[1]冯悦静),魏小玲),尤爱国),等.肺癌患者血清中DNMT1、DNMT3a、DNMT3b和HDAC1蛋白的表达*[J].郑州大学学报(医学版),2014,(03):323.
 FENG Yuejing,WEI Xiaoling,YOU Aiguo,et al.Expressions of DNMT1,DNMT3a,DNMT3b and HDAC1 in serum from patients with lung cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(03):323.
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肺癌患者血清中DNMT1、DNMT3a、DNMT3b和HDAC1蛋白的表达*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年03期
页码:
323
栏目:
论著
出版日期:
2014-05-20

文章信息/Info

Title:
Expressions of DNMT1,DNMT3a,DNMT3b and HDAC1 in serum from patients with lung cancer
作者:
冯悦静1魏小玲2尤爱国3周安燕1吴拥军2王威2何其栋2王静1)#
1)郑州大学第一附属医院呼吸内科 郑州 4500522)郑州大学公共卫生学院毒理学教研室 郑州 4500013)河南省疾病预防控制中心传染病预防控制所 郑州 450016
Author(s):
FENG Yuejing1WEI Xiaoling2YOU Aiguo3ZHOU Anyan1WU Yongjun2WANG Wei2HE Qidong2WANG Jing1
1)Department of Respiratory Disease,the First Affiliated Hospital, Zhengzhou University,Zhengzhou 4500522)Department of Toxicology,College of Public Health,Zhengzhou University,Zhengzhou 450001 3)Institute of Infectious Disease Control and Prevention, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016
关键词:
肿瘤DNA甲基化转移酶组蛋白去乙酰化酶1
Keywords:
lungneoplasmDNA methyltransferasehistone deacetylases 1
分类号:
R734.2
摘要:
摘要目的:研究肺癌患者血清中DNA甲基化转移酶(DNMT)1、DNMT3a、DNMT3b和组蛋白去乙酰化酶1(HDAC1)蛋白的表达情况。方法:采用酶联免疫吸附试验(ELISA)方法测定136例肺癌患者、140例肺良性疾病及145例健康体检者血清中DNMT1、DNMT3a、DNMT3b和HDAC1蛋白的表达,并分析DNMT1、DNMT3a、DNMT3b和HDAC1蛋白表达与肺癌临床病理特征的关系。结果:3组血清中DNMT1、DNMT3a、DNMT3b和HDAC1蛋白表达差异有统计学意义(F=3.281、62.510、37.273、57.721,P<0.05),肺癌患者均高于正常对照及肺良性疾病患者;非条件logistic回归分析提示DNMT1、DNMT3a、DNMT3b和HDAC1蛋白高表达均可影响肺癌的发生发展(P<0.001);肺癌患者血清中DNMT1、DNMT 3a、DNMT 3b和HDAC1蛋白表达无组织学类型特异性(P>0.05),但不同临床分期其蛋白表达差异有统计学意义(t=0.843、0.244、0.222、0.878,P<0.05)。结论:肺癌患者血清中DNMT1、DNMT3a、DNMT3b和HDAC1蛋白的高表达可能在肺癌的发生发展中发挥一定的作用。
Abstract:
AbstractAim: To study the expressions of DNMT1, DNMT3a, DNMT3b and HDAC1 in serum from patients with lung cancer. Methods: ELISA was used to measure the expressions of DNMT1, DNMT3a, DNMT3b and HDAC1 in the serum from 136 lung cancer patients,140 cases of lung benign diseases and 145 healthy people (control group), and the relationship between the expressions of DNMT1, DNMT3a, DNMT3b, HDAC1 and clinical characteristics of lung cancer was analyzed. Results:The expressions of DNMT1, DNMT3a, DNMT3b and HDAC1 in patients with lung cancer were higher than those in lung benign diseases group and control group(F=3.281,62.510,37.273,57.721,P<0.05). The result of logistic regression showed that the overexpressions of DNMT1, DNMT3a,DNMT3b and HDAC1 might increase the risk of lung cancer (P<0.001). There were no correlations among the expressions of DNMT1, DNMT3a, DNMT3b or HDAC1 between different histological types (P>0.05),while there were statistical significances in the expressions of DNMT1, DNMT3a, DNMT3b and HDAC1 between different stages (t=0.843,0.244,0.222,0.878,P<0.05).Conclusion: The overexpressions of DNMT1, DNMT3a, DNMT3b and HDAC1 could contribute to the occurrence and development of lung cancer.

参考文献/References:

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30972457;河南省重大科技攻关项目112102310102 #通讯作者,女,1958年11月生,博士,教授,研究方向:肺癌的发病机制和早期预警,Email:wangjing@zzu.edu.cn
更新日期/Last Update: 1900-01-01