[1]王继慧),张小卿),马月丹),等.绞股蓝总皂苷对光老化人皮肤成纤维细胞凋亡的影响*[J].郑州大学学报(医学版),2014,(02):194.
 WANG Jihui),ZHANG Xiaoqing),MA Yuedan),et al.Effects of gypenosides on the apoptosis of photoaging human dermal fibroblasts[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(02):194.
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绞股蓝总皂苷对光老化人皮肤成纤维细胞凋亡的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年02期
页码:
194
栏目:
论著
出版日期:
2014-03-20

文章信息/Info

Title:
Effects of gypenosides on the apoptosis of photoaging human dermal fibroblasts
作者:
王继慧12)张小卿3)马月丹4)张燚5)马贤德6)吴景东7)#
1)辽宁中医药大学基础医学院 沈阳 110847 2)河南财政税务高等专科学校对外经济贸易系 郑州 451464 3)辽宁中医药大学针灸推拿学院 沈阳 110847 4)辽宁中医药大学经济管理学院 沈阳 110847 5)辽宁中医药大学附属第三医院辽宁省肛肠医院皮肤科 沈阳 110003 6)辽宁中医药大学教学实验中心 沈阳 110847 7)辽宁中医药大学学生工作处 沈阳 110847
Author(s):
WANG Jihui12) ZHANG Xiaoqing3) MA Yuedan4) ZHANG Yi5) MA Xiande6) WU Jingdong7)
1)Basic Medical School, Liaoning University of Traditional Chinese Medicine, Shenyang 110847 2)Department of International Trade and Economics, Henan College of Finance and Taxation, Zhengzhou 451464 3)Acupuncture and Massage College, Liaoning University of Traditional Chinese Medicine, Shenyang 110847 4)School of Economics and Management, Liaoning University of Traditional Chinese Medicine, Shenyang 110847 5)Department of Dermatology,Liaoning Provincial Coloproctological Hospital; the Third Affiliated Hospital, Liaoning University of Traditional Chinese Medicine, Shenyang 110003 6)Teaching Experiment Center, Liaoning University of Traditional Chinese Medicine, Shenyang 110847 7)Department of Student Work, Liaoning University of Traditional Chinese Medicine, Shenyang 110847
关键词:
绞股蓝光老化皮肤成纤维细胞凋亡Bcl2Bax
Keywords:
gypenosidesphotoagingdermal fibroblastapoptosisBcl2Bax
分类号:
A
文献标志码:
R229
摘要:
摘要目的:观察绞股蓝总皂苷(Gyp)对光老化人皮肤成纤维细胞株HSF凋亡的影响。方法:分别按80、160和320 mg/d的剂量灌胃大鼠Gyp生理盐水溶液,7 d后取血,分离血清备用。采用长波紫外线(UVA)照射方法建立光老化HSF细胞模型,照射剂量36 J/cm2,然后用制备的低、中、高剂量Gyp含药血清进行干预培养。12 h后,采用TUNEL法检测细胞凋亡情况,采用RTPCR和Western blot法检测细胞中Bax、Bcl2 mRNA和蛋白的表达。以未照射细胞(空白对照组)、模型细胞和正常血清干预的模型细胞(正常血清组)作对照。结果:6组凋亡细胞数,细胞中Bcl2、Bax mRNA和蛋白的表达水平差异均有统计学意义(F=474.201,351.094、334.907和403.610、162.766,P<0.001)。与空白对照组比较,UVA模型组和正常血清组细胞凋亡增强,Bcl2 mRNA和蛋白表达降低,同时Bax mRNA和蛋白表达升高(P<0.05)。与UVA模型组和正常血清组比较,低、中、高剂量Gyp组细胞凋亡减弱,细胞中Bcl2 mRNA和蛋白表达升高,Bax mRNA和蛋白表达降低(P<0.05),高剂量组效果更显著(P<0.05)。结论:Gyp可以逆转UVA诱导的人皮肤成纤维细胞的凋亡。
Abstract:
Aim: To investigate the effect of gypenosides(Gyp) on the apoptosis of photoaging human dermal fibroblasts(HSF cells) induced by ultraviolet A(UVA) radiation. Methods: Rats were intragastricly administrated Gyp saline solution at doses of 80, 160 and 320 mg/d, respectively.7 d later, the serum was isolated. HSF cells were irradiated by UVA at a dose of 35 mJ/cm2 to establish photoaging cell model,then these cells were cultured by low,median and highdose Gyp medicated serum(Gyp groups).12 h later, apoptosis was detected by TUNEL, the expressions of Bax and Bcl2 mRNA and protein in cells were detected using RTPCR and Western blot,respectively.The cells without irradiation(blank control group), model cells without intervention(model group) and with normal serum intervention (normal serum group) were the controls.Results: There were significant differences in apoptosis cell number,expressions of Bcl2 mRNA,Bax mRNA,Bcl2 and Bax protein in cells among the six groups(F=474.201,351.094,334.907 and 403.610,162.766,P<0.001).Compared with the blank control group, apoptosis of model group and normal serum group increased, and the expressions of Bcl2 mRNA and protein decreased,while those of Bax increased significantly(P<0.05). Compared with the model group and normal serum group, apoptosis of Gyp groups decreased,and the expressions of Bcl2 mRNA and protein increased,while those of Bax decreased significantly(P<0.05),especially the highdose Gyp group(P<0.05).Conclusion: Gyp can protect HSF cells from UVAinduced apoptosis.

