[1]金小小),张淑玲),徐黎娟),等.ClC-3在SIN1诱导的大鼠海马神经元凋亡中的作用*[J].郑州大学学报(医学版),2014,(03):315.
 JIN Xiaoxiao,ZHANG Shuling),XU Lijuan),et al.Effect of ClC3 on hippocampal neuronal apoptosis of rats induced by SIN1[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(03):315.
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ClC-3在SIN1诱导的大鼠海马神经元凋亡中的作用*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年03期
页码:
315
栏目:
论著
出版日期:
2014-05-20

文章信息/Info

Title:
Effect of ClC3 on hippocampal neuronal apoptosis of rats induced by SIN1
作者:
金小小1张淑玲2徐黎娟3范洪领1钟志超1李曦1常全忠1)#
1)遵义医学院珠海校区生理学教研室 珠海 5190412)遵义医学院珠海校区儿科学教研室 珠海 5190413)莆田市第一医院检验科 莆田 351100
Author(s):
JIN Xiaoxiao1 ZHANG Shuling2) XU Lijuan3) FAN Hongling1 ZHONG Zhichao1 LI Xi1 CHANG Quanzhong1
1)Department of Physiology,Zhuhai Campus of Zunyi Medical College,Zhuhai 5190412)Department of Pediatrics, Zhuhai Campus of Zunyi Medical College,Zhuhai 5190413)Department of Clinical Laboratory,the First Hospital of Putian City,Putian 351100
关键词:
ClC3氯通道3吗啡斯得酮胺海马神经元细胞凋亡44二异硫氰基芪22二磺酸
Keywords:
ClC3 chloride channelSIN1hippocampal neuroncell apoptosisDIDS
分类号:
R332
摘要:
摘要目的:探讨ClC3氯通道在NO供体SIN1诱导的大鼠海马神经元凋亡中的作用。方法:取离体培养12 d的SD大鼠海马神经元,分为对照组、SIN1组和SIN1+DIDS组。对照组不作处理,SIN1组加入终浓度为0.5 mmol/L SIN1作用18 h,SIN1+DIDS组加入终浓度为0.5 mmol/L SIN1和0.1 mmol/L DIDS作用18 h。采用细胞免疫荧光染色和免疫印迹技术检测各组ClC3氯通道蛋白的表达,实时定量PCR技术检测各组ClC3氯通道 mRNA的表达。结果:各组Caspase3和ClC3氯通道表达的差异有统计学意义(蛋白:FCaspase3=118.330,P<0.001;FClC3氯通道=102.750,P<0.001;mRNA:FClC3氯通道=32.955,P<0.001)。SIN1诱导凋亡神经元ClC3氯通道蛋白表达增强,氯通道阻断剂DIDS削弱ClC3氯通道的表达。结论:ClC3氯通道可能参与SIN1诱导大鼠海马神经元的凋亡。
Abstract:
AbstractAim: To explore the expression of ClC3 in the hippocampal neuronal apoptosis of rats induced by SIN1. Methods: The hippocampal neurons from SD rats cultured for 12 days were randomly allocated into control group,SIN1 group and SIN1+DIDS group. The neurons in control group were not treated;the neurons in SIN1 group were treated with 0.5 mmol/L SIN1 for 18 h,and the neurons in SIN1+DIDS group were treated with 0.5 mmol/L SIN1 and 0.1 mmol/L DIDS for 18 h.Immunofluorescent staining, Western blot and PCR method were used to detect the expression of ClC3 chloride. Results: The expressions of ClC3 and Caspase3 were different among the 3 groups(protein: FCaspase3= 118.330,P<0.001; FClC3=102.750,P<0.001;mRNA:FClC3=32.955,P<0.001). The expression of ClC3 was enhanced after the SIN1 induce neuronal apoptosis. Chloride channel blocker DIDS could significantly weaken the expression of ClC3 inducing by SIN1.Conclusion: ClC3 may participate in the hippocampal neuron apoptosis induced by SIN1.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目81160157;贵州省优秀科技人才省长基金201209 #通讯作者,男,1965年5月生,博士,教授,研究方向:缺血性脑损伤及其保护机制,Email:cqzchang@sina.com
更新日期/Last Update: 1900-01-01