[1]张岚),高冬玲),温洪涛),等.BclXL反义寡核苷酸对食管癌EC9706细胞和裸鼠移植瘤组织的诱凋亡作用*[J].郑州大学学报(医学版),2014,(02):146.
 ZHANG Lan),GAO Dongling),WEN Hongtao),et al.Induced effects of apoptosis on EC9706 cells and xenograft tissue by BclXL antisense oligodeoxynucleotide[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(02):146.
点击复制

BclXL反义寡核苷酸对食管癌EC9706细胞和裸鼠移植瘤组织的诱凋亡作用*
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年02期
页码:
146
栏目:
食管癌研究
出版日期:
2014-03-20

文章信息/Info

Title:
Induced effects of apoptosis on EC9706 cells and xenograft tissue by BclXL antisense oligodeoxynucleotide
作者:
张岚12)高冬玲2)温洪涛3)张蕾4)#
1)郑州大学基础医学院病理学教研室 郑州 450001 2)郑州大学第一附属医院病理科 郑州 450052 3)郑州大学第一附属医院消化内科 郑州 450052 4)郑州大学第一附属医院肿瘤科 郑州 450052
Author(s):
ZHANG Lan12) GAO Dongling2) WEN Hongtao3) ZHANG Lei4)
1)Department of Pathology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001 2)Department of Pathology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 3)Department of Gastroenterology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 4)Department of Oncology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
食管癌凋亡BclXL反义寡核苷酸EC9706细胞裸鼠
Keywords:
esophageal cancer apoptosis BclXL antisense oligodeoxynucleotideEC9706 cellnude mouse
分类号:
A
文献标志码:
R735.1
摘要:
目的:探讨BclXL反义寡核苷酸(BclXL ASODN)对食管癌EC9706细胞和人食管癌裸鼠移植瘤组织的诱凋亡作用。方法:将合成的BclXL ASODN通过阳离子脂质体介导转染EC9706细胞后,以吖啶橙荧光染色、流式细胞术和TUNEL技术检测ASODN组、细胞对照组、空白对照组和NODN组的凋亡率;用TUNEL技术检测ASODN组、NODN组和细胞对照组裸鼠移植瘤组织的细胞凋亡率。结果:吖啶橙荧光染色、流式细胞术和TUNEL检测结果显示,ASODN组、NODN组、空白对照组和细胞对照组的凋亡率比较,差异均有统计学意义(F=26.853、38.537、28.541,P均<0.001);且ASODN组的凋亡率均高于细胞对照组、空白对照组和NODN组(P均<0.05);TUNEL法检测移植瘤组织的细胞凋亡率结果显示,细胞对照组、NODN组及ASODN组的凋亡率比较,差异有统计学意义(F=56.375,P<0.001);且ASODN组高于细胞对照组及NODN组(P均<0.05)。结论:BclXL ASODN对食管癌EC9706细胞及食管癌裸鼠移植瘤组织均有诱凋亡作用。
Abstract:
Aim: To investigate the effects of inducing apoptosis on esophageal cancer EC9706 cells and xenograft tissue of human esophageal cancer in nude mice treated by BclXL antisense oligodeoxynucleotide(ASODN). Methods: BclXL ASODN was transfected into EC9706 cells following cationic liposome(ASODN group). NODN group, the cell control group and the blank control group were the control. The apoptosis rate of each cell groups was detected by acridine orange(AO) fluorescent staining, flow cytometry and TUNEL, respectively. The apoptosis rate in xenograft tissue from nude mice in ASODN group, NODN group and the cell control group was detected by TUNEL. Results: The results of AO fluorescent staining, flow cytometry and TUNEL showed that there were significant differences in apoptosis rates among ASODN group,the cell control group,the blank control group and NODN group(F=26.853,38.537,28.541,P<0.001). The apoptosis rate was higher in ASODN group compared with the cell control,the blank control and NODN groups(P<0.05). The apoptosis rates in xenograft tissue detected by TUNEL showed that there were significant differences among ASODN group, the cell control group and NODN group(F=56.375,P<0.001).The apoptosis rate was higher in ASODN group compared with the cell control group and NODN group(P<0.05).Conclusion: BclXL ASODN could effectively induce apoptosis of EC9706 cell and xenograft tissue of human esophageal carcinoma.

