[1]郭历琛),史惠蓉)#,盛浴澜),等.LRIG3反义寡核苷酸对宫颈癌Hela229细胞LRIG3、EGFR表达及增殖的影响[J].郑州大学学报(医学版),2014,(03):326.
 GUO Lichen,SHI Huirong,SHENG Yulan,et al.Effect of LRIG3targeted antisense oligonucleotides on proliferation and expressions of LRIG3 and EGFR in Hela229 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(03):326.
点击复制

LRIG3反义寡核苷酸对宫颈癌Hela229细胞LRIG3、EGFR表达及增殖的影响
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年03期
页码:
326
栏目:
论著
出版日期:
2014-05-20

文章信息/Info

Title:
Effect of LRIG3targeted antisense oligonucleotides on proliferation and expressions of LRIG3 and EGFR in Hela229 cells
作者:
郭历琛12史惠蓉1)#盛浴澜2张平安2张锐2
1)郑州大学第一附属医院妇产科 郑州 4500522)上海市嘉定区中心医院妇产科 上海 201800
Author(s):
GUO Lichen12 SHI Huirong1 SHENG Yulan2 ZHANG Pingan2 ZHANG Rui2
1)Department of Gynecology and Obstetrics, the First Affiliated Hospital,Zhengzhou University, Zhengzhou 4500522)Department of Gynecology and Obstetrics, Central Hospital of Shanghai Jiading District, Shanghai 201800
关键词:
LRIG3反义寡核苷酸EGFR增殖宫颈癌
Keywords:
LRIG3antisense oligonucleotideEGFRproliferationcervical carcinoma
分类号:
R737.33
摘要:
摘要目的:探讨LRIG3反义寡核苷酸(ASODN)对宫颈鳞癌Hela229细胞LRIG3、EGFR表达及增殖的影响。方法:利用阳离子脂质体介导法分别用150、200及250 mg/L的LRIG3 ASODN、正义寡核苷酸(SODN)及无关序列核苷酸(MSODN)转染Hela229细胞24、48及72 h,分别应用RTPCR和Western blot法检测细胞LRIG3、EGFR mRNA及蛋白的表达情况,应用MTT法检测细胞增殖率。 以常规培养的细胞作空白对照。结果:转染24、48、72 h后,ASODN组细胞增殖率均较空白对照组、SODN组、MSODN组明显增高(P<0.05),LRIG3 mRNA和蛋白表达降低(P<0.05),EGFR mRNA和蛋白表达增高(P<0.05);3个ASODN剂量组间比较,随着LRIG3 ASODN剂量的增加,细胞增殖率增高(P<0.05),LRIG3 mRNA和蛋白表达水平降低(P<0.05),EGFR mRNA和蛋白表达水平升高(P<0.05)。结论:LRIG3 ASODN可促进宫颈鳞癌Hela229细胞的增殖,该效应可能与抑制LRIG3表达、促进EGFR表达有关。
Abstract:
AbstractAim: To observe the proliferation and expressions of LRIG3 and EGFR in Hela229 cells transfected with LRIG3targeted antisense oligonucleotide(ASODN).Methods:The ASODN, SODN and MSODN of LRIG3 at doses of 150, 200 and 250 mg/L were transfected into Hela229 cells by the cationic liposome respectively for 24, 48, and 72 h. RTPCR and Western blot were used to detect the expressions of LRIG3 and EGFR,and MTT assay was used to examine the proliferation of Hela229 cells. The cells with normal incubation were the blank control. Results: Compared with the blank control group, SODN group and MSODN group, the proliferation rate of ASODN group was significantly higher(P<0.05), the expressions of LRIG3 mRNA and protein decreased(P<0.05), and the expressions of EGFR mRNA and protein increased with the increase of ASODN concentration(P<0.05).Conclusion: LRIG3 ASODN can promote the proliferation of Hela229 cells, which is related to inhibition of LRIG3 expression and promotion of EGFR expression.