参考文献/References:

[1]Leccia MT,Richard MJ,Favier A,et al.Zinc protects against ultraviolet A1induced DNA damage and apoptosis in cultured human fibroblasts[J].Biol Trace Elem Res,1999,69(3):177 [2]Xie H,Liu F,Liu L,et al.Protective role of AQP3 in UVAinduced NHSFs apoptosis via Bcl2 upregulation[J].Arch Dermatol Res,2013,305(5):397 [3]Lamore SD,Wondrak GT.UVA causes dual inactivation of cathepsin B and L underlying lysosomal dysfunction in human dermal fibroblasts[J].J Photochem Photobiol B,2013,123:1 [4]王大伟,吴景东.绞股蓝抗小鼠皮肤衰老的实验研究[J].四川中医,2008,26(10):17 [5]金晓哲,吴景东,闫海慧.外用中药绞股蓝对紫外线照射无毛小鼠皮肤组织影响的实验研究[J].中国生物美容,2009(4):16 [6]王彩霞,王丽新.外用绞股蓝对自然衰老小鼠皮肤组织中CAT 活性、MDA 含量影响的实验研究[J].实用中医内科杂志,2011,25(7):29 [7]李楠,谷继卜,曲桂霞.绞股蓝水煎剂对老龄小鼠SOD和LPO的抗衰老研究[J].黑龙江医药科学,2012,35 (4):41 [8]陶冶,张小卿,吴琼,等.绞股蓝总皂苷含药血清对光老化HaCaT细胞中p38MAPK蛋白和cJun mRNA表达的抑制作用[J].吉林大学学报:医学版,2012,47(10):1178 [9]李薇.长波紫外线对人角质形成细胞和成纤维细胞的形态、数目及诱导型一氧化氮合酶表达的影响[D].长沙:中南大学,2007. [10]Pimentel AA,Benaim G.Ca2+ and sphingolipids as modulators for apoptosis and cancer[J].Invest Clin,2012,53(1):84 [11]闫言,王宝玺.紫外线引起氧化应激与细胞凋亡机制研究进展[J].国外医学:皮肤性病学分册,2004,30(3):167 [12]Murphy M,Mabruk MJ,Lenane P,et al.The expression of p53,p21,Bax and induction of apoptosis in normal volunteers in response to different doses of ultraviolet radiation[J].Br J Dermatol,2002,147(1):110

备注/Memo

备注/Memo:
*辽宁省自然科学基金项目201102150 #通讯作者,男,1962年10月生,博士,教授,研究方向:中医美容、皮肤衰老与中药抗皮肤衰老研究,Email:lnzywjd0719@163.com
更新日期/Last Update: 2014-04-23