参考文献/References:

[1]JinSong Y,ZhaoXia W,ChengYu L,et al.Prognostic significance of BclxL gene expression in human colorectal cancer[J].Acta Histochem,2011,113(8):810 [2]Zhou WH,Tang F,Xu J,et al.Low expression of Beclin 1, associated with high BclxL, predicts a malignant phenotype and poor prognosis of gastric cancer[J].Autophagy,2012,8(3):389 [3]Masui T,Hosotani R,Ito D,et al.BclXL antisense oligonucleotides coupled with antennapedia enhances radiationinduced apoptosis in pancreatic cancer[J].Surgery,2006,140(2):149 [4]Leech SH,Olie RA,Gautschi O,et al.Induction of apoptosis in lungcancer cells following bclxL antisense treatment[J].Int J Cancer,2000,86(4):570 [5]Yeo AT, Porco JA Jr, Gilmore TD. BclxL, not bcl2, can protect human Blymphoma cell lines from parthenolideinduced apoptosis[J].Cancer Lett, 2012,318(1):53 [6]Kang MH,Reynolds CP.Bcl2 inhibitors: targeting mitochondrial apoptotic pathways in cancer therapy[J].Clin Cancer Res,2009,15(4):1126 [7]Shimizu S,Takehara T,Hikita H,et al.The let7 family of microRNAs inhibits BclxL expression and potentiates sorafenibinduced apoptosis in human hepatocellular carcinoma[J].J Hepatol,2010,52(5):698 [8]Wiwanitkit V.Prognostic significance of BclxL gene expression in human colorectal cancer[J].Acta Histochem,2012,114(2):182 [9]Yoshimine S,Kikuchi E,Kosaka T,et al.Prognostic significance of BclxL expression and efficacy of BclxL targeting therapy in urothelial carcinoma[J].Br J Cancer,2013,108(11):2312 [10]Kim HO,Kim E,An Y,et al.A biodegradable polymersome containing BclxL siRNA and doxorubicin as a dual delivery vehicle for a synergistic anticancer effect[J].Macromol Biosci,2013,13(6):745 [11]Hikita H,Takehara T,Shimizu S,et al.The BclxL inhibitor, ABT737, efficiently induces apoptosis and suppresses growth of hepatoma cells in combination with sorafenib[J].Hepatology,2010,52(4):1310 [12]Okumura M,Kajiyama Y,Takeda K,et al.Correlation between loss of BclXL expression and improved prognosis in advanced esophageal cancer treated by preoperative chemoradiotherapy[J].Eur Surg Res,2008,41(3):260 [13]Lieber J,Kirchner B,Eicher C,et al.Inhibition of Bcl2 and BclX enhances chemotherapy sensitivity in hepatoblastoma cells[J].Pediatr Blood Cancer,2010,55(6):1089 [14]Xie YE, Tang EJ, Zhang DR,et al. Downregulation of BclXL by RNA interference suppresses cell growth and induces apoptosis in human esophageal cancer cells[J].World J Gastroenterol,2006,12(46):7472 [15]Zhu YP,Li DC,Feng HY,et al.Antitumor effect of adenovirusmediated BclXL shRNA in vitro[J].Zhonghua Wei Chang Wai Ke Za Zhi,2012,15(3):292 [16]张蕾,温洪涛,张岚,等.BclXL反义寡核苷酸转染对食管癌细胞增殖及裸鼠体内人食管癌移植瘤生长的抑制作用[J].中华病理学杂志,2005,34(7):402

相似文献/References:

