[1]刘洋),李攀阳),张伟华),等.离体猪心脏的保存及功能评价[J].郑州大学学报(医学版),2014,(06):845.
 LIU Yang),LI Panyang),ZHANG Weihua),et al.Preservation and cardiac function evaluation of isolated heart from pig in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(06):845.
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离体猪心脏的保存及功能评价
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年06期
页码:
845
栏目:
应用研究
出版日期:
2014-11-20

文章信息/Info

Title:
Preservation and cardiac function evaluation of isolated heart from pig in vitro
作者:
刘洋1)李攀阳2)张伟华1)秦广启3)陈雪梅2)廖秋明3)Steen Stig3)乔晨晖1)#
1)郑州大学第一附属医院心血管外科 郑州 4500522)郑州大学基础医学院人体解剖学教研室 郑州 4500013)瑞典隆德大学生命科学实验室 隆德 22594
Author(s):
LIU Yang1)LI Panyang2)ZHANG Weihua1)QIN Guangqi3)CHEN Xuemei2)LIAO Qiuming3)STEEN Stig3)QIAO Chenhui1)
1)Department of Cardiovascular Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 4500522)Department of Human Anatomy,College of Basic Medical Sciences,Zhengzhou University, Zhengzhou 4500013)Laboratory of Life and Science, Lund University, Lund 22594
关键词:
心脏功能心脏保存离体心脏
Keywords:
heart functionheart preservationisolated heartpig
分类号:
R654.2
摘要:
目的:探索一种新型供体心脏体外保存及功能评价的方法。方法:将血型一致的12头瑞典健康家猪作为实验对象,随机分为心脏供体组和血液供体组两组,每组6头。用体外循环机以不同流量(0.76、1.00、2.00、3.00、4.00 L/min)在37 ℃条件下灌注离体心脏,监测动静脉血气和离体心脏血压、心率、心肌耗氧量及氧摄取率。结果:不同灌注流量下,离体心脏心肌耗氧量、心率、收缩压、平均压、左室收缩压差异均有统计学意义(F=23.500,331.010,77.701,10.101和89.301,P均<0.001)。灌注流量较高,离体心脏上述各指标也较高(F=0.460,P>0.05)。不同灌注流量组心肌氧摄取率差异无统计学意义(F=0.426,P=0.788)。结论:该模型可使离体心脏心功能保持良好并得到较好的保存。
Abstract:
Aim: To explore a new pig isolated heart preservation and heart function evaluation method. Methods: A total of 12 Swedish health home pigs were divided into two groups according to blood type, the blood donor group and isolated heart donor group, and 6 in each group. The cardiopulmonary bypass machine were used to perfuse isolated hearts at different perfusion flow(0.76,1.00,2.00,3.00, and 4.00 L/min) under normal temperature (37 ℃). The myocardial oxygen consumption, heart rate, blood pressure and hemodynamic index of isolated hearts were monitored. Results: There were significant differences in myocardial oxygen consumption, heart rate, systolic blood pressure, average pressure, left ventricular systolic blood pressure of isolated hearts with different perfusion flow(F=23.500,331.010,77.701,10.101 and 89.301,P<0.001).The perfusion flow was higher, the indexes mentioned above were higher in isolated heart(P<0.05).There was no statistically significant difference in myocardial oxygen uptake rate among the 5 groups(F=0.426,P=0.788).Conclusion: The method for pig isolated heart preservation and accurate evaluation of cardiac function is established.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,男,1961年9月生,博士,教授,研究方向:心脏移植,Email:qchenhui@hotmail.com
更新日期/Last Update: 2014-11-26