[1]张军辉)△,严家芹),赵玉林).雷帕霉素对缺氧条件下Hep2细胞增殖及VEGF、HIF1α表达的影响[J].郑州大学学报(医学版),2014,(06):859.
 ZHANG Junhui,YAN Jiaqin,ZHAO Yulin.Effects of rapamycin on proliferation of hypoxiainduced laryngeal cancer Hep2 cells and expressions of VEGF and HIF1α[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(06):859.
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雷帕霉素对缺氧条件下Hep2细胞增殖及VEGF、HIF1α表达的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2014年06期
页码:
859
栏目:
应用研究
出版日期:
2014-11-20

文章信息/Info

Title:
Effects of rapamycin on proliferation of hypoxiainduced laryngeal cancer Hep2 cells and expressions of VEGF and HIF1α
作者:
张军辉1)△严家芹2)赵玉林3)
1)郑州大学第三附属医院耳鼻咽喉科 郑州 4500522)郑州大学第一附属医院肿瘤科 郑州 4500523)郑州大学第一附属医院耳鼻咽喉科 郑州 450052
Author(s):
ZHANG Junhui1)YAN Jiaqin2) ZHAO Yulin3)
1)Department of Otorhinolaryngology,the Third Affiliated Hospital,Zhengzhou University, Zhengzhou 4500522)Department of Oncology,the First Affiliated Hospital,Zhengzhou University, Zhengzhou 4500523)Department of Otorhinolaryngology,the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052
关键词:
喉癌缺氧雷帕霉素Hep2HIF1αVEGF
Keywords:
laryngeal carcinoma hypoxia rapamycin Hep2 HIF1α VEGF
分类号:
R739.65
摘要:
目的:探讨雷帕霉素对缺氧条件下人喉癌Hep2细胞增殖及VEGF、HIF1α表达的影响。方法:在缺氧条件下,采用MTT法检测0、5、10、20 nmol/L雷帕霉素处理12、24、36、48、60、72 h后Hep2细胞的增殖活性,ELISA检测0、5、10、20 nmol/L雷帕霉素处理24 h细胞培养上清液VEGF蛋白含量的变化,Western blot检测0、5、10、20 nmol/L雷帕霉素处理16 h细胞HIF1α蛋白表达的变化。结果:缺氧条件下雷帕霉素对Hep2细胞仍具有明显的增殖抑制作用,且呈浓度和时间依赖性(F浓度=253.132,F时间=213.945,P<0.05)。缺氧条件下雷帕霉素可抑制Hep2细胞VEGF的分泌及HIF1α蛋白的表达(F=7.045、16.218,P<0.05)。结论:雷帕霉素可抑制缺氧条件下Hep2细胞的增殖,其机制可能与抑制Hep2细胞VEGF的分泌及HIF1α蛋白的表达有关。
Abstract:
Aim: To study the effect of rapamycin on the proliferation of hypoxia induced laryngeal cancer Hep2 cells and the expressions of VEGF and HIF1α. Methods: MTT was used to observe the proliferation of Hep2 cells treated with 0,5,10,20 nmol/L rapamycin for 12,24,36,48,60,72 h under hypoxia environment. ELISA was used to determine the content of VEGF in cell culture supernatant after 0,5,10,20 nmol/L rapamycin treatment for 24 h. Western blot was used to detect the expression of HIF1α in the cells after 0,5,10,20 nmol/L rapamycin treatment for 16 h. Results: Rapamycin could inhibit the proliferation of Hep2 