[1]何光耀,谢貌,唐安洲#,等.苦参碱对人鼻咽癌CNE2细胞裸鼠移植瘤生长及凋亡相关基因表达的影响*[J].郑州大学学报(医学版),2014,(02):169.
 HE Guangyao,XIE Mao,TANG Anzhou,et al.Effect of matrine on growth and apoptosisrelated genes expressions of human nasopharyngeal carcinoma CNE2 cell xenografts in nude mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(02):169.
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苦参碱对人鼻咽癌CNE2细胞裸鼠移植瘤生长及凋亡相关基因表达的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年02期
页码:
169
栏目:
论著
出版日期:
2014-03-20

文章信息/Info

Title:
Effect of matrine on growth and apoptosisrelated genes expressions of human nasopharyngeal carcinoma CNE2 cell xenografts in nude mice
作者:
何光耀谢貌唐安洲#谭颂华
广西医科大学第一附属医院耳鼻咽喉头颈外科 南宁 530021
Author(s):
HE Guangyao XIE Mao TANG Anzhou TAN Songhua
Department of OtolaryngologyHeadNeck Surgery,the First Affiliated Hospital, Guangxi Medical University,Nanning 530021
关键词:
苦参碱鼻咽癌凋亡移植瘤裸鼠
Keywords:
matrinenasopharyngeal carcinomaapoptosisxenograftnude mice
分类号:
A
文献标志码:
R730.5
摘要:
目的:观察苦参碱对人鼻咽癌CNE2细胞裸鼠移植瘤生长及凋亡相关基因表达的影响。方法:20只裸鼠皮下接种CNE2,建立移植瘤模型,成瘤后,随机分成4组,每组5只。苦参碱高、低剂量组分别腹腔注射100、50 mg/kg苦参碱,阳性对照组腹腔注射顺铂2 mg/kg,阴性对照组给予生理盐水。给药7次后,应用体内抑瘤实验测定抑瘤率,分别采用realtime PCR法和Western blot法检测移植瘤组织中bax、bcl2、caspase3 mRNA和蛋白的表达。结果:阳性对照组和苦参碱低、高剂量组抑瘤率分别为51.6%和26.4%、43.2%。4组间移植瘤组织中bax、bcl2、caspase3的mRNA和蛋白表达差异有统计学意义(F=3.751、16.113、3.918和26.500、9.771、12.255,P<0.05);与阴性对照组比较,阳性对照组和苦参碱高、低剂量组bax mRNA和蛋白表达及阳性对照组、苦参碱高剂量组caspase3 mRNA和蛋白表达上调(P<0.05),bcl2 mRNA和蛋白表达下调(P<0.05)。结论:苦参碱可通过调节CNE2移植瘤组织中凋亡相关基因的表达抑制肿瘤的生长。
Abstract:
Aim: To investigate the effect of matrine on expressions of apoptosisrelated genes and growth of human nasopharyngeal carcinoma CNE2 cell xenografts in nude mice. Methods: A total of 20 nude mice were subcutaneously inoculated with CNE2.After the successful establishment of xenograft model,the 20 nude mice were randomly divided into 4 groups, 5 rats in each group. Low and highdose matrine groups were intraperitoneally injected with matrine at dose of 50 and 100 mg/kg, positive control group was injected with cisplatin,and negative control group received normal saline. After 7 times administration, the tumor inhibition rate was detected,and the mRNA and protein expressions of bax, bcl2 and caspase3 were detected by realtime PCR and Western blot,respectively. Results: The tumor inhibition rates of positive control group, and low,highdose matrine groups were 51.6%, 26.4%, and 43.2%;the mRNA and protein expressions of bax,bcl2 and caspase3 in xenograft tissue had significant differences(F=3.751,16.113,3.918 and 26.500,9.771,12.255, P<0.05). Compared with the negative control group, the mRNA and protein expressions of bax in positive control group and low,highdose matrine groups and those of caspase3 in positive control group and highdose matrine group increased(P<0.05),but those of bcl2 decreased(P<0.05). Conclusion: Matrine could inhibit the growth of CNE2 xenograft through regulating the expressions of apoptosisrelated genes.

参考文献/References:

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目81241113;广西教育厅科研立项项目201106LX088 #通讯作者,男,1962年3月生,教授,研究方向:抗鼻咽癌中药研究,Email:anzhoutang@126.com
更新日期/Last Update: 2014-04-23