[1]李玮),李坤阳),范云),等.富血小板纤维蛋白对人牙周膜成纤维细胞迁移及成骨分化的影响[J].郑州大学学报(医学版),2014,(01):108.
 LI Wei#,LI Kunyang#,FAN Yun#,et al.Effects of plateletrich fibrin on human periodontal ligament cells migration and osteogenetic differentiation[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(01):108.
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富血小板纤维蛋白对人牙周膜成纤维细胞迁移及成骨分化的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年01期
页码:
108
栏目:
应用研究
出版日期:
2014-01-20

文章信息/Info

Title:
Effects of plateletrich fibrin on human periodontal ligament cells migration and osteogenetic differentiation
作者:
李玮1)李坤阳2)范云3)余方方3)陈栋3)#
1)郑州人民医院颐和医院口腔科 郑州 4500082)郑州市口腔医院牙周科 郑州 4500003)郑州大学口腔医学院 郑州 450052
Author(s):
LI Wei1)#LI Kunyang2)#FAN Yun3)#YU Fangfang3)#CHEN Dong3)#
1)Department of Stomatology, Yihe Hospital, Zhengzhou Peoples Hospital, Zhengzhou 4500082)Department of Periodontology, Stomatological Hospital of Zhengzhou City,Zhengzhou 4500003)School of Stomatology, Zhengzhou University,Zhengzhou 450052
关键词:
牙周膜成纤维细胞富血小板纤维蛋白迁移成骨分化
Keywords:
periodontal ligament cell plateletrich fibrin migration osteogenetic differentiation
分类号:
R781.4
摘要:
目的:观察Choukrouns富血小板纤维蛋白(PRF)对自体人牙周膜成纤维细胞(hPDLCs)迁移、成骨分化的影响,探讨PRF在牙周组织再生治疗中的潜能。方法:原代培养hPDLCs;制备PRF,实验分为P1组(1片PRF浸出液)、P2组(2片PRF浸出液)和对照组,划痕实验观察记录各组第24、48、72 h细胞迁移的距离;Transwell制备迁移模型,按实验分组培养24 h后结晶紫染色观察;成骨矿化诱导液制备不同浓度的PRF浸出液(P1组、P2组),碱性磷酸酶(ALP)试剂盒检测3、5、7 d细胞破碎液中ALP活性;成骨矿化诱导液连续培养21 d,茜素红染色后测定矿化结节面积。结果:划痕实验中P1组、P2组细胞迁移距离大于对照组(F=316.248、32.846和1 169.847,P均<0.001),P1组、P2组比较差异无统计学意义(P均>0.05)。Transwell 迁移实验中P1组、P2组与对照组比较,迁移细胞数增加(F=742.729,P<0.001),P1组、P2组比较差异无统计学意义(P>0.05)。ALP活性P1组、P2组与对照组比较差异均有统计学意义(F=474.202、1 383.521、2 317.965,P均<0.001),P1组、P2组比较差异无统计学意义(P均>0.05)。P1组、P2组与对照组矿化结节面积比较差异有统计学意义(F=332.280,P<0.001),P1组与P2组比较差异无统计学意义(P>0.05)。结论:PRF对hPDLCs具有促进其迁移和成骨分化的作用,提示PRF在牙周组织再生工程中具有很大的临床应用潜能。
Abstract:
Aim: To investigate the effects of plateletrich fibrin(PRF) on the migration and osteogenetic differentiation of human periodontal ligament cells(hPDLCs), and to evaluate the potential value of PRF used in periodontal tissue regeneration. Methods: The hPDLCs were primarily cultured and the PRF was prepared. There were three groups: 1 piece of PRF group (P1 group), 2 pieces of PRF group (P2 group), and control group. The cell migration distances at the 24th, 48th, 72th h were recorded under microscope in scratches experiment. Transwell was used to prepare migration model, and the results were observed by crystal violet staining after 24 h. Alkaline phosphatase(ALP) activity was detected by the kits of ALP at three time points. hPDLCs were induced differentiation by osteogenesis mineralization fluid and to observe mineralized nodules after being cultured for 21 days. Results: Scratch experimental results showed that the cell migration distances of the experimental groups were significantly greater than that of the control group (F=316.248,32.846, and 1 169.847, P<0.001), but there was no significant difference between two experimental groups (P>0.05). Transwell migrating experiment showed that compared with control group, the cells of the experimental groups migrating to the outside of the Transwell chambers were significantly more than that of control group (F=742.729,P<0.001), but there was no significant difference between the two experimental groups (P>0.05). The ALP activity of P1 group and P2 group were significantly more than that of control group (F=474.202,1 383.521,2 317.965,P<0.001), but there was no significant difference between P1 group and P2 group (P>0.05). There were significant differences in mineralized nodule area between experimental groups and control group (F=332.280,P<0.001), but there was no significant difference between P1 group and P2 group(P>0.05). Conclusion: PRF could promote hPDLCs migration and osteogenetic differentiation, and PRF may have great potential value in clinical application of periodontal tissue engineering.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,男,1971年10月生,博士,副主任医师,研究方向:牙周病病因防治,Email:chendongfmmu@163.com
更新日期/Last Update: 2014-02-20