[1]刘俊保)△,岳静宇),唐引引).冬凌草甲素对EC9706细胞增殖、凋亡的影响*[J].郑州大学学报(医学版),2014,(01):8.
 LIU Junbao#,YUE Jingyu#,TANG Yinyin#.Influence of oridonin on EC9706 cell proliferation and apoptosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(01):8.
点击复制

冬凌草甲素对EC9706细胞增殖、凋亡的影响*
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年01期
页码:
8
栏目:
食管癌研究
出版日期:
2014-01-20

文章信息/Info

Title:
Influence of oridonin on EC9706 cell proliferation and apoptosis
作者:
刘俊保12)岳静宇3)唐引引3)
1)南京中医药大学第一临床医学院中西医结合科 南京 2100292)郑州大学人民医院中医科 郑州 4500033)河南中医学院第一附属医院国家重点实验室 郑州 450000
Author(s):
LIU Junbao12)#YUE Jingyu3)# TANG Yinyin3)#
1)Subject of Integrated Traditional and Western Medicine, the First Clinical College,Nanjing University of Chinese Medicine, Nanjing 2100292)Department of Traditional Chinese Medicine, the Peoples Hospital of Zhengzhou University,Zhengzhou 4500033)State Key Laboratory, the First Teaching Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000
关键词:
冬凌草甲素食管鳞状细胞癌EC9706增殖凋亡
Keywords:
oridonin esophageal squamous cell carcinoma EC9706 proliferationapoptosis
分类号:
R735.1
摘要:
目的:观察冬凌草甲素对食管鳞状细胞癌EC9706细胞增殖、凋亡的影响。方法:用MTT法检测冬凌草甲素对EC9706细胞生长的抑制作用;采用流式细胞仪技术检测冬凌草甲素对EC9706细胞周期和细胞凋亡的影响,并计算凋亡率;激光共聚焦显微镜观察不同浓度冬凌草甲素作用后EC9706细胞中游离Ca2+荧光强度的变化。结果:MTT法显示冬凌草甲素作用EC9706细胞株后,抑制率随浓度增高而增高(F=370.600,P<0.001);流式细胞仪检测结果显示,冬凌草甲素作用EC9706细胞48 h后,G1/G0期细胞均显著增多(F=24.200,P<0.001);细胞凋亡结果显示,冬凌草甲素均能明显诱导EC9706细胞凋亡(P<0.05),冬凌草甲素40 μmol/L组的作用效果最佳(F=10.214、37.820,P<0.001)。不同浓度冬凌草甲素均升高了EC9706细胞内游离Ca2+荧光强度(F=24.400,P<0.001)。结论:冬凌草甲素可抑制EC9706细胞增殖、促进细胞凋亡。
Abstract:
Aim: To study the influence of oridonin on esophageal squamous cell carcinoma EC9706 cell proliferation and apoptosis in vitro. Methods: MTT assay was used to detect the inhibition effect of oridonin on the growth of EC9706; flow cytometry was applied to detect the impact of oridonin on cell cycle and apoptosis of EC9706, and calculated the apoptosis rate of cells; change of Ca2+fluorescence intensity of EC9706 affected by different concentrations of oridonin was observed by laser scanning confocal microscope. Results: MTT assay showed that the inhibition rate in the experiment group increased with the rising of concentration(F=370.600,P<0.001) after oridonin acting on EC9706; results of cell cycle detected by flow cytometry showed that the number of cell in G0 and G1 stage increased notably after oridonin acting on EC9706 for 48 hour respectively(F=24.200,P<0.001); results of apoptosis showed that oridonin could obviously induce the apoptosis of EC9706(P<0.05), and the effect was the best when the oridonin concentration was 40 μmol/L(F=10.214,37.820,P<0.001). Different concentrations of oridonin could increase Ca2+fluorescence intensity of EC9706, and the difference was significant (F=24.400,P<0.001). Conclusion: oridonin can improve the inhibition effects on proliferation and apoptosis of EC9706 cell.

