[1]苏利沙),张根豪),邓少丽),等.常用免疫抑制剂治疗对系统性红斑狼疮患者淋巴细胞亚群的影响*[J].郑州大学学报(医学版),2014,(02):236.
 SU Lisha,ZHANG Genhao,DENG Shaoli,et al.Changes of lymphocyte subsets in systemic lupus erythematosus patients after common immunosuppressant therapy[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(02):236.
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常用免疫抑制剂治疗对系统性红斑狼疮患者淋巴细胞亚群的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年02期
页码:
236
栏目:
应用研究
出版日期:
2014-03-20

文章信息/Info

Title:
Changes of lymphocyte subsets in systemic lupus erythematosus patients after common immunosuppressant therapy
作者:
苏利沙1)张根豪1)邓少丽2)张俊华1)刘红春1)#
1)郑州大学第一附属医院检验科 郑州 450052 2)第三军医大学大坪医院检验科 重庆 400042
Author(s):
SU Lisha1)ZHANG Genhao1)DENG Shaoli2)ZHANG Junhua1)LIU Hongchun1)
1)Clinical Laboratory, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Clinical Laboratory,Daping Hospital,the Third Military Medical University,Chongqing 400042
关键词:
系统性红斑狼疮淋巴细胞亚群免疫抑制剂
Keywords:
systemic lupus erythematosus lymphocyte subset immunosuppressant
分类号:
A
文献标志码:
R446.63
摘要:
目的:探讨常用免疫抑制剂(糖皮质激素联合环磷酰胺或吗替麦考酚酯)治疗对系统性红斑狼疮(SLE)患者外周血淋巴细胞亚群影响情况。 方法:利用流式细胞仪检测83例SLE患者(24例未治疗患者和59例经免疫抑制剂治疗患者)及30名健康对照者外周血T细胞、B细胞和NK细胞比例。结果:与对照组比较,SLE组患者CD3+CD8+Ts细胞增加(t=5.552,P<0.001),CD3+CD4+Th细胞减少(t=8.061,P<0.001),Th/Ts比值降低(Z=6.880,P<0.001),CD3-CD16+CD56+NK细胞减少(Z=3.637,P<0.001),CD3-CD19+B细胞增加(Z=2.500,P=0.012)。同未治疗组相比,治疗组患者CD3+T细胞有增加趋势,但差异无统计学意义(t=1.802,P=0.075),CD3+CD4+Th细胞升高(t=2.581,P=0.012),Th/Ts比值升高(Z=2.205,P=0.027),CD3-CD19+B细胞降低(Z=2.151,P=0.031)。结论:淋巴细胞亚群比例失调在SLE患者中普遍存在,可能参与了SLE的发病机制;联合应用糖皮质激素和环磷酰胺或吗替麦考酚酯可有效调节Th/Ts比例,选择性抑制B细胞,使SLE患者外周血淋巴细胞各亚群比例趋于平衡。
Abstract:
Aim: To investigate the changes of peripheral blood lymphocyte subsets in systemic lupus erythemaosus(SLE) patients with therapy of common immunosuppressant (prednisolone and CTX or MMF). Methods: The lymphocyte subsets of 83 patients with SLE (24 without therapy and 59 with therapy) and 30 healthy controls were chosen and the proportion of T cells, B cells and NK cells in peripheral blood was measured with flow cytometry. Results: The percentage of CD3+CD4+Th cells(t=8.061,P<0.001), CD3-CD16+CD56+NK cells(Z=3.637,P<0.001) and the Th/Ts ratio(Z=6.880,P<0.001) were significantly lower in SLE patients than those in controls, while CD3+CD8+ Ts cells(t=5.552,P<0.001) and CD3-CD19+B cells(Z=2.500,P=0.012) were higher than those in controls. Compared with those without therapy, CD3+T cells in patients with therapy increased, but the change was not obvious(t=1.802,P=0.075). In comparison, the CD3+CD4+ Th cells increased significantly(t=2.581,P=0.012), so did the Th/Ts ratio(Z=2.205,P=0.027). CD3-CD19+B cells in those with therapy were obviously fewer than those without therapy(Z=2.151,P=0.031). Conclusion: The imbalance of lymphocyte subsets is common in SLE patients, which could involve in the pathogenesis of SLE. Compared with those without therapy, the combined therapy of prednisolone and CTX or MMF could regulate the Th/Ts ratio effectively and suppress B cells selectively, by which they contribute to the balance of lymphocyte subsets in SLE patients.

参考文献/References:

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备注/Memo

备注/Memo:
*国家自然科学基金81101199;河南省教育厅自然科学基金资助项目12A320035 #通讯作者,女,1963年1月生,博士,教授,主任技师,研究方向:自身免疫性疾病实验诊断,Email:xingyunerliu@163.com
更新日期/Last Update: 2014-04-23