cells treated by the leaching liquor. Results: The cytotoxic level of the leach liquor of casting cobalt chromium alloy Ag-containing coating pieces was Level 0-1. There was no statistical differences in the proliferation of L929 cells or the expressions of Bcl-2 and Bax protein for 2 kinds of leaching liquor(P>0.05). Conclusion: The cell compatibility of Ag-containing on casting cobalt chromium alloy is well.

参考文献/References:

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备注/Memo

备注/Memo:
*唐山市科学技术研究与发展计划项目 10140201A-4
#通讯作者,女,1969年6月生,硕士,教授,主任医师,研究方向:口腔修复材料,E-mail:wuwenhui120@163.com
更新日期/Last Update: 2013-11-20