[1]郭燕,张东铭,刘爱萍,等.饮食干预对高脂高糖妊娠大鼠肝组织中TRB3和Akt mRNA的表达及胰岛素抵抗的影响*[J].郑州大学学报(医学版),2014,(02):230.
 GUO Yan,ZHANG Dongming,LIU Aiping,et al.Effects of dietary intervention on TRB3 and Akt mRNA expressions in hepatic tissue and insulin resistance of pregnant rats with high sucrose and fat diet[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(02):230.
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饮食干预对高脂高糖妊娠大鼠肝组织中TRB3和Akt mRNA的表达及胰岛素抵抗的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年02期
页码:
230
栏目:
应用研究
出版日期:
2014-03-20

文章信息/Info

Title:
Effects of dietary intervention on TRB3 and Akt mRNA expressions in hepatic tissue and insulin resistance of pregnant rats with high sucrose and fat diet
作者:
郭燕张东铭刘爱萍付艳芹张苏河#
郑州大学第二附属医院内分泌科 郑州 450014
Author(s):
GUO YanZHANG DongmingLIU AipingFU YanqinZHANG Suhe
Department of Endocrinology,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014
关键词:
妊娠高脂高糖饮食胰岛素抵抗Tribbles同源蛋白3Akt饮食干预大鼠
Keywords:
gestationhigh sucrose and fat dietinsulin resistencetribbles homologous protein 3Aktdietary interventionrat
文献标志码:
R587.1
摘要:
目的:观察饮食干预对高脂高糖妊娠大鼠肝组织中Tribbles同源蛋白3(TRB3)和Akt mRNA的表达及胰岛素抵抗的影响。方法:75只健康雌性SD大鼠随机分为高脂高糖妊娠组、高脂高糖非妊娠组、普通饮食妊娠组、普通饮食非妊娠组及高脂高糖饮食妊娠后普通饮食干预组(干预组)5组,每组15只。妊娠第20天,用全自动生化检测仪检测FBG,ELISA试剂盒测定FINS,由此计算HOMAIR。应用RTPCR测定大鼠肝组织中TRB3和Akt mRNA的表达水平。结果:妊娠、高脂高糖饮食均可致大鼠HOMAIR升高(F妊娠=2 318.491,F饮食=2 888.237,F交互=993.094,P均<0.001),同时大鼠肝组织中TRB3 mRNA的表达水平升高(F妊娠=256.887,F饮食=1 749.406,F交互=2.579,P均<0.001),Akt mRNA的表达水平降低(F妊娠=221.091,F饮食=1 416.984,F交互=28.918,P均<0.001)。高脂高糖妊娠组、干预组和普通饮食妊娠组HOMAIR、TRB3和Akt mRNA的表达水平差异均有统计学意义(F=2 772.745、445.896和390.334,P均<0.001);干预组HOMAIR、TRB3 mRNA的表达水平较高脂高糖妊娠组降低,但仍高于普通饮食妊娠组,Akt mRNA的表达水平较高脂高糖妊娠组升高,但仍低于普通饮食妊娠组(P<0.05)。结论:TRB3和Akt可能参与了妊娠期胰岛素抵抗的发生发展,饮食干预可改善TRB3/Akt通路,减轻妊娠期胰岛素抵抗。
Abstract:
Aim: To study the effects of dietary intervention on the expressions of tribbles homologous protein 3(TRB3) and Akt mRNA in hepatic tissue and insulin resistance(IR) of pregnant rats with high sucrose and fat diet.Methods: Seventyfive healthy female SD rats were divided into five groups randomly: high sucrose and fat diet pregnant rat(HP) group,high sucrose and fat diet virgin rat(HV) group,normal diet pregnant rat(NP) group, normal diet virgin rat(NV) group and dietary intervention pregnant rat(GHP) group.Twenty days after gestation,fasting plasma glucose and fasting insulin were measured,and IR index(HOMAIR) was calculated accordingly. RTPCR was performed to detect the expressions of TRB3 and Akt mRNA in hepatic tissue.Results: Gestation and high sucrose and fat diet could cause the increase of HOMAIR and TRB3 mRNA(HOMAIR:Fgestation=2 318.491,Fdiet=2 888.237, Finteraction=993.094,all P<0.001;TRB3 mRNA: Fgestation=256.887,Fdiet=1 749.406, Finteraction=2.579,all P<0.001),and the decrease of Akt mRNA(Fgestation=221.091,Fdiet=1 416.984, Finteraction=28.918,all P<0.001). HOMAIR and the levels of TRB3 mRNA and Akt mRNA among HP group, NP group and GHP group had significant differences. HOMAIR and the level of TRB3 mRNA in GHP group were lower than those of HP group, but still higher than those of NP group; the level of Akt mRNA was higher than that of HP group, but still lower than that of NP group(all P<0.05). Conclusion: TRB3 and Akt might be involved in the IR in gestation. Dietary intervention could decrease IR by improving TRB3/Akt signal path.

参考文献/References:

[1]Stuebe AM,Mantzoros C,Kleinman K,et al.Gestational glucose tolerance and maternal metabolic profile at 3 years postpartum[J].Obstet Gynecol,2011,118(5):1065 [2]Kato S,Du K.TRB3 modulates C2C12 differentiation by interfering with Akt activation[J].Biochem Biophys Res Commun,2007,353(4):933 [3]李变锋,付艳芹,张东铭,等.饮食干预对高脂高糖诱导的妊娠期大鼠胰岛素抵抗状态的影响[J].郑州大学学报:医学版,2013,48(4):499 [4]Holemans K,Caluwaerts S,Poston L,et al.Dietinduced obesity in the rat: a model for gestational diabetes mellitus[J].Am J Obstet Gynecol,2004,190(3):858 [5]李变锋,辛亚萍,王崇贤,等.高脂高糖喂养诱导妊娠期胰岛素抵抗大鼠模型的建立与评价[J].广东医学,2013,34(11):1667 [6]侯香华,关广聚.TRB3与胰岛素抵抗[J].中华医学杂志,2007,87(38):2730 [7]Bowers AJ,Scully S,Boylan JF.SKIP3, a novel Drosophila tribbles ortholog, is overexpressed in human tumors and is regulated by hypoxia[J].Oncogene,2003,22(18):2823 [8]Li X,Monks B,Ge Q,et al.Akt/PKB regulates hepatic metabolism by directly inhibiting PGC1alpha transcription coactivator[J].Nature,2007,447(7147):1012 [9]Du K,Herzig S,Kulkarni RN,et al.TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver[J].Science,2003,300(5625):1574 [10]He L,Simmen FA,Mehendale HM,et al.Chronic ethanol intake impairs insulin signaling in rats by disrupting Akt association with the cell membrane. Role of TRB3 in inhibition of Akt/protein kinase B activation[J].J Biol Chem,2006,281(16):11126

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备注/Memo

备注/Memo:
*河南省医学科技攻关计划重点项目201002010 #通讯作者,女,1956年2月生,博士,教授,主任医师,研究方向:妊娠期糖尿病胰岛素抵抗的相关因素,Email:zhangsuhe@126.com
更新日期/Last Update: 2014-04-23