[1]戴翠萍)△,张徐宁),薛彩萍),等.5羟基喜树碱脂质体与顺铂联用对耐顺铂的人食管癌Eca109细胞周期和凋亡的影响*[J].郑州大学学报(医学版),2014,(03):301.
 DAI Cuiping),ZHANG Xuning),XUE Caiping),et al.Effects of hydroxycamptothecin liposome combined with cisplatin on cell cycle and apoptosis of cisplatinresistanted human esophageal carcinoma Eca109 cell[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(03):301.
点击复制

5羟基喜树碱脂质体与顺铂联用对耐顺铂的人食管癌Eca109细胞周期和凋亡的影响*
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年03期
页码:
301
栏目:
食管癌研究
出版日期:
2014-05-20

文章信息/Info

Title:
Effects of hydroxycamptothecin liposome combined with cisplatin on cell cycle and apoptosis of cisplatinresistanted human esophageal carcinoma Eca109 cell
作者:
戴翠萍1)△张徐宁1)薛彩萍1)刘家秀1)张玉领1)杨学艺1)陈文中2)季宁东1)
1)淮阴卫生高等职业技术学校淮安市消化道肿瘤重点实验室 淮安 2233002)南京思科药业有限公司 南京 210061
Author(s):
DAI Cuiping1)ZHANG Xuning1)XUE Caiping1)LIU Jiaxiu1)ZHANG Yuling1)YANG Xueyi1)CHEN Wenzhong2)JI Ningdong1)
1)Huai an Key Laboratory of Tumor in Digestive System,Huaiyin Advanced Vocational and Technical Health School,Huaian 2233002)Nanjing Sike Medical Co. Ltd.,Nanjing 210061
关键词:
羟基喜树碱脂质体顺铂食管癌细胞周期细胞凋亡
Keywords:
Stathmin paclitaxel EC9706 cell esophageal squamous cell carcinoma
分类号:
R735.1
摘要:
摘要目的:研究羟基喜树碱脂质体(LHCPT)与顺铂(DDP)联用对耐DDP的人食管癌细胞Eca109细胞(Eca109/DDP)细胞周期和凋亡的影响。方法:取对数生长期的Eca109/DDP细胞,分别用0、1 mg/L DDP处理,再用0.00、0.49、0.98、1.96、3.92、7.84 μg/L LHCPT处理72 h,MTT法测定细胞增殖状况。取对数生长期的Eca109/DDP细胞,分别用0、1 mg/L DDP处理后,再用0.00、3.92 μg/L LHCPT处理72 h,用流式细胞仪检测细胞周期分布及细胞凋亡情况。结果:相对于单独应用,LHCPT与DDP联合应用抑制了Eca109/DDP细胞的增殖(FLHCPT=40.330,FDDP=641.010,F交互=20.330,P均<0.001),以3.92 μg/L LHCPT与1 mg/L DDP联用效果最佳(P<0.05)。相对于单独应用,3.92 μg/L LHCPT与1 mg/L DDP联用可阻滞Eca109/DDP细胞在G0/G1期(FLHCPT=16.184,FDDP=33.660,F交互=15.100,P均<0.05),同时增加细胞的早期凋亡率、晚期凋亡率和总凋亡率(P均<0.05),二者具有协同效应。结论:LHCPT具有辅助DDP治疗食管癌的作用。
Abstract:
AbstractAim: To investigate the effects of hydroxycamptothecin liposome(LHCPT) combined with cisplatin(DDP) on cell cycle and apoptosis of DDPresistanted human esophageal carcinoma cell Eca109(Eca109/DDP).Methods:Eca109/DDP cells were treated with 0,1 mg/L DDP,and then treated with 0.00,0.49,0.98,1.96,3.92,7.84 μg/L LHCPT for 72 h, respectively;MTT method was applicated to detect the cell proliferation.Eca109/DDP cells were treated with 0,1 mg/L DDP,and then treated with 0.00,3.92 μg/L LHCPT for 72 h, respectively;flow cytometry was used to study the cell cycle and apoptosis.Results:Compared with single drug treatment, the growth inhibition effect on Eca109/DDP cells in LHCPT+DDP group was significant(FLHCPT=40.330,FDDP=641.010,Finteraction=20.330,P<0.001),especially 3.92 μg/L LHCPT+1 mg/L DDP.Compared with single drug treatment, 3.92 μg/L LHCPT+1 mg/L DDP could significantly block Eca109/DDP cells at G0/G1 phase(FLHCPT=16.184,FDDP=33.660,Finteraction=15.100,P<0.05), and increase the early apoptotic rate,late apoptotic rate and total apoptotic rate(P<0.05).Conclusion: LHCPT has the potential to be an adjunct to chemotherapy for esophageal carcinoma.

