[1]陈翠翠),叶娟),赵俊伟),等.复合抗原ESAT6/CFP10/Rv0315在活动性肺结核辅助诊断中的价值*[J].郑州大学学报(医学版),2014,(02):211.
 CHEN Cuicui,YE Juan,ZHAO Junwei,et al.Diagnostic value of multiple antigens ESAT6/CFP10/Rv0315 in detection of active pulmonary tuberculosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(02):211.
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复合抗原ESAT6/CFP10/Rv0315在活动性肺结核辅助诊断中的价值*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年02期
页码:
211
栏目:
论著
出版日期:
2014-03-20

文章信息/Info

Title:
Diagnostic value of multiple antigens ESAT6/CFP10/Rv0315 in detection of active pulmonary tuberculosis
作者:
陈翠翠1)叶娟2)赵俊伟3)张舒林3)孙战强4)#汤兵祥5)#
1)郑州大学第一附属医院呼吸内科 郑州 450052 2)河南中医学院医学免疫学重点实验室 郑州 450008 3)上海交通大学基础医学院病原生物学教研室 上海 200025 4)复旦大学生命科学学院 上海 200433 5)河南省胸科医院呼吸内科 郑州 450008
Author(s):
CHEN Cuicui1) YE Juan2) ZHAO Junwei3) ZHANG Shulin3) SUN Zhanqiang4) TANG Bingxiang5)
1)Department of Respiratory Medicine, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Key Laboratory of Medical Immunology, Henan University of Traditional Chinese Medicine, Zhengzhou 450008 3)Department of Medical Microbiology and Parasitology, College of Basic Medical Sciences,Shanghai Jiaotong University, Shanghai 200025 4)College of Life Sciences, Fudan University, Shanghai 200433 5)Department of Respiratory Medicine, Henan Chest Hospital, Zhengzhou 450008
关键词:
肺结核结核分枝杆菌ESAT6CFP10Rv0315
Keywords:
pulmonary tuberculosisMycobacterium tuberculosis ESAT6 CFP10 Rv0315
分类号:
A
文献标志码:
R521
摘要:
目的:评价结核分枝杆菌复合抗原ESAT6/CFP10/Rv0315在活动性肺结核诊断中的应用价值。方法:选取初治肺结核病患者70例和健康体检者34例作为结核组和健康对照组,分离PBMCs,分别用复合抗原ESAT6/CFP10/Rv0315、ESAT6肽段库和CFP10肽段库作为抗原进行ELISPOT实验,检测斑点形成细胞数。结果:复合抗原ESAT6/CFP10/Rv0315、ESAT6肽段库和CFP10肽段库检测结核分枝杆菌感染的阳性率依次为85.7%(60/70)、77.1%(54/70)、72.9%(51/70),特异性依次为82.4%(28/34)、85.3%(29/34)、88.2%(30/34)。结论:复合抗原ESAT6/CFP10/Rv0315作为抗原建立的ELISPOT检测方法在活动性肺结核的辅助诊断中有较高的应用价值。
Abstract:
Aim: To evaluate the application value of multiple antigens ESAT6/CFP10/Rv0315 in the detection of active pulmonary tuberculosis.Methods: A total of 70 patients with initial pulmonary tuberculosis and 34 healthy persons were enrolled as tuberculosis group and control group. PBMCs isolated from two groups were cocultured with ESAT6/CFP10/Rv0315, ESAT6 and CFP10 antigens. SFCs were enumerated by ELISPOT. Results: The sensitivity of ESAT6/CFP10/Rv0315, ESAT6 and CFP10 antigens by ELISPOT was 85.7%(60/70), 77.1%(54/70), and 72.9%(51/70), respectively. Specificity was 82.4%(28/34), 85.3%(29/34), and 88.2%(30/34), respectively. Conclusion: Multiple antigens ESAT6/CFP10/Rv0315 showed certain value for auxiliary diagnosis of active pulmonary tuberculosis.

参考文献/References:

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备注/Memo

备注/Memo:
*“十二·五”国家科技重大专项2013ZX10003002005;国家自然科学基金81271794;上海市科委科技支撑项目12441903300;河南省卫 生厅科技攻关项目201203163 #通讯作者,汤兵祥,男,1956年4月生,硕士,主任医师,研究方向:呼吸系统疾病基础及临床,Email: tangbingxiang01@sina.com;孙战强,男,1971年12月生,硕士,高级工程师,研究方向:微生物免疫学诊断,Email:sunzhanqiang@126.com
更新日期/Last Update: 2014-04-23