[1]管一春),杨雪峰)#,王兴玲),等.睾酮对子宫内膜腺癌细胞LIF、HOXA10蛋白表达的影响*[J].郑州大学学报(医学版),2014,(03):370.
 GUAN Yichun,YANG Xuefeng,WANG Xingling,et al.Effect of testosterone on expressions of leukemia inhibitor factor and Homeobox gene A10 in endometrial adenocarcinoma cell[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(03):370.
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睾酮对子宫内膜腺癌细胞LIF、HOXA10蛋白表达的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年03期
页码:
370
栏目:
应用研究
出版日期:
2014-05-20

文章信息/Info

Title:
Effect of testosterone on expressions of leukemia inhibitor factor and Homeobox gene A10 in endometrial adenocarcinoma cell
作者:
管一春1杨雪峰2)#王兴玲1孙丽君1张彩霞1李巍巍1娄华1李丽3
1)郑州大学第三附属医院生殖医学中心 郑州 4500522)郑州大学第三附属医院妇产科 郑州 4500523)郑州大学第三附属医院超声科 郑州 450052
Author(s):
GUAN Yichun1 YANG Xuefeng2 WANG Xingling1 SUN Lijun1 ZHANG Caixia1 LI Weiwei1 LOU Hua1 LI Li3
1)Department of Reproduction Center, the Third Affiliated Hospital, Zhengzhou University, Zhengzhou 4500522)Department of Obstetrics and Gynecology, the Third Affiliated Hospital, Zhengzhou University, Zhengzhou 4500523)Department of Ultrasonography, the Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
多囊卵巢综合征白血病抑制因子同源框基因A10Ishikawa细胞睾酮
Keywords:
AbstractAim: To observe the effect of testosterone on expressions of the leukemia inhibitor factor(LIF) and homeobox gene(HOX) A10 in human endometrial adenocarcinoma cell line (Ishikawa). Methods: Ishikawa cells were treated with 0(control)10-810-610
分类号:
R711.7
摘要:
摘要目的:探讨睾酮对子宫内膜腺癌Ishikawa细胞白血病抑制因子(LIF)和同源框基因(HOXA10)表达的影响。方法:培养Ishikawa细胞,分别加入终浓度为0(对照)、10-8、10-6、10-4 mol/L的睾酮溶液,采用免疫细胞化学法检测24和48 h后Ishikawa细胞LIF和HOXA10蛋白的表达。结果:睾酮呈时间和浓度依赖性地性抑制Ishikawa细胞LIF和HOXA10的表达(LIF:F组间=19.205,F时间=32.745,F交互=37.379,P均<0.001。HOXA10:F组间=27.703,F时间=15.379,F交互=22.021,P均<0.001)。结论:高雄激素血症可能是影响多囊卵巢综合征子宫内膜容受性的因素之一。
Abstract:
polycystic ovary syndrome;leukemia inhibitor factor;homeobox gene A10;Ishikawa cell;testosterone

参考文献/References:

[1]Taylor H.The role of HOX genes in human implantation[J]. Hum Reprod Update,2000,6(1):75 [2]Chau MY,Pando S,Taylor H.HOXA11 silencing and endogenous HOXA11 antisense ribonucleic acid in the uterine endometrium[J].J Clin Endocrinol Metab,2002,87(6):2674 [3]魏兆莲,李美芝,陈咏健.高雄激素血症对子宫内膜功能的影响[J].中国妇产科临床杂志,2002,3(3):145 [4]Ito K,Suzuki T,Akahira J,et al.Expression of androgen receptor and 5 alphareductases in the human normal endometrium and its disorders[J].Int J Cancer,2002,99(5):652 [5]Okon MA,Laird SM,Tuckerman EM,et al.Serum androgen levels in women who have recurrent miscarriages and their correlation with markers of endometrial function[J].Fertil Steril,1998,69(4):682 [6]Tuckerman EM,Okon MA,Li T,et al.Do androgens have a direct effect on endometrial function?An in vitro study[J].Fertil Steril,2000,74(4):771 [7]Cermik D,Selam B,Taylor HS.Regulation of HOXA10 expression by testosterone in vitro and in the endometrium of patients with polycystic ovary syndrome[J].J Clin Endocrinol Metab,2003,88(1):238 [8]Apparao KB,Lovely LP,Gui YT,et al.Elevated endometrial androgen receptor expression in women with polycystic ovarian syndrome[J].Biol Reprod,2002,66(2):297 [9]Zhou J,Shen K,Zeng JF,et al.Differential expression of microRNAs associated with estrogen receptor alpha and progesterone receptor in typeⅠ and typeⅡ endometrial adenocarcinomas[J].Zhonghua Fu Chan Ke Za Zhi,2009,44(10):765

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备注/Memo

备注/Memo:
*河南省卫生厅科技攻关项目2011020054#通讯作者,女,1956年5月生,教授,主任医师,研究方向:生殖内分泌及宫颈疾病,Email:yxfeng@zzu.edu.cn
更新日期/Last Update: 1900-01-01