[1]王富霞,夏熙郑#,刘待见.慢性阻塞性肺疾病模型大鼠骨骼肌组织中Caspase12和mCalpain的表达[J].郑州大学学报(医学版),2014,(04):508.
 WANG Fuxia,XIA Xizheng,LIU Daijian.Expression of Caspase12 and mCalpain in rat skeletal muscle in the COPD model[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(04):508.
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慢性阻塞性肺疾病模型大鼠骨骼肌组织中Caspase12和mCalpain的表达
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年04期
页码:
508
栏目:
论著
出版日期:
2014-07-20

文章信息/Info

Title:
Expression of Caspase12 and mCalpain in rat skeletal muscle in the COPD model
作者:
王富霞夏熙郑#刘待见
郑州大学第二附属医院呼吸内科 郑州 450014
Author(s):
WANG FuxiaXIA XizhengLIU Daijian
Department of Respiratory Disease,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014
关键词:
慢性阻塞性肺疾病骨骼肌萎缩Caspase12mCalpain细胞凋亡大鼠
Keywords:
chronic obstructive pulmonary diseaseskeletal muscle atrophyCaspase12mCalpaincell apoptosisrat
分类号:
R562
摘要:
目的:探讨慢性阻塞性肺疾病(COPD)模型大鼠骨骼肌组织中Caspase12和mCalpain的表达情况。方法:将40只健康雄性Wistar大鼠随机分为COPD模型组和对照组各20只,模型组采用反复熏香烟加气道内滴猪胰弹性蛋白酶法建立COPD模型。采用TUNEL法测定2组大鼠骨骼肌(膈肌、趾长伸肌)细胞凋亡率,采用免疫组化法、RTPCR检测2组大鼠骨骼肌内Caspase12和mCalpain蛋白及mRNA的表达。结果:与对照组相比,模型组大鼠膈肌、趾长伸肌的凋亡率增加(t=23.190和28.184,P<0.001),Caspase12和 mCalpain 蛋白和mRNA的表达亦增强(P<0.001)。模型组大鼠膈肌、趾长伸肌中Caspase12和mCalpain 蛋白与mRNA的表达有关(r=0.885和0.787,P<0.05;r=0.862和0.774,P<0.05)。结论:Caspase12可能参与 COPD 大鼠骨骼肌萎缩,mCalpain可能通过激活 Caspase12 参与该过程。
Abstract:
Aim: To study the expression and significance of Caspase12 and mCalpain in COPD rats skeletal muscle atrophy.Methods:A total of 40 healthy male Wistar rats were randomly divided into model group(n=20) and control group(n=20). COPD model rats were copied by tabocco smoke inhalation and intracheally given PEE successfully.Skeletal muscle apoptosis rate was evaluated by TUNEL method.The expression of Caspase12 and mCalpain mRNA and protein in rat skeletal muscle were detected by reverse transcriptionpolymerase chain reaction (RTPCR) and immunohistochemical respectively.Results: Compared with control group,the rates of muscle apoptosis in both diaphragmatic muscle and long extensor muscle digits of the model group were increased(t=23.190,28.184;P<0.001),and the expression of Caspase12 and mCalpain protein and mRNA were significantly enhanced (P<0.001).Caspase12 and mCalpain had significant correlation(r=0.885,0.787,P<0.05;r=0.862,0.774,P<0.05).Conclusion: Reticulum apoptosis pathway, which involving Caspase12 and mCalpain, may be responsible for COPD rats skeletal muscle atrophy.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,男,1955年12月生,本科,教授,研究方向:慢性阻塞性肺疾病和呼吸系统感染性疾病,Email:xiaxizheng@126.com
更新日期/Last Update: 1900-01-01