[1]曹振杰),杜俊鹏),杨婵婵),等.先天性巨结肠肠壁组织中L1CAM的表达[J].郑州大学学报(医学版),2014,(04):555.
 CAO Zhenjie),DU Junpeng),YANG Chanchan),et al.Expression of L1CAM in intestinal wall tissues of Hirschsprung disease[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(04):555.
点击复制

先天性巨结肠肠壁组织中L1CAM的表达
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年04期
页码:
555
栏目:
应用研究
出版日期:
2014-07-20

文章信息/Info

Title:
Expression of L1CAM in intestinal wall tissues of Hirschsprung disease
作者:
曹振杰1)杜俊鹏1)杨婵婵2)孙雪花1)黄华1)谷雅川1)陈琦1)#
1)郑州大学第三附属医院小儿外科 郑州 4500522)郑州市中心医院外科 郑州 450007
Author(s):
CAO Zhenjie1)DU Junpeng1)YANG Chanchan2)SUN Xuehua1)HUANG Hua1)GU Yachuan1)CHEN Qi1)
1)Department of Pediatrics, the Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Department of Surgery, the Central Hospital of Zhengzhou, Zhengzhou 450007
关键词:
先天性巨结肠L1CAM
Keywords:
Hirschsprung disease L1CAM
分类号:
R726.5
摘要:
目的:探讨先天性巨结肠(HD)肠壁组织中L1CAM的表达情况,从而分析L1CAM与HD的关系。方法:利用免疫组化SP法和RTPCR法检测48例HD患儿狭窄段和扩张段肠壁组织以及46例正常结肠肠壁组织中L1CAM蛋白和mRNA的表达。结果:HD狭窄段肠壁组织中L1CAM蛋白表达量为(13.147 0±0.519 8),低于HD扩张段及正常结肠肠壁组织的(16.961 8±0.905 4)、(17.908 3±0.740 6)(t配对/t=27.697、25.346,P均<0.001)。HD狭窄段肠壁组织中L1CAM mRNA表达量为(4.316 4±0.387 0),低于HD扩张段及正常结肠肠壁组织的(5.717 8±0.486 0)、(6.344 3±0.236 6)(t配对/t=19.661、14.237,P均<0.001)。狭窄段肠壁组织中L1CAM蛋白和mRNA的表达呈正相关(r=0.968,P<0.001)。结论:L1CAM蛋白和mRNA的异常低表达可能参与了HD的发生过程。
Abstract:
Aim: To investigate the expression of L1CAM in intestinal wall tissues of Hirschsprung disease(HD), and to analyze the relationship between L1CAM and HD. Methods: SP method and RTPCR were used to detected the protein and mRNA expression of L1CAM in the stenotic and ectatic intestinal wall tissues of 48 patients with HD and 46 cases of normal colon intestinal wall tissues. Results: The protein expression of L1CAM in the stenotic intestinal wall tissues of HD was (13.147 0±0.519 8),which was lower than those of the ectatic intestinal wall tissues of HD (16.961 8±0.905 4) and the normal colon intestinal wall tissues (17.908 3±0.740 6) (tpaired/t=27.697, 25.346, P<0.001). The mRNA expression of L1CAM in the stenotic intestinal wall tissues of HD was (4.316 4±0.387 0),which was lower than those of the ectatic intestinal wall tissues of HD (5.717 8±0.486 0) and the normal colon intestinal wall tissues (6.344 3±0.236 6) (tpaired/t=19.661, 14.237, P<0.001). The protein and mRNA expression of L1CAM in the stenotic intestinal wall tissues of HD had the positive relationship(r=0.968, P<0.001). Conclusion: Abnormal low expression of L1CAM mRNA and protein in the stenotic intestinal wall tissues may be involved in the pathogenesis of HD.

参考文献/References:

[1]陈卫坚,刘筱娴.先天性巨结肠发病影响因素研究进展[J].中国社会医学杂志,2008,25(5):311 [2]Moore SW.The contribution of associated congenital anomalies in understanding Hirschsprung's disease[J].Pediatr Surg Int,2006,22(4):305 [3]Moore SW,Zaahl MG.Tissue specific somatic mutations and aganglionosis in Hirschsprung's disease[J].J Pediatr Surg,2014,49(2):258 [4]Heiz M,Grünberg J,Schubiger PA,et al.Hepatocyte growth factorinduced ectodomain shedding of cell adhesion molecule L1: role of the L1 cytoplasmic domain[J].J Biol Chem,2004,279(30):31149 [5]Fernández RM,NúezTorres R,GarcíaDíaz L,et al.Association of Xlinked hydrocephalus and Hirschsprung disease: report of a new patient with a mutation in the L1CAM gene[J].Am J Med Genet A,2012,158A(4):816 [6]陈琦,王志,曹振杰,等.先天性巨结肠患儿结肠肌间神经丛内神经节细胞中NKX21的表达[J].郑州大学学报:医学版,2012,47(3):365 [7]杨合英,刘秋亮,王家祥,等.多发性节段型肠无神经节细胞症的临床分析[J].中华医学杂志,2005,85(39):2772 [8]Boman F,Corsois L,Paraf F.Hirschsprung's disease: practical considerations[J].Ann Pathol,2004,24(6):486 [9]彭梓月,韩毅敏,王晶.L1CAM在肿瘤组织中的研究进展[J].现代肿瘤医学,2013,21(3):665 [10]Anderson RB,Turner KN,Nikonenko AG,et al.The cell adhesion molecule 11 is required for chain migration of neural crest cells in the developing mouse gut[J].Gastroenterology,2006,130(4):1221

备注/Memo

备注/Memo:
#通讯作者,男,1963年4月生,硕士,主任医师,研究方向:小儿消化道畸形,Email:chen63qi@126.com
更新日期/Last Update: 1900-01-01