[1]孙丽娜),王颖)#,燕树勋),等.睾酮对血管紧张素Ⅱ诱导的乳鼠心肌成纤维细胞增殖和胶原合成的影响*[J].郑州大学学报(医学版),2014,(04):461.
 SUN Lina),WANG Ying),YAN Shuxun),et al.Effect of testosterone on proliferation and collagen synthesis of neonatal rat cardiac fibroblast induced by angiotensinⅡ[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(04):461.
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睾酮对血管紧张素Ⅱ诱导的乳鼠心肌成纤维细胞增殖和胶原合成的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年04期
页码:
461
栏目:
论著
出版日期:
2014-07-20

文章信息/Info

Title:
Effect of testosterone on proliferation and collagen synthesis of neonatal rat cardiac fibroblast induced by angiotensinⅡ
作者:
孙丽娜1) 王颖1)# 燕树勋2)张彦周3)魏子涵1) 秦鹏1) 孙素珂4)
1)郑州大学第一附属医院老年医学科 郑州 4500522)河南中医学院第一附属医院内分泌科 郑州 4500083)郑州大学第一附属医院心内科 郑州 4500524)河南省高等学校临床医学重点学科开放实验室 郑州 450052
Author(s):
SUN Lina1)WANG Ying1)YAN Shuxun2)ZHANG Yanzhou3)WEI Zihan1)QIN Peng1)SUN Suke4)
1)Department of Geriatrics,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 4500522)Department of Endocrinology and Metabolism,the First Affiliated Hospital,Henan College of Traditional Chinese Medicine,Zhengzhou 4500083)Department of Cardiology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 4500524)Institute of Clinical Medicine,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
睾酮血管紧张素Ⅱ心肌成纤维细胞增殖胶原ERK1/2乳鼠
Keywords:
testosteroneangiotensinⅡcardiac fibroblastproliferationcollagenERK1/2neonatal rat
分类号:
R542.2
摘要:
目的:观察睾酮对血管紧张素Ⅱ(AngⅡ)诱导的乳鼠心肌成纤维细胞(CF)增殖和胶原合成的影响。方法:分离、培养CF,分别用无血清DMEM培养液(对照组)、含10-6 mol/L AngⅡ的无血清DMEM培养液(AngⅡ组)、含30 nmol/L睾酮的无血清DMEM培养液(睾酮组)、含30 nmol/L睾酮和10-6 mol/L AngⅡ的无血清DMEM培养液(睾酮+AngⅡ组)培养24 h,应用流式细胞技术检测细胞周期分布,VG染色法检测胶原含量,免疫细胞化学染色法检测磷酸化ERK1/2(pERK1/2)的表达。结果:AngⅡ组S期细胞比例、胶原含量和pERK1/2表达水平较对照组和睾酮组明显增加,睾酮+AngⅡ组S期细胞比例、胶原含量和pERK1/2表达水平较AngⅡ组明显降低(S期细胞比例:FAngⅡ=30.403,F睾酮=5.812,F交互=18.073,P<0.05;胶原含量:FAngⅡ=10.365,F睾酮=4.533,F交互=42.049,P<0.05;pERK1/2表达水平:FAngⅡ=174.762,F睾酮=184.828,F交互=152.240,P<0.05)。结论:睾酮可能通过抑制ERK1/2的活化,抑制AngⅡ诱导的CF的增殖和胶原合成。
Abstract:
Aim: To explore the effect and the mechanism of testosterone on proliferation and collagen synthesis of neonatal rat cardiac fibroblast(CF) induced by angiotensinⅡ(AngⅡ). Methods: CFs derived from the neonatal rat were cultured with serumfree DMEM culture(control group), 10-6 mol/L Ang Ⅱ (Ang Ⅱ group), 30 nmol/L testosterone(testosterone group), or 30 nmol/L testosterone and 10-6 mol/L Ang Ⅱ(testosterone+Ang Ⅱ group) for 24 h, respectively. Flow cytometry technique was used to detect the cell cycle distribution.VG staining method was used to detect the contents of collagen, and immunocytochemistry method was used to detect phosphorylated ERK1/2(pERK1/2) expression. Results: S phase cells proportion, collagen content and pERK1/2 expression level of Ang Ⅱ group were significantly higher than those of the control group and testosterone group, and these indexes of testosterone+Ang Ⅱ group were lower than those of Ang Ⅱ group(S phase cells proportion:FAng Ⅱ=30.403,Ftestosterone=5.812,Finteraction=18.073,P<0.05;collagen content:FAngⅡ=10.365,Ftestosterone=4.533,Finteraction=42.049,P<0.05;pERK1/2 expression level:FAng Ⅱ=174.762,Ftestosterone=184.828,Finteraction=152.240,P<0.05).Conclusion: Testosterone can inhibit CF proliferation and collagen synthesis induced by Ang Ⅱ, and the mechanism may be related to the inhibition of ERK1/2 activation.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省教育厅科学技术研究重点项目(基础研究计划)13A320436;河南省医学科技攻关计划项目2011020038;河南省卫生科技创新型人才工程中青年科技人才项目4099;河南省科技厅河南省基础与前沿科技研究计划项目;郑州大学第一附属医院青年创新基金;#通讯作者,女,1974年6月生,博士,副主任医师,研究方向:心肌重构的机制及防治方法,Email:2414714561@qq.com
更新日期/Last Update: 1900-01-01