参考文献/References:

参考文献

相似文献/References:

[1]周云)△,王晚萍).人重组p53腺病毒感染p53突变mBGC823细胞对顺铂敏感性的影响*[J].郑州大学学报(医学版),2009,(05):948.
 ZHOU Yun),WANG Wanping).Adenovirus mediated p53 gene enhances the sensitivity of gastric cancer cells containing mutanttype p53 to cisplatin[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):948.
[2]周云△.顺铂和5氟尿嘧啶联合替尼泊苷或依托泊甙治疗晚期胃癌疗效对比*[J].郑州大学学报(医学版),2009,(05):955.
 ZHOU Yun.Comparison of regimen VCF and ECF in treatment of advanced gastric cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):955.
[3]常方方,高明,周云#.奥沙利铂联合顺铂对体外培养BGC823细胞增殖及Bcl2、Bax、Caspase3蛋白表达的影响[J].郑州大学学报(医学版),2009,(02):359.
 CHANG Fangfang,GAO Ming,ZHOU Yun.Effects of OXA and DDP on cell proliferation and Bcl2,Bax,Caspase3 expression of BGC823 cells in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):359.
[4]韩萍)△,赵妍娟),裴兰英),等.顺铂联合葛根素纯品和葛根粗提物对H446细胞增殖及Bax、Bcl2蛋白表达的影响[J].郑州大学学报(医学版),2010,(03):477.
 HAN Ping),ZHAO Yanjuan),PEI Lanying),et al.Effects of cisplatin combined with pure puerarin or pueraria crude extract on proliferation,expressions of Bax and Bcl2 of H446 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(03):477.
[5]吕品田),檀碧波),刘斌),等.胃癌淋巴结转移灶对顺铂体外化疗的药敏性及多种耐药相关因子的表达[J].郑州大学学报(医学版),2010,(04):566.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(03):566.
[6]郝秀轻,张林西,孙黎,等.NS398联合顺铂对Eca109细胞增殖及凋亡的影响[J].郑州大学学报(医学版),2010,(05):713.
 HAO Xiuqing,ZHANG Linxi,SUN Li,et al.Effects of NS398 combined with cisplatin on apoptosis and proliferation of Eca109 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(03):713.
[7]韩娜,王健,张中冕.培美曲塞联合顺铂治疗晚期非小细胞肺癌18例[J].郑州大学学报(医学版),2010,(05):876.
[8]江亚南),马俊芬),黄幼田),等.顺铂对TRAIL诱导EC1细胞凋亡的增敏效应*[J].郑州大学学报(医学版),2011,(03):346.
 JIANG Yanan),MA Junfen),HUANG Youtian),et al.Sensitive enhancement of cisplatin on TRAILmediated apoptosis in EC1 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(03):346.
[9]饶玉梅)△,陈刚),李留霞),等.miR106b对HeLa细胞顺铂化疗敏感性的影响*[J].郑州大学学报(医学版),2013,(05):637.
 RAO Yumei),CHEN Gang),LI Liuxia),et al.Effects of miR106b on cisplatin chemosensitivity in HeLa cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(03):637.

备注/Memo

备注/Memo:
*江苏省卫生职业教育研究指导性项目JZ200911;淮安市科技支撑计划项目HAS2009002△女,1972年10月生,硕士,副教授,研究方向:消化道肿瘤,Email:38224722@qq.com
更新日期/Last Update: 1900-01-01