[1]赵先兰△,赵太坤,刘彩,等.子痫前期患者胎盘组织及外周血中Smac、Survivin和PTEN的表达及其相关性[J].郑州大学学报(医学版),2014,(02):198.
 ZHAO Xianlan,ZHAO Taikun,LIU Cai,et al.Expression and correlation study of Smac, Survivin and PTEN in placentae and maternal peripheral blood from patients with preeclampsia[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(02):198.
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子痫前期患者胎盘组织及外周血中Smac、Survivin和PTEN的表达及其相关性
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年02期
页码:
198
栏目:
论著
出版日期:
2014-03-20

文章信息/Info

Title:
Expression and correlation study of Smac, Survivin and PTEN in placentae and maternal peripheral blood from patients with preeclampsia
作者:
赵先兰△赵太坤刘彩刘传李贞
郑州大学第一附属医院妇产科 郑州 450052
Author(s):
ZHAO Xianlan ZHAO TaikunLIU Cai LIU Zhuan LI Zhen
Department of Obstetrics and Gynecology, the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052
关键词:
子痫前期SmacSurvivinPTEN
Keywords:
preeclampsia Smac Survivin PTEN
分类号:
A
文献标志码:
R711.7
摘要:
目的:探讨Smac、Survivin和PTEN在子痫前期患者胎盘组织和外周血中的表达及其相关性。方法:采用RTPCR、免疫组化SP法测定90例胎盘组织(其中正常孕妇组、轻度和重度子痫前期组各30例)中Smac、Survivin和PTEN mRNA和蛋白的表达,ELISA法测定外周血中Smac、Survivin和PTEN蛋白的水平。 结果:①Smac、Survivin主要定位于绒毛细胞滋养细胞胞浆中,PTEN主要定位于绒毛滋养细胞胞核中。②轻、重度子痫前期组胎盘及外周血中Smac mRNA和蛋白表达水平均高于对照组,且重度组高于轻度组,差异均有统计学意义(F=19.874和112.774,P<0.001)。③轻、重度组子痫前期组胎盘及外周血中Survivin、PTEN mRNA和蛋白表达水平均低于对照组,且重度组低于轻度组,差异均有统计学意义(F=21.306、23.485和152.751、630.848,P<0.001)。④子痫前期组胎盘和外周血中Smac与Survivin及PTEN蛋白表达呈负相关(P<0.05)。⑤子痫前期组胎盘和外周血中Smac蛋白与新生儿体重及胎盘质量呈负相关,Survivin和PTEN蛋白与新生儿体重及胎盘质量呈正相关(P<0.05)。结论:Smac高表达、Survivin和PTEN低表达在子痫前期的发生、发展中起重要作用,与新生儿预后有关。
Abstract:
Aim: To study the expressions and correlations of the mitochondrial protein Smac, antiapoptotic gene Survivin and tumor suppressor PTEN in placentae and maternal blood from patients with preeclampsia. Methods: A total of 90 placental samples (normal pregnancies=30, mild preeclampsia=30, severe preeclampsia=30) were collected. The expressions of Smac, Survivin and PTEN were measured by reverse transcriptase PCR and immunohistochemical SP method and ELISA.Results: Smac and Survivin were mainly expressed in trophoblast cytoplasm,and PTEN was mainly expressed in trophoblast nucleus. The expressions of Smac mRNA and protein in placentae and maternal peripheral blood from patients with mild and severe preeclampsia were significantly higher than those from control group,moreover,which was higher in severe preeclampsia group(F=19.874,112.774,P<0.001). The expressions of Survivin,PTEN mRNA and protein in placentae and maternal peripheral blood from patients with mild and severe preeclampsia were significantly lower than those in control group, moreover,which was lower in severe preeclampsia group(F=21.306,23.485 and 152.751,630.848,P<0.001). The expressions of Smac and Survivin,Smac and PTEN protein were negatively correlated in preeclampsia group(P<0.05). The expression of Smac protein was negatively correlated with the weight of the newborn and the placenta, while Survivin and PTEN proteins were positively correlated with the weight of the newborn and the placenta(P<0.05). Conclusion: The abnormal expressions of Smac, Survivin and PTEN may contribute to the occurrence of preeclampsia and are related to prognosis of the birth.

参考文献/References:

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备注/Memo

备注/Memo:
△女,1965年10月生,博士,主任医师,研究方向:围产医学,Email:zxl12129
更新日期/Last Update: 2014-04-23