[1]张倩),张宏伟),夏建华),等.线粒体分裂蛋白抑制剂对匹鲁卡品致痫大鼠海马神经元的保护作用*[J].郑州大学学报(医学版),2014,(06):773.
 ZHANG Qian),ZHANG Hongwei),XIA Jianhua),et al.Effect of mitochondrial division inhibitor on hippocampal neuro in pilocarpineinduced seizures of rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(06):773.
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线粒体分裂蛋白抑制剂对匹鲁卡品致痫大鼠海马神经元的保护作用*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年06期
页码:
773
栏目:
论著
出版日期:
2014-11-20

文章信息/Info

Title:
Effect of mitochondrial division inhibitor on hippocampal neuro in pilocarpineinduced seizures of rats
作者:
张倩12)张宏伟13)夏建华14)连亚军1)#谢南昌1)#
1)郑州大学第一附属医院神经内科 郑州 4500522)河南省人民医院康馨综合病房 郑州 4500033)中国平煤神马医疗集团总医院神经内科 平顶山 4671004)驻马店市中心医院神经内科 驻马店 463000
Author(s):
ZHANG Qian12) ZHANG Hongwei13) XIA Jianhua14) LIAN Yajun1) XIE Nanchang1)
1)Department of Neurology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 4500522)Department of Cardiovasology(Elderly), Henan Provincial People′s Hospital, Zhengzhou 4500033)Department of Neurology, Medical Group General Hospital of Pingdingshan Coal and Shenma Company Ltd. in China, Pingdingshan 4671004)Department of Neurology, the Central Hospital of Zhumadian, Zhumadian 463000
关键词:
癫痫线粒体分裂Mdivi1氧化应激凋亡
Keywords:
epilepsy mitochondrial fission Mdivi1 oxidative stress apoptosis
分类号:
R742.1
摘要:
目的:研究线粒体分裂蛋白抑制剂(Mdivi1)对匹鲁卡品(PILO)致痫大鼠海马神经元的保护作用及其机制。方法:121只雄性SD大鼠随机分成对照(CON)组、PILO组、PILO+DMSO组和PILO+Mdivi1组,PILO组又分为癫痫持续状态(SE)2 h、8 h、24 h和72 h 4个亚组。观察各组大鼠行为学改变,并分别采用Nissl染色法检测神经元损伤,比色法检测SOD活力及MDA含量,Western blot和RTPCR法检测Drp1、细胞色素C(Cyt C)、凋亡诱导因子(AIF)和cleaved半胱天冬酶3(caspase3)表达水平。结果:与PILO组(114.571±5.420)相比,PILO+Mdivi1组海马神经元数目增加[(157.429±6.183);F=68.693,P<0.001]。与PILO组SE 24 h亚组[(113.429±4.980),(6.102±0.193) ]相比,PILO+Mdivi1组SOD活力增加[(138.571±3.380);F=21.779,P<0.001],MDA含量减少[(4.353±0.231);F=27.226,P<0.001]。与PILO组[(0.594±0.020),(1.099±0.015) ]相比,PILO+Mdivi1组线粒体Cyt C增加[(0.897±0.015);F=296.177,P<0.001],细胞质Cyt C减少[(0.433±0.010);F=562.684,P<0.001]。与PILO组[(0.878±0.037),(1.465±0.076)] 相比,PILO+Mdivi1组线粒体AIF增加[(1.279±0.051);F=59.298,P<0.001],细胞核AIF减少[(0.896±0.044);F=46.424,P<0.001]。与PILO组(0.587±0.192) 相比,PILO+Mdivi1组cleaved caspase3减少[(0.463±0.022);F=40.500,P<0.001]。结论:Mdivi1对癫痫大鼠海马神经元有保护作用,其机制可能与降低氧化应激,抑制Cyt C释放、AIF移位及caspase3激活有关。
Abstract:
Aim: To investigate the effect of mitochondrial division inhibitor (Mdivi1) in pilocarpineinduced seizures and its mechanism. Methods: A total of 121 male SD rats were randomly divided into control, PILO, PILO+Mdivi1 and PILO+DMSO group, the PILO group was further divided into four subgroups according to observation time. The behavior changes of each group were observed, while the pathological damage and levels of superoxide dismutase and malondialdehyde were detected by Nissl staining, xanthine oxidase and thiobarbituric acid method. The expression of dynaminrelated protein 1, Cyt C, AIF and cleavedcaspase3 were measured by Western blot analysis and RTPCR. Results: Compared with PILO group (114.571±5.420), the surviving neuron number of PILO+Mdivi1 group was increased[(157.429±6.183);F=68.693,P<0.001]. Compared with PILO SE 24 h subgroup [(113.429±4.980),(6.102±0.193)],the activity of SOD[(138.571±3.380);F=21.779,P<0.001]was increased and the content of MDA(4.353±0.231);F=27.226,P<0.001]was decreased in PILO+Mdivi1 group. Compared with PILO group[(0.594±0.020),(1.099±0.015)], the expression of mitoCyt C[(0.897±0.015);F=296.177,P<0.001]was increased and the expression of cytoCyt C[(0.433±0.010);F=562.684,P<0.001]was decreased in PILO+Mdivi1 group. Compared with PILO group[(0.878±0.037),(1.465±0.076)], the expression of mitoAIF[(1.279±0.051);F=59.298,P<0.001]was increased and the expression of nuAIF[(0.896±0.044);F=46.424,P<0.001]was decreased in PILO+Mdivi1 group. Compared with PILO group (0.587±0.192), the expression of cleaved caspase3[(0.463±0.022);F=40.500,P<0.001]was decreased of PILO+Mdivi1 group. Conclusion: Mdivi1 has neuroprotective effect on hippocampal neuro in pilocarpineinduced seizures, and the underlying mechanism may be due to the suppression of oxidative stress and inhibition of the release of Cyt C, translocation of AIF and activation of caspase3.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目81201012;81371438;#通讯作者,连亚军,女,1964年4月生,博士,主任医师,教授,研究方向:癫痫、头痛、脑血管病、肌张力障碍的基础与临床,Email:yajunlian@163.com;谢南昌,男,1984年1月生,博士,主治医师,研究方向:癫痫的基础与临床, Email:hnxienanchang@163.com
更新日期/Last Update: 2014-11-26