参考文献/References:

[1]李晓昀,苏敏,黄海花,等.潮汕人与广府、客家人母系遗传背景差异的分析[J].西安交通大学学报:医学版,2010,31(6):664 [2]伍玥,董金成,户彦龙,等.手术切除淋巴结数和转移淋巴结数对食管鳞状细胞癌患者生存期的影响[J].郑州大学学报:医学版,2013,48(2):152 [3]姚国栋.bcl2,bax基因与胃癌[J].中国肿瘤,2003,12(11):656 [4]Kai L,Wang ZF,Shi YL,et al.Opioid receptor antagonists increase [Ca2+]i in rat arterial smooth muscle cells in hemorrhagic shock[J].Acta Pharmacol Sin,2004,25(3):395 [5]郝亚宁,罗媛媛,张梅,等.冬凌草甲素协同马法兰对人骨髓瘤RPMI8226细胞增殖和凋亡的影响[J].吉林大学学报:医学版,2013,39(4):699 [6]谢晓原,陈俊辉,王少彬,等.冬凌草甲素诱导人食管癌SHEE细胞凋亡及其线粒体改变[J].现代肿瘤医学,2008,16(6):907 [7]杨胜利,张巧,宋爱云,等.冬凌草甲素对大鼠肺及肝原代细胞非程序DNA合成的影响[J].河南医科大学学报,2001,36(4):415 [8]崔玉,赵斌,韩培立,等.食管鳞癌中PTEN和Caspase3的表达及临床意义[J].西安交通大学学报:医学版,2006,27(4):356 [9]胡颖军,张进朝.冬凌草甲素现代药理研究进展[J].光明中医,2011,26(3):618 [10]李翔,叶利洪,李继承.冬凌草甲素抗肿瘤活性及其机制[J].细胞生物学杂志,2009,31(3):313

相似文献/References:

[1]孟青)),刘书漫),王一菱),等.食管鳞状细胞癌组织中B7H1蛋白和mRNA的表达*[J].郑州大学学报(医学版),2009,(01):48.
 MENG Qing)),LIU Shuman),WANG Yiling),et al.Expression of B7H1 mRNA and protein in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):48.
[2]吴欣爱)△,樊青霞),李鑫),等.乏氧对EC9706细胞多药耐药相关蛋白表达的影响[J].郑州大学学报(医学版),2009,(01):51.
 WU Xinai),FAN Qingxia),LI Xin),et al.Effect of hypoxia on expression of MRP1 and the mechanism in human EC9706 cell line[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):51.
[3]郑湘予,庞霞,李晟磊,等.食管鳞状细胞癌组织中CXCR4蛋白及mRNA的表达*[J].郑州大学学报(医学版),2009,(03):475.
 ZHENG Xiangyu,PANG Xia,LI Shenglei,et al.Detection of CXCR4 protein and mRNA in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):475.
[4]亓民)),李建生)#,马军),等.食管鳞状细胞癌组织中Ca125、Ca153、Ca199和Ca724的表达[J].郑州大学学报(医学版),2009,(03):478.
 QI Min)),LI Jiansheng),MA Jun),et al.Expressions of Ca125, Ca153, Ca199 and Ca724 in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):478.
[5]王琼叶),侯桂琴),王莉莉),等.雷帕霉素抑制mTOR信号通路对EC9706细胞生长及凋亡的影响*[J].郑州大学学报(医学版),2010,(03):356.
 WANG Qiongye),HOU Guiqin),WANG Lili),et al.Effects of rapamycin inhibiting mTOR signaling pathway on growth and apoptosis of esophageal squamous cell carcinoma cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):356.
[6]陆玉成,闫红霞,王莉莉,等.自分泌运动因子对EC9706、Eca109及EC1细胞丝切蛋白磷酸化的影响[J].郑州大学学报(医学版),2010,(04):537.
[7]高萍△,臧文巧,王涛.食管鳞状细胞癌组织中Livin与半胱氨酸蛋白酶3的表达[J].郑州大学学报(医学版),2010,(06):888.
[8]孙淼淼,于庆凯,马杰,等.食管鳞状细胞癌组织中Stathmin蛋白及mRNA的表达[J].郑州大学学报(医学版),2010,(06):891.
[9]周胜理),王立东)﹟,岳文彬),等.同卵双胞胎食管鳞状细胞癌患者7对临床病理特征[J].郑州大学学报(医学版),2011,(01):123.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(01):123.
[10]谷博,吉庆春#,赵富周,等.食管鳞状细胞癌组织中Pin1和mTOR蛋白的表达[J].郑州大学学报(医学版),2012,(02):150.
 GU Bo,JI Qingchun,ZHAO Fuzhou,et al.Expressions of Pin1 and mTOR in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(01):150.

备注/Memo

备注/Memo:
*河南省卫生厅5451项目2011077;△男,1966年3月生,博士研究生,主任医师,研究方向:中西医结合肿瘤学,Email:Liu371@sohu.com
更新日期/Last Update: 2014-02-20