[1]张新恒),马曜辉),单中杰)#.ADAM9特异性siRNA对肾透明细胞癌7860细胞ADAM9基因表达及体外侵袭能力的影响[J].郑州大学学报(医学版),2014,(01):59.
 ZHANG Xinheng#,MA Yaohui#,SHAN Zhongjie#.Effect of ADAM9 targeted siRNA on ADAM9 gene expression and invasion capacity of renal clear cell carcinoma 7860 cells in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(01):59.
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ADAM9特异性siRNA对肾透明细胞癌7860细胞ADAM9基因表达及体外侵袭能力的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年01期
页码:
59
栏目:
论著
出版日期:
2014-01-20

文章信息/Info

Title:
Effect of ADAM9 targeted siRNA on ADAM9 gene expression and invasion capacity of renal clear cell carcinoma 7860 cells in vitro
作者:
张新恒1)马曜辉1)单中杰2)#
1)新乡医学院研究生处 新乡 4730032)郑州人民医院泌尿外科 郑州 450003
Author(s):
ZHANG Xinheng1)#MA Yaohui1)#SHAN Zhongjie2)#
1)Office for Graduate Students, Xinxiang Medical College,Xinxiang 4730032)Department of Urology,the Peoples Hospital of Zhengzhou City, Zhengzhou 450003
关键词:
肾透明细胞癌去整合素金属蛋白酶9侵袭
Keywords:
renal clear cell carcinomaa disintegrin and metalloprotease 9invasion
分类号:
R737.11
摘要:
目的:探讨去整合素金属蛋白酶9(ADAM9)特异性siRNA对人肾透明细胞癌(RCCC)7860细胞中ADAM9基因表达及体外侵袭能力的影响。方法:设计合成ADAM9特异性siRNA。将7860细胞分4组处理,分别为正常对照组(正常培养7860细胞)、空脂质体转染组、阴性转染组(转染无义 siRNA)和ADAM9 siRNA转染组。转染24、48、72 h后,采用RTPCR法检测细胞ADAM9 mRNA的表达;转染48 h后,采用Western blot法检测细胞ADAM9蛋白的表达, 并用Transwell法检测细胞侵袭能力。结果:转染24、48、72 h后,ADAM9 siRNA转染组7860细胞ADAM9 mRNA的表达显著低于其他3组(F=47.945、180.456、60.978,P均<0.001);转染48 h后,ADAM9 siRNA转染组7860细胞ADAM9蛋白表达显著降低(F=142.816,P<0.001),穿膜细胞数显著减少(F=45.389,P<0.001)。结论:ADAM9特异性siRNA能够有效沉默7860细胞中ADAM9的表达并降低其体外侵袭能力。
Abstract:
Aim: To explore the effect of a disintegrin and metalloprotease 9(ADAM9) targeted siRNA on ADAM9 gene expression and invasion capacity of renal clear cell carcinoma 7860 cells. Methods:ADAM9 specific siRNA was designed and synthesized.7860 cells were divided into 4 group:normal cultured group, LipofectamineTM2000 transfection group, unrelated sequence siRNA (NC siRNA)transfection group and ADAM9 specific siRNA transfection group. RTPCR was applied to detect ADAM9 mRNA in 7860 cells after transfection for 24,48,and 72 h.ADAM9 protein was detected by Western blot after transfection for 48 h, and cell invasion was detected with Transwell. Results: The expression of ADAM9 mRNA in ADAM9 specific siRNA transfection group after transfection for 24,48,and 72 h were lower(F=47.945,180.456,60.978,P<0.001),and ADAM9 protein expression and invasion capacity were significantly lower after transfection for 48 h(F=142.816,45.389,P<0.001).Conclusion: ADAM9 specific siRNA can silence ADAM9 gene in 7860 cells and inhibit its invasion.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,男,1964年12月生,博士,教授,研究方向:尿路疾病,Email:shanzhongjie@vip.sina.com
更新日期/Last Update: 2014-02-20