[1]张雁儒),张辉),马辉),等.BMP2基因转染对兔骨髓间充质干细胞碱性磷酸酶活性和钙含量的影响[J].郑州大学学报(医学版),2014,(01):102.
 ZHANG Yanru#,ZHANG Hui#,MA Hui#,et al.Effect of BMP2 gene transfection on alkaline phosphalase activity and calcium deposition in bone marrow mesenchymal stem cell of rabbits[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(01):102.
点击复制

BMP2基因转染对兔骨髓间充质干细胞碱性磷酸酶活性和钙含量的影响
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年01期
页码:
102
栏目:
应用研究
出版日期:
2014-01-20

文章信息/Info

Title:
Effect of BMP2 gene transfection on alkaline phosphalase activity and calcium deposition in bone marrow mesenchymal stem cell of rabbits
作者:
张雁儒12)张辉3)马辉4)王义生2)#
1)郑州大学国际教育学院医学实验中心 郑州 4500012)郑州大学第一附属医院骨科 郑州 4500523)郑州大学临床医学系 郑州 4500524)洛阳市第三人民医院骨科 471002
Author(s):
ZHANG Yanru12)# ZHANG Hui3)# MA Hui4)# WANG Yisheng2)#
1)Center of Medical Experiment, International Education School,Zhengzhou University,Zhengzhou 450001 2)Department of Orthopaedics,the First Affiliated Hospital, Zhengzhou University,Zhengzhou 450052 3)Department of Clinical Medicine,Zhengzhou University,Zhengzhou 4500524)Department of Orthopaedics,the Third Peoples Hospital of Luoyang City,Luoyang 471002
关键词:
骨形成蛋白2骨髓间充质干细胞碱性磷酸酶
Keywords:
bone morphogenetic protein 2 bone marrow mesenchymal stem cell alkaline phosphalase calcium rabbit
分类号:
R681
摘要:
目的:观察骨形成蛋白2(BMP2)基因转染对骨髓间充质干细胞(BMSCs)碱性磷酸酶(ALP)活性和钙含量的影响。方法:成年大耳白兔16只,经髂后上嵴抽取骨髓组织混合后培养、分离BMSCs。取第3代细胞,采用电转法转染pcDNA3.1BMP2质粒。在扩大培养的第7、14、21、28和35天时收集细胞,用相应试剂盒对细胞ALP活性和钙含量进行测定,在扩大培养的第21天采用RTPCR检测细胞BMP2 mRNA的表达水平。以未转染细胞作对照。结果:转染组细胞BMP2 mRNA相对表达水平为(1.652±0.082),对照组为(0.473±0.021),转染组BMSCs中BMP2 mRNA的表达明显提高(t=2.192,P=0.005)。转染组细胞内ALP活性和钙含量较对照明显提高(P<0.01)。结论:转染BMP2基因的BMSCs表现出明显的成骨活性,可以应用为骨组织工程研究的种子细胞。
Abstract:
Aim: To investigate the changes of alkaline phosphalase(ALP) activity and calcium deposition of rabbit bone marrow mesenchymal stem cells(BMSCs) transfected with BMP2 gene. Methods: The bone marrow were extracted from posterior superior iliac spine of 16 adult rabbits,and mixed to be cultured.BMSCs were obtained and transfected with pcDNA3.1BMP2.At the 7th,14th,21st,28th and 35th day during the amplification culture,ALP activity and calcium deposition were detected;at the 21st day, BMP2 mRNA was detected using RTPCR. The BMSCs without transfection were the control.Results: The expression of BMP2 mRNA was (1.652±0.082) in transfected BMSCs, and (0.473±0.021) in the control BMSCs,and the difference between them was significant(t=2.192,P=0.005). ALP activity and calcium deposition of transfected cells were higher than those of the control(P<0.01).Conclusion: BMSCs transfected with BMP2 gene show obvious osteogenic activity,and could be used as seed cells for bone engineering research.

参考文献/References:

[1]Agarwala S,Shah SB.Tenyear followup of avascular necrosis of femoral head treated with alendronate for 3 years[J].J Arthroplasty,2011,26(7):1128 [2]AbdElBarr MM,Cox JB,Antonucci MU,et al.Recombinant human bone morphogenetic protein2 as an adjunct for spine fusion in a pediatric population[J].Pediatr Neurosurg,2011,47(4):266 [3]张爱明,李青,蔡林,等.保留BMP的微脱矿骨条在脊柱融合术中的应用研究[J].生物骨科材料与临床研究,2011,8(5):23 [4]Weber D,Kotzsch A,Nickel J,et al.A silent Hbond can be mutationally activated for highaffinity interaction of BMP2 and activin type ⅡB receptor[J].BMC Struct Biol,2007,7:6 [5]Wang CK,Ho ML,Wang GJ,et al.Controlledrelease of rhBMP2 carriers in the regeneration of osteonecrotic bone[J].Biomaterials,2009,30(25):4178 [6]Mont MA,Marulanda GA,Seyler TM,et al.Core decompression and nonvascularized bone grafting for the treatment of early stage osteonecrosis of the femoral head[J].Instr Course Lect,2007,56:213 [7]Schneider W,Breitenseher M,Engel A,et al.The value of core decompression in treatment of femur head necrosis[J].Orthopade,2000,29(5):420 [8]Harris SE,Harris MA,Mahy P,et al.Expression of bone morphogenetic protein messenger RNAs by normal rat and human prostate and prostate cancer cells[J].Prostate,1994,24(4):204 [9]宫丽华,孙晓淇,刘宝岳,等.前列腺癌骨转移灶中BMP2、BMP4、BMP7的表达及临床意义[J].临床与实验病理学杂志,2012,28(8):870 [10]王维军,牛东生.外源性激素致股骨头坏死VEGF与BMP2表达的研究[J].宁夏医学杂志,2009,31(2):102 [11]Xia Y,Mei F,Duan Y,et al.Bone tissue engineering using bone marrow stromal cells and an injectable sodium alginate/gelatin scaffold[J].J Biomed Mater Res A,2012,100(4):1044 [12]Wang Y,Li Y,Mao K,et al.Alcoholinduced adipogenesis in bone and marrow: a possible mechanism for osteonecrosis[J].Clin Orthop Relat Res,2003(410):213 [13]Suh KT,Kim SW,Roh HL,et al.Decreased osteogenic differentiation of mesenchymal stem cells in alcoholinduced osteonecrosis[J].Clin Orthop Relat Res,2005(431):220 [14]Kang P,Shen B,Yang J,et al.Repairing defect and preventing collapse of canine femoral head using titanium implant enhanced by autogenous bone graft and rhBMP2[J].Connect Tissue Res,2007,48(4):171 [15]Hattori T.Experimental investigations of osteogenesis and chondrogenesis by implant of BMPfibrin glue mixture[J].Nihon Seikeigeka Gakkai zasshi,1990,64(9):824 [16]陈顺广,郑启新,郭晓东,等.TGFβ1基因转染骨髓基质干细胞促进BMP2活性多肽大鼠体内异位成骨[J].华中科技大学学报:医学版,2009,38(1):44

