[1]宋群立),王蕾),郝永伟),等.高效毛细管电泳法测定盐酸马尼地平片中盐酸马尼地平的含量[J].郑州大学学报(医学版),2014,(04):546.
 SONG Qunli),WANG Lei),HAO Yongwei),et al.Determination of manidipine hydrochloride in manidipine hydrochloride tablets by high performance capillary electrophoresis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(04):546.
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高效毛细管电泳法测定盐酸马尼地平片中盐酸马尼地平的含量
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年04期
页码:
546
栏目:
应用研究
出版日期:
2014-07-20

文章信息/Info

Title:
Determination of manidipine hydrochloride in manidipine hydrochloride tablets by high performance capillary electrophoresis
作者:
宋群立1)王蕾2)郝永伟2)张超峰2)史进进2)张红岭2)#
1)许昌幼儿师范学校 许昌 4617002)郑州大学药学院 郑州 450001
Author(s):
SONG Qunli1) WANG Lei2) HAO Yongwei2) ZHANG Chaofeng2) SHI Jinjin2) ZHANG Hongling2)
1)Xuchang Kindergarten Training School, Xuchang 4617002)School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001
关键词:
盐酸马尼地平片毛细管电泳
Keywords:
manidipine hydrochloride tabletcapillary electrophoresis
分类号:
R917
摘要:
目的:建立以高效毛细管电泳法测定盐酸马尼地平片中盐酸马尼地平含量的方法。方法:以熔融石英毛细管柱(45 cm×75 μm,有效长度37 cm)为固定相,运行缓冲液为30 mmol/L醋酸盐缓冲液(pH 4.5),压力进样(3.4 kPa×5 s),工作电压25 kV,柱温25 ℃,检测波长228 nm。结果:盐酸马尼地平浓度在0.025~0.250 g/L的范围内线性关系良好(r=0.999 4),高、中、低浓度平均回收率分别为99.08%、98.19%和97.14%,RSD分别为0.31%、0.60%和1.51%。结论:该方法简便、快速、经济、重复性好,可用于盐酸马尼地平片中盐酸马尼地平含量的测定。
Abstract:
Aim: To establish a method for the determination of manidipine hydrochloride in manidipine hydrochloride tablets by high performance capillary electrophoresis. Methods: The determination was performed under the following conditions: fusedsilica capillary of 45 cm×75 μm(37 cm of effective length);running buffer: 30 mmol/L acetate buffer(pH 4.5);sample injection method(3.4 kPa×5 s);running voltage: 25 kV;column temperature: 25 ℃;detection wavelength: 228 nm. Results: The linear range of manidipine hydrochloride was from 0.025 to 0.250 g/L(r=0.999 4). The average recoveries and RSD of manidipine hydrochloride at low, middle and high concentrations were 97.14% with RSD of 1.51%,98.19% with RSD of 0.60%, and 99.08% with RSD of 0.31%,respectively. Conclusion: The method is simple, rapid,economic and reproducible. It can be used to detect the content of manidipine hydrochloride in manidipine hydrochloride tablets.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,女,1973年7月生,硕士,副教授,研究方向:肿瘤靶向制剂及体内药物分析,Email:zhanghongling729@126.com
更新日期/Last Update: 1900-01-01