[1]朱海鹏△.萝卜硫素对LNCaP细胞增殖、周期、凋亡及IGFBP3、NFκB表达的影响[J].郑州大学学报(医学版),2014,(06):801.
 ZHU Haipeng.Effects of sulforaphane on proliferation, cell cycle and apoptosis of LNCaP and expressions of IGFBP3 and NFκB[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(06):801.
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萝卜硫素对LNCaP细胞增殖、周期、凋亡及IGFBP3、NFκB表达的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年06期
页码:
801
栏目:
论著
出版日期:
2014-11-20

文章信息/Info

Title:
Effects of sulforaphane on proliferation, cell cycle and apoptosis of LNCaP and expressions of IGFBP3 and NFκB
作者:
朱海鹏△
郑州大学第五附属医院泌尿外科 郑州 450052
Author(s):
ZHU Haipeng
Department of Urology, the Fifth Affiliated Hospital, Zhengzhou University,Zhengzhou 450052
关键词:
萝卜硫素前列腺癌IGFBP3NFκBLNCaP细胞
Keywords:
sulforaphane prostate cancer IGFBP3 NFκB LNCaP cell
分类号:
R737.25
摘要:
目的:观察萝卜硫素(SFN)对人前列腺癌LNCaP细胞增殖、周期及凋亡的影响以及IGFBP3及NFκB在此过程中的变化。方法:观察不同浓度SFN对LNCaP细胞增殖的影响后将LNCaP细胞分为4组:对照组、SFN处理组、IGFBP3 siRNA+SFN处理组以及IGFBP3 siRNA处理组。MTT法分析各组细胞的增殖情况;流式细胞技术分析各组细胞的细胞周期及凋亡。实时荧光定量PCR技术和Western blot技术分析各组细胞IGFBP3、NFκB mRNA和蛋白表达的变化。结果:随SFN浓度的升高,LNCaP细胞存活率呈下降趋势(F=1 391.781, P<0.001)。IGFBP3抑制能有效减弱SFN诱导的细胞增殖抑制、G2期抑制与凋亡(F交互=271.130、121.133和95.000, P<0.05)。IGFBP3抑制可降低SFN处理后IGFBP3 mRNA和蛋白表达的升高,升高SFN处理后NFκB mRNA和蛋白表达的降低(F交互=8.595和279.490,P<0.05)。结论:SFN对LNCaP细胞的抑制作用可能与IGFBP3有关,后者可能通过改变NFκB的表达来发挥作用。
Abstract:
Aim: To investigate the effects of sulforaphane on proliferation, cell cycle and apoptosis of LNCaP and expressions of IGFBP3 and NFκB. Methods: The effects of SFN at different concentrations on LNCaP cells were observed. LNCaP cells were divided into four groups, including control group, 20 μmol/L SFN treated group, IGFBP3 siRNA+20 μmol/L SFN treated group and IGFBP3 siRNA treated group. MTT assay was used to evaluate the cell proliferation.Cell cycle arrest and the cell apoptosis were detected by flow cytometry.Further more, the expressions of IGFBP3 and NFκB in mRNA level and protein level were analyzed by realtime quantitative PCR and Western blot respectively.Results: SFN could inhibit the proliferation of LNCaP cells in a dosedependent manner(F=1 391.781,P<0.001). The inhibition of IGFBP3 could antagonize the proliferation inhibition, G2 phase arrest and apoptosis induced by SFN(Finteraction=271.130,121.133 and 95.000, P<0.05).The inhibition of LNCap could depress the increased expression of IGFBP3 mRNA and protein after SFN treatment,while increase the reduced expressions of NFκB mRNA and protein by SFN treatment(Finteraction=8.595,279.490,P<0.05).Conclusion: SNF can inhibit the LNCaP cells′ proliferation and the mechanism may associate with the changes of IGFBP3, which may play a role in this process by changing the level of NFκB.

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备注/Memo

备注/Memo:
△男,1963年11月生,本科,副主任医师,研究方向:前列腺癌的防治,Email:zhuhaipeng081611@163.com
更新日期/Last Update: 2014-11-26