[1]张威,陈玉,亚国伟,等.RNA干扰对EC9706细胞mTOR表达及细胞生长与凋亡的影响*[J].郑州大学学报(医学版),2010,(01):11.
 ZHANG Wei,CHEN Yu,YA Guowei,et al.Effect of mTOR siRNA on the expression of mTOR gene,growth and apoptosis of human esophageal carcinoma EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(02):11.
[2]亚国伟,张威,陈玉,等.PTEN反义寡核苷酸对EC9706细胞增殖、凋亡和PTEN、mTOR表达的影响*[J].郑州大学学报(医学版),2010,(01):16.
 YA Guowei,ZHANG Wei,CHEN Yu,et al.Changes of proliferation, apoptosis and expression of mTOR and PTEN in EC9706 cells after gene PTEN inhibition by antisense oligonucleotides[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(02):16.
[3]刘桂举,梅家转#,栗敏,等.细胞因子诱导的杀伤细胞免疫表型及其对EC9706细胞杀伤活性的影响*[J].郑州大学学报(医学版),2010,(01):24.
 LIU Guiju,MEI Jiazhuan,LI Min,et al.Immunophenotype of cytokineinduced killer cells affect cytotoxic activity against EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(02):24.
[4]黄作平)),何滢),周新华),等.CD99基因转染对霍奇金淋巴瘤L428细胞株表型、细胞周期与凋亡的影响*[J].郑州大学学报(医学版),2010,(01):30.
 HUANG Zuoping),HE Ying),ZHOU Xinhua),et al.Effects of CD99 gene transfection on the phenotype,cell cycle distribution and apoptosis of L428 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(02):30.
[5]马俊芬,李沛,黄幼田,等.曲古菌素A对食管癌细胞系EC1细胞凋亡的影响*[J].郑州大学学报(医学版),2010,(02):180.
 MA Junfen,LI Pei,HUANG Youtian,et al.Effects of trichostatin A on apoptosis in esophageal carcinoma cell line EC1 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(02):180.
[6]张东铭),张苏河)#,徐占稳),等.糖尿病大鼠心肌梗死后非梗死区心肌细胞凋亡和增殖检测[J].郑州大学学报(医学版),2010,(02):259.
 ZHANG Dongming),ZHANG Suhe),XU Zhanwen),et al.Study of apoptosis and proliferation of myocardium in noninfrarction area after myocardial infraction of rat model of diabetes mellitus[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(02):259.
[7]舒建昌△,朱海燕,吴海恩,等.姜黄素对人肝星状细胞形态学的影响*[J].郑州大学学报(医学版),2009,(06):1141.
 SHU Jianchang,ZHU Haiyan,WU Haien,et al.Effects of curcumin on morphology of human hepatic stellate cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(02):1141.
[8]高红伟),李印),郭长青)#,等.腺病毒介导的干扰素β基因对SMMC7721细胞凋亡的影响*[J].郑州大学学报(医学版),2009,(06):1178.
 GAO Hongwei),LI Yin),GUO Changqing),et al.Effects of adenovirusmediated interferonβ gene on apoptosis of SMMC7721 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(02):1178.
[9]罗执芬,周云#,刘明月.紫杉醇联合替尼泊苷对胃癌BGC823细胞增殖和凋亡的影响[J].郑州大学学报(医学版),2009,(05):942.
 LUO Zhifen,ZHOU Yun,LIU Mingyue.Effects of taxol and VM26 on proliferation and apoptosis of gastric cancer cell line BGC823[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(02):942.
[10]周云△,罗执芬.5氟尿嘧啶联合替尼泊苷对胃癌BGC823细胞增殖和凋亡的影响*[J].郑州大学学报(医学版),2009,(05):945.
 ZHOU Yun,LUO Zhifen.Effects of 5FU and VM26 on proliferation and apoptosis of gastric cancer cell line BGC823[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(02):945.
[11]常凯杰),田芳),闫苒),等.吡咯烷二硫代氨基甲酸对食管癌细胞系EC1增殖、凋亡及细胞周期的影响*[J].郑州大学学报(医学版),2012,(01):4.
 CHANG Kaijie,TIAN Fang,YAN Ran,et al.Effects of pyrrolidine dithiocarbamate on the proliferation, apoptosis and cell cycle in esophageal cancer EC1 cell line[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(02):4.
[12]黄丽艳),王娜),汤黎明),等.PTEN基因表达对EC9706细胞增殖、侵袭、凋亡能力的影响*[J].郑州大学学报(医学版),2013,(01):16.
 HUANG Liyan,WANG Na,TANG Liming,et al.Effects of PTEN on proliferation,invasion and apoptosis in EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(02):16.

备注/Memo

备注/Memo:
*河南省高校杰出科研人才创新工程基金资助项目2007KYCX005 #通讯作者,女,1968年6月生,博士,教授,主任医师,研究方向:肿瘤靶向治疗,Email:ZL2909@163.com
更新日期/Last Update: 2014-04-23