[1]武俊芳),牛杰),李晓鹏),等.血管紧张素Ⅱ对小鼠骨髓间充质干细胞中ERK1/2和NFκB信号途径介导的炎症反应的影响*[J].郑州大学学报(医学版),2014,(04):465.
 WU Junfang,NIU Jie,LI Xiaopeng,et al.Effect of angiotensin Ⅱ on inflammatory responses of mouse bone marrow mesenchymal stem cell mediated by ERK1/2 and NFκB pathways[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(04):465.
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血管紧张素Ⅱ对小鼠骨髓间充质干细胞中ERK1/2和NFκB信号途径介导的炎症反应的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年04期
页码:
465
栏目:
论著
出版日期:
2014-07-20

文章信息/Info

Title:
Effect of angiotensin Ⅱ on inflammatory responses of mouse bone marrow mesenchymal stem cell mediated by ERK1/2 and NFκB pathways
作者:
武俊芳1)牛杰1)李晓鹏2)张芬熙34)#范文艳1)李颖虹1)郭志刚1)
1)新乡医学院形态学实验室 新乡 4530032)新乡医学院第三附属医院眼科 新乡 4530033)新乡医学院三全学院解剖学教研室 新乡 4530034) 新乡医学院干细胞与生物治疗研究中心 新乡 453003
Author(s):
WU Junfang1) NIU Jie1)LI Xiaopeng2)ZHANG Fenxi34)FAN Wenyan1)LI Yinghong1)GUO Zhigang1)
1)Laboratory of Morphology, Xinxiang Medical University, Xinxiang 4530032)Department of Ophthalmology, the Third Affiliated Hospital, Xinxiang Medical University, Xinxiang 4530033)Department of Anatomy, Sanquan College, Xinxiang Medical University, Xinxiang 4530034)Stem Cell and Biotheraphy Technology Research Center,Xinxiang Medical University, Xinxiang 453003
关键词:
血管紧张素Ⅱ骨髓间充质干细胞ERK1/2NFκB炎症反应小鼠
Keywords:
angiotensin Ⅱbone marrow mesenchymal stem cellERK1/2NFκBinflammatory responsemouse
分类号:
R331
摘要:
目的:研究血管紧张素Ⅱ(Ang Ⅱ)对小鼠骨髓间充质干细胞(bmMSC)炎症反应的影响。方法:分别采用0、10-8、10-7、10-6和10-5 mol/L Ang Ⅱ体外诱导培养bmMSC 12 h,然后应用ELISA法检测细胞TNFα和IL6的分泌水平,RTPCR法检测细胞TNFα和IL6 mRNA的表达水平, Western blot法检测细胞中ERK1/2和NFκBP65蛋白磷酸化程度。结果:5组bmMSC TNFα和IL6分泌水平、mRNA表达水平,ERK1、ERK2和NFκBP65蛋白磷酸化程度差异均有统计学意义(F=34.562、53.940、24.622、30.208、96.129、106.293和24.752,P<0.001)。与0 mol/L Ang Ⅱ组比较,10-8、10-7、10-6和10-5 mol/L Ang Ⅱ组细胞TNFα和IL6分泌水平升高(P<0.05),TNFα和IL6 mRNA表达水平升高(P<0.05),ERK1/2和NFκBP65磷酸化程度升高(P<0.05),并且表现出一定的剂量依赖性(P<0.05)。结论:Ang Ⅱ可通过活化ERK1/2和NFκB信号途径介导bmMSC的炎症反应。
Abstract:
Aim: To study the effect of angiotensin Ⅱ(Ang Ⅱ) on inflammatory responses of mouse bone marrow mesenchymal stem cell(bmMSC). Methods: bmMSCs were treated with 0,10-8,10-7,10-6 and 10-5 mol/L Ang Ⅱ in vitro for 12 h. The secretion levels of TNFα and IL6 of these cells were measured using ELISA method. The expressions of TNFα and IL6 mRNA were measured by RTPCR. The phosphorylated degree of ERK1/2 and NFκBP65 protein were measured by Western blot. Results: The differences in TNFα and IL6 secretion levels, TNFα and IL6 mRNA expressions, and the phosphorylated degree of ERK1/2 and NFκBP65 protein were significant among the five groups(F=34.562,53.940,24.622,30.208,96.129,106.293 and 24.752,P<0.001).Compared with those of the 0 mol/L Ang Ⅱ group, the secretion levels and mRNA expression of TNFα and IL6, and the phosphorylated degree of ERK1/2, NFκBP65 protein increased with the concentration of Ang Ⅱ increasing(P<0.05). Conclusion: Ang Ⅱ could mediate inflammatory responses in bmMSCs via activation of ERK1/2 and NFκB pathways.

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备注/Memo

备注/Memo:
*河南省教育厅自然科学基金资助项目2010A310006;#通讯作者,女,1978年8月生,硕士,讲师,研究方向:干细胞,Email:fxzhang0824@gmail.com
更新日期/Last Update: 1900-01-01