参考文献/References:

[1]Holmlund C,Nilsson J,Guo D,et al.Characterization and tissuespecific expression of human LRIG2[J].Gene,2004,332:35 [2]Suzuki Y,Sato N,Tohyama M,et al.cDNA cloning of a novel membrane glycoprotein that is expressed specifically in glial cells in the mouse brain. LIG1, a protein with leucinerich repeats and immunoglobulinlike domains[J].J Biol Chem,1996,271(37):22522 [3]Muller S,Lindquist D,Kanter L,et al.Expression of LRIG1 and LRIG3 correlates with human papillomavirus status and patient survival in cervical adenocarcinoma[J].Int J Oncol,2013,42(1):247 [4]Guo D,Holmlund C,Henriksson R,et al.The LRIG gene family has three vertebrate paralogs widely expressed in human and mouse tissues, and a homolog in Ascidiacea[J].Genomics,2004,84(1):157 [5]Yi W,Haapasalo H,Holmlund C,et al.Expression of leucinerich repeats and immunoglobulinlike domains (LRIG) proteins in human ependymoma relates to tumor location, WHO grade, and patient age[J].Clin Neuropathol,2009,28(1):21 [6]Abraira VE,Satoh T,Fekete DM,et al.Vertebrate Lrig3ErbB interactions occur in vitro but are unlikely to play a role in Lrig3dependent inner ear morphogenesis[J].PLoS One,2010,5(2):e8981 [7]Salomon DS,Brandt R,Ciardiello F,et al.Epidermal growth factorrelated peptides and their receptors in human malignancies[J].Crit Rev Oncol Hematol,1995,19(3):183 [8]Citri A,Yarden Y.EGFERBB signalling:towards the systems level[J].Nat Rev Mol Cell Biol,2006,7(7):505 [9]Bhattacharjee A,Richards WG,Staunton J,et al.Classification of human lung carcinomas by mRNA expression profiling reveals distinct adenocarcinoma subclasses[J].Proc Natl Acad Sci USA,2001,98(24):13790 [10]Lapointe J,Li C,Higgins JP,et al.Gene expression profiling identifies clinically relevant subtypes of prostate cancer[J].Proc Natl Acad Sci USA,2004,101(3):811 [11]尤超,温洪涛,关冰.食管鳞状细胞癌组织中LRIG3和EGFR蛋白的表达[J].郑州大学学报:医学版,2012,47(2):147 (20131210收稿责任编辑王曼)

相似文献/References:

[1]亚国伟,张威,陈玉,等.PTEN反义寡核苷酸对EC9706细胞增殖、凋亡和PTEN、mTOR表达的影响*[J].郑州大学学报(医学版),2010,(01):16.
 YA Guowei,ZHANG Wei,CHEN Yu,et al.Changes of proliferation, apoptosis and expression of mTOR and PTEN in EC9706 cells after gene PTEN inhibition by antisense oligonucleotides[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(03):16.
[2]陈奎生,王志永,周强,等.真核起始因子4E反义寡核苷酸对人食管癌EC1细胞中真核起始因子4E表达的影响*[J].郑州大学学报(医学版),2009,(05):935.
 CHEN Kuisheng,WANG Zhiyong,ZHOU Qiang,et al.Effect of antisense oligodeoxynucleotides of eukaryotic initiation factor 4E on eIF4E expression in human esophageal carcinoma EC1 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):935.
[3]权松霞),张振中),邵彦江),等.脂质体介导的hTERT PEI/ASODN缩合体对乳癌MCF7细胞生长及hTERT表达的影响[J].郑州大学学报(医学版),2010,(04):554.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(03):554.
[4]张岚),高冬玲),温洪涛),等.BclXL反义寡核苷酸对食管癌EC9706细胞和裸鼠移植瘤组织的诱凋亡作用*[J].郑州大学学报(医学版),2014,(02):146.
 ZHANG Lan),GAO Dongling),WEN Hongtao),et al.Induced effects of apoptosis on EC9706 cells and xenograft tissue by BclXL antisense oligodeoxynucleotide[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(03):146.

备注/Memo

备注/Memo:
#通讯作者,女,1959年12月生,博士,教授,主任医师,研究方向:妇科肿瘤,Email:Hrshi2011@163.com
更新日期/Last Update: 1900-01-01