相似文献/References:

[1]王义生△,韩枫,曹亚伟,等.山羊骨髓间充质干细胞的体外培养与生物学特性[J].郑州大学学报(医学版),2010,(02):266.
 WANG Yisheng,HAN Feng,CAO Yawei,et al.Biologic characteristics of goat bone marrow mesenchymal stem cells cultured in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):266.
[2]张勇),程敬亮)#,李华丽),等.超顺磁性氧化铁颗粒标记对大鼠骨髓间充质干细胞活力的影响*[J].郑州大学学报(医学版),2009,(06):1176.
 ZHANG Yong),CHENG Jingliang),LI Huali),et al.Effects of super paramagnetic iron oxide labeling on biological activity of bone marrow stem cells of rat[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):1176.
[3]牛德亮),常彩红)#.人工模拟胰腺微环境诱导骨髓间充质干细胞分化为胰岛素产生细胞*[J].郑州大学学报(医学版),2009,(05):960.
 NIU Deliang),CHANG Caihong).Mesenchymal stem cells transdifferentiate to insulinproducing cells upon microenvironment manipulation in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):960.
[4]彭涛,滕军放,关文娟,等.盐酸法舒地尔体外诱导大鼠骨髓间充质干细胞向神经元样细胞分化的可行性[J].郑州大学学报(医学版),2010,(04):559.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):559.
[5]蒋伟,法宪恩,李晓召.窦房结细胞裂解液对大鼠骨髓间充质干细胞的诱导分化作用[J].郑州大学学报(医学版),2011,(01):79.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(01):79.
[6]王义生)△,张振),张鑫),等.乙醇对兔骨髓间充质干细胞6种神经递质mRNA表达的影响*[J].郑州大学学报(医学版),2011,(03):357.
 WANG Yisheng),ZHANG Zhen),ZHANG Xin),et al.Effects of alcohol on expressions of 6 kinds of neurotransmitters mRNA in bone marrow mesenchymal stem cells of rabbits[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(01):357.
[7]武俊芳),牛杰),李晓鹏),等.氧化型低密度脂蛋白对小鼠骨髓间充质干细胞迁移的影响*[J].郑州大学学报(医学版),2014,(03):304.
 WU Junfang),NIU Jie),LI Xiaopeng),et al.Effect of oxidized lowdensity lipoprotein on migration of mouse bone marrowderived stem cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(01):304.
[8]武俊芳),牛杰),李晓鹏),等.血管紧张素Ⅱ对小鼠骨髓间充质干细胞中ERK1/2和NFκB信号途径介导的炎症反应的影响*[J].郑州大学学报(医学版),2014,(04):465.
 WU Junfang,NIU Jie,LI Xiaopeng,et al.Effect of angiotensin Ⅱ on inflammatory responses of mouse bone marrow mesenchymal stem cell mediated by ERK1/2 and NFκB pathways[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(01):465.
[9]路平),赵潇丹),郝玉娟),等.小鼠骨髓间充质干细胞在子宫内膜损伤小鼠子宫内膜中的定位*[J].郑州大学学报(医学版),2015,(01):101.
 LU Ping),ZHAO Xiaodan),HAO Yujuan),et al.Location of murine bone marrow mesenchymal stem cells in uterine endometrium of murine endometrial damage model[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(01):101.
[10]徐玉生,崔浩,张松,等.miRNA let7b修饰的骨髓间充质干细胞移植对大鼠脊髓损伤后脊髓NF200、GFAP表达的影响[J].郑州大学学报(医学版),2015,(03):400.
 XU Yusheng,CUI Hao,ZHANG Song,et al.Effects of miRNA let7bmodified bone mesenchymal stem cells transplantation on expressions of NF200 and GFAP in rats with spinal cord injury[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(01):400.

备注/Memo

备注/Memo:
#通讯作者,男,1951年2月生,硕士,教授,研究方向:骨坏死的发病机制与防治、关节脊柱外科,Email:wangyisheng@zzu.edu.cn
更新日期/Last Update: 2014-02-20