[1]张继新,庞志刚#,刘超,等.阿霉素、5氟尿嘧啶联合热疗对SMMC7721细胞增殖及凋亡的影响[J].郑州大学学报(医学版),2014,(03):365.
 ZHANG Jixin,PANG Zhigang,LIU Chao,et al.Effect of adriamycin and 5fluorouracil combined with hyperthermia on proliferation and apoptosis of SMMC7721 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(03):365.
点击复制

阿霉素、5氟尿嘧啶联合热疗对SMMC7721细胞增殖及凋亡的影响
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年03期
页码:
365
栏目:
应用研究
出版日期:
2014-05-20

文章信息/Info

Title:
Effect of adriamycin and 5fluorouracil combined with hyperthermia on proliferation and apoptosis of SMMC7721 cells
作者:
张继新庞志刚#刘超刘新江尚闯耿翔
郑州大学第二附属医院普外科 郑州 450014
Author(s):
ZHANG Jixin PANG Zhigang LIU Chao LIU Xinjiang SHANG ChuangGENG Xiang
Department of General Surgery,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014
关键词:
阿霉素5氟尿嘧啶热疗热化疗SMMC7721细胞细胞增殖细胞凋亡
Keywords:
AbstractAim: To study the effect of adriamycin(ADM) and 5fluorouracil(5FU) combined with hyperthermia on proliferation and apoptosis of SMMC7721 cells. Methods: SMMC7721 cells were allocated into 6 groups:control grouppure hyperthermia group 5FU+AD
分类号:
R735.7
摘要:
摘要目的:探讨阿霉素(ADM)、5氟尿嘧啶(5FU)联合热疗对肝癌SMMC7721细胞增殖和凋亡的影响。方法:SMMC7721细胞分为对照组、单纯热疗组、5FU+ADM组、ADM+热疗组、5FU+热疗组、5FU+ADM+热疗组,各组热处理均为43 ℃水浴1 h再37 ℃培养23 h。各组处理24 h后采用MTT法检测细胞的增殖,计算增殖抑制率,采用流式细胞术检测细胞凋亡,计算凋亡率。结果:5FU+ADM+热疗组细胞增殖抑制率高于单纯热疗组、5FU+ADM组、ADM+热疗组、5FU+热疗组[(74.66±1.61)% vs (19.76±1.41)%、(55.04±1.89)%、(40.21±2.27)%、(35.56±2.24)%,(F=76.843,P<0.001)]。5FU+ADM+热疗组的细胞凋亡率高于单纯热疗组、5FU+ADM组、ADM+热疗组、5FU+热疗组[(80.13±2.42)% vs (28.38±1.92)%、(65.40±2.05)%、(49.32±1.76)%、(45.99±1.70)%,(F=230.506,P<0.001)]。结论:加热能增强5FU联合ADM化疗对SMMC7721细胞增殖的抑制和凋亡诱导作用,其效应优于单纯热疗或ADM与5FU联合以及单纯化疗联合热疗。
Abstract:
adriamycin;5fluorouracil;hyperthermia;thermochemotherapy;SMMC7721 cell;cell proliferation;cell apoptosis

参考文献/References:

