[1]李玉山△,吉晓菲,王全先,等.特发性弱精子症患者精子中TCTE3的表达*[J].郑州大学学报(医学版),2014,(01):83.
 LI Yushan,JI Xiaofei,WANG Quanxian,et al.Expression of TCTE3 in spermatozoa of idiopathic asthenozoospermic patients[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(01):83.
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特发性弱精子症患者精子中TCTE3的表达*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年01期
页码:
83
栏目:
应用研究
出版日期:
2014-01-20

文章信息/Info

Title:
Expression of TCTE3 in spermatozoa of idiopathic asthenozoospermic patients
作者:
李玉山吉晓菲王全先杨险峰潘周辉胡耀龙
郑州大学第三附属医院人类精子库 郑州 450052
Author(s):
LI YushanJI XiaofeiWANG QuanxianYANG XianfengPAN ZhouhuiHU Yaolong
Human Sperm Bank,the Third Affiliated Hospital, Zhengzhou University,Zhengzhou 450052
关键词:
T复合体相关睾丸表达3特发性弱精子症轴丝动力蛋白
Keywords:
Tcomplex associated testis expressed 3idiopathic asthenozoospermiaaxonemal dynein
分类号:
R698.2
摘要:
目的:探讨T复合体相关睾丸表达3(TCTE3)与特发性弱精子症发生的关系。方法:采用RTPCR和Western blot方法检测正常成年男性(正常对照)和特发性弱精子症患者各30例精子标本中TCTE3 mRNA和蛋白的表达。 结果:TCTE3 mRNA和蛋白在特发性弱精子症组精子中的相对表达量均低于正常对照组(t=3.038和2.460,P=0.005和0.017)。结论:TCTE3表达的降低可能在弱精子症的发生中起着重要作用。
Abstract:
Aim:To explore the relationship of Tcomplex associated testis expressed 3(TCTE3) with idiopathic asthenozoospermia.Methods:The mRNA and protein expressions of TCTE3 in 30 cases of normal adult male and 30 cases of idiopathic asthenozoospermia semen samples were detected by using RTPCR and Western blot. Results: The relative expression levels of TCTE3 mRNA and protein in the idiopathic asthenozoospermia group were significantly lower than those in the normal control group(t=3.038 and 2.460,P=0.005 and 0.017).Conclusion: The reduced expression of TCTE3 may play an important role in the incidence of idiopathic asthenozoospermia.

参考文献/References:

[1]Nishimune Y,Tanaka H.Infertility caused by polymorphisms or mutations in spermatogenesisspecific genes[J]. J Androl,2006, 27(3): 326 [2]De Kretser DM, Baker HW. Infertility in men: recent advances and continuing controversies [J]. J Clin Endocrinol Metab,1999, 84(10): 3443 [3]Huynh T, Mollard R, Trounson A.Selected genetic factors associated with male infertility[J].Hum Reprod Update,2002,8(2):183 [4]武文斌,李玉山,冯晓霞,等.CATSPER1蛋白与特发性弱精子症关系的研究[J].中华男科学杂志,2011,17(2):110 [5]李玉山,冯晓霞,吉晓菲,等.SEPT4蛋白在特发性弱精子症患者精子中的表达[J].中华男科学杂志,2011,17(8): 699 [6]武文斌,李玉山,吉晓菲,等.TEKT4蛋白在特发性弱精子症患者精子中的表达[J].中华男科学杂志,2012,18(6):514 [7]Rashid S, Grzmil P, Drenckhahn JD, et al.Disruption of the murine dynein light chain gene Tcte33 results in asthenozoospermia[J]. Reproduction,2010,139(1):99 [8]DiBella LM, Benashki SE, Tedford HW,et al.The Tctex1/Tctex2 class of dynein light chains[J].J Biol Chem,2001,276(17):14366 [9]Neesen J,Drenckhahn JD,Tiede S,et al.Identification of the human ortholog of the tcomplexencoded protein TCTE3 and evaluation as a candidate gene for primary ciliary dyskinesia[J].Cytogenet Genome Res,2002,98(1):38 [10]Rappold GA, Trowsdale J, Lichter P. Assignment of the human homologue of the mouse tcomplex gene TCTE3 to human chromosome 6q27[J].Genomics,1992,13(4):1337 [11]Huw LY, Goldsborough AS, Willison K, et al.Tctex2: a sperm tail surface protein mapping to the tcomplex[J]. Dev Biol,1995,170(1):183

相似文献/References:

[1]李玉山,陈河涛,杨夕阳,等.TEKT3在特发性弱精子症患者精子中的表达[J].郑州大学学报(医学版),2015,(06):843.
 LI Yushan,CHEN Hetao,YANG Xiyang,et al.Expression of TEKT3 in spermatozoa of idiopathic asthenozoospermia patients[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(01):843.

备注/Memo

备注/Memo:
*郑州市科技攻关计划项目2010S0828;△男,1966年1月生,硕士,教授,研究方向:生殖免疫学,Email:liyushanrun@126.com
更新日期/Last Update: 2014-02-20