[1]王瑞莉),李庆华),张彦婷),等.DZNep对Eca109细胞增殖、凋亡及迁移能力的影响*[J].郑州大学学报(医学版),2014,(04):449.
 WANG Ruili,LI Qinghua,ZHANG Yanting,et al.Effects of DZNep on proliferation, apoptosis and migration of Eca109 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(04):449.
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DZNep对Eca109细胞增殖、凋亡及迁移能力的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年04期
页码:
449
栏目:
食管癌研究
出版日期:
2014-07-20

文章信息/Info

Title:
Effects of DZNep on proliferation, apoptosis and migration of Eca109 cells
作者:
王瑞莉1)李庆华1)张彦婷1)毛丽红1)蒋海丽1)龚方华2)王静1)薛乐勋12)#关方霞1)#
1)郑州大学生命科学学院 郑州 4500012)郑州大学第一附属医院细胞生物学研究室 郑州 450052
Author(s):
WANG Ruili1) LI Qinghua1) ZHANG Yanting1) MAO Lihong1) JIANG Haili1) GONG Fanghua2) WANG Jing1) XUE Lexun12) GUAN Fangxia1)
1)  College of Life Sciences, Zhengzhou University, Zhengzhou 4500012)Laboratory for Cell Biology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
DZNepEca109细胞增殖凋亡迁移
Keywords:
DZNep Eca109 cell proliferation apoptosis migration
分类号:
R735.1
摘要:
目的:探讨DZNep处理食管鳞状细胞癌Eca109细胞后对其增殖、凋亡及迁移能力的影响。方法:DZNep处理Eca109细胞48 h,以DMSO处理48 h的Eca109细胞作为对照。采用EdU荧光显微镜法检测细胞增殖率,流式细胞仪检测细胞凋亡率,Transwell小室检测细胞迁移数。结果:DZNep处理后的Eca109细胞增殖率为(34.60±4.45)%,低于对照组的(42.37±4.64)%(t=3.625,P=0.002);其细胞凋亡率为(16.27±3.70)%,高于对照组的(7.38±0.77)%(t=7.061,P<0.001);迁移到下室的细胞数为(179±38)个,较对照组的(494±99)个少(t=8.912,P<0.001)。结论:DZNep能够抑制Eca109细胞的增殖和迁移,促进其凋亡。
Abstract:
Aim: To investigate the effect of DZNep on the proliferation, apoptosis and migration of Eca109 cells. Methods: DZNep was added to the medium of Eca109 cells and then incubated for 48 h, meanwhile, DMSO was used as control. The proliferation rate of Eca109 cells was examined by EdU method, the cell apoptosis rate was detected by flow cytometry assay, and cell migration rate was measured using Transwell assay. Results: Eca109 cells treated with DZNep had a lower cell proliferation rate [(34.60±4.45)%] than that of control group [(42.37±4.64)%](t=3.625,P=0.002). Eca109 cells treated with DZNep had a higher cell apoptosis rate [(16.27±3.70)%] than that of control group [(7.38±0.77)%](t=7.061,P<0.001). Eca109 cells treated with DZNep had a lower cell migration number [(179±38) cells] than that of control group [(494±99) cells](t=8.912,P<0.001). Conclusion: DZNep could inhibit the proliferative and migratory ability of Eca109 cells and promote its apoptotic ability.

参考文献/References:

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30700140;科技部国际科技合作基金资助项目2007DFA01240#通讯作者:薛乐勋,男,1944年2月生,本科,教授,研究方向:肿瘤标志物与基因工程,Email:xuelx@zzu.edu.cn;关方霞,女,1969年2月生,博士,教授,研究方向:干细胞与再生医学,Email:guanfangxia@126.com
更新日期/Last Update: 1900-01-01