[1]Hiraoka M, Masunaga S, Nishimura Y, et al. Regional hyperthermia combined with radiotherapy in the treatment of lung cancers[J].Int J Radiat Oncol Biol Phys,1992,22(5):1009 [2]何跃明,吕新生,艾中立,等.磁介导热疗联合顺铂化疗对肝癌细胞的影响及其机制[J].中华实验外科杂志,2004,21(10):1170 [3]徐春燕,高姗,王琳,等.不同温度热疗联合紫杉醇化疗对肺癌A549细胞增殖及Akt蛋白表达的影响[J].郑州大学学报:医学版,2008,43(3):524 [4]王倩荣,史正华,马骥,等.阿霉素热化疗对肝癌细胞增殖和凋亡的影响[J].现代肿瘤医学,2011,19(10):1918 [5]刘贤英,徐茂凤,金春香,等.5氟尿嘧啶联合热疗对人肝癌细胞株的增殖抑制作用[J].中国综合临床,2010,26(12):1233 [6]Gillies RJ,Gatenby RA.Hypoxia and adaptive landscapes in the evolution of carcinogenesis[J].Cancer Metastasis Rev,2007,26(2):311 [7]Raut CP,Evans DB,Crane CH,et al.Neoadjuvant therapy for respectable pancreatic cancer[J].Surg Oncol Clin N Am,2004,13(4):639 [8]Sakaguchi Y,Stephens LC,Makino M,et al.Apoptosis in tumors and normal tissues induced by whole body hyperthermia in rats[J].Cancer Res,1995,55(22):5459 [9]Hildebrandt B,Wust P,Ahlers O,et al.The cellular and molecular basis of hyperthermia[J].Crit Rev Oncol Hematol,2002,43(1):33 [10]张洪新,王执民,郭卫平,等.丝裂霉素C热化疗对人肝癌细胞7721的细胞毒作用[J].第四军医大学学报,1998(6):636 [11]孙跃民,傅军,张伟,等.温热化疗对胃、结肠癌细胞的体外作用研究[J].癌症,2006,25(8):974 [12]Westermann AM,Grosen EA,Katschinski DM,et al.A pilot study of whole body hyperthermia and carboplatin in platinumresistant ovarian cancer[J].Eur J Cancer,2001,37(9):1111 [13]Nikfarjam M,Muralidharan V,MalcontentiWilson C,et al.The apoptotic response of liver and colorectal liver metastases to focal hyperthermic injury[J].Anticancer Res,2005,25(2B):1413 [14]EllingerZiegelbauer H,Karasuyama H,Yamada E,et al.Ste20like kinase(SLK), a regulatory kinase for pololike kinase(Plk) during the G2/M transition in somatic cells[J].Genes Cells,2000,5(6):491 [15]Sun X,Xing L,Ling CC,et al.The effect of mild temperature hyperthermia on tumor hypoxia and blood perfusion:relevance for radiotherapy,vascular targeting and imaging[J].Int J Hyperthermia,2010,26(3):224 [16]Kim YJ,Yoon SY,Kim JT,et al.NDRG2 suppresses cell proliferation through downregulation of AP1 activity in human colon carcinoma cells[J].Int J Cancer,2009,124(1):7 [17]刘宝瑞,钱晓萍.肿瘤热化疗的基础与临床研究进展[J].国外医学:肿瘤学分册,2004,31(1):34 [18]Burd R,Dziedzic TS,Xu Y,et al.Tumor cell apoptosis, lymphocyte recruitment and tumor vascular changes are induced by low temperature, long duration(feverlike) whole body hyperthermia[J].J Cell Physiol,1998,177(1):137 [19]Hsuen Lim E,Seng Cheong Lim R,Seng Wu T,et al.Oxaliplatin/fluorouracil/leucovorin in advanced colorectal carcinoma: an asian experience[J].Ann Pharmacother,2003,37(12):1909

相似文献/References:

[1]李珊珊),罗忠民),崔会娟),等.全反式维甲酸联合5氟尿嘧啶对Eca109细胞增殖、迁移和VEGF表达的影响*[J].郑州大学学报(医学版),2015,(01):1.
 LI Shanshan,LUO Zhongmin,CUI Huijuan,et al.Effects of alltrans retinoic acid combined with 5fluorouracil on proliferation, migration, and VEGF expression of Eca109 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(03):1.
[2]杨德彬),路明)#.川芎嗪对阿霉素诱导的大鼠心肌重塑中TGFβ1表达的影响*[J].郑州大学学报(医学版),2015,(01):71.
 YANG Debin),LU Ming).Effect of ligustrazine on myocardial remodeling in rats with adriamycin induced expression of TGFβ1[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(03):71.
[3]贾 欣,冯倩华,张超锋,等.透明质酸修饰的中空介孔硫化铜负载阿霉素载药体系在乳腺癌模型裸鼠体内的分布行为及抗肿瘤效果观察[J].郑州大学学报(医学版),2018,(06):738.[doi:10.13705/j.issn.1671-6825.2018.05.025]
 JIA Xin,FENG Qianhua,ZHANG Chaofeng,et al.Biodistribution and pharmacodynamics of drug delivery system based on hyaluronic acid-modified doxorubicin-loaded hollow mesoporous copper sulfide nanoparticles in breast tumor bearing mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(03):738.[doi:10.13705/j.issn.1671-6825.2018.05.025]

备注/Memo

备注/Memo:
#通讯作者,男,1963年7月生,硕士,主任医师,教授,研究方向:原发性肝癌,Email:pzg63726@sina.com
更新日期/Last Update: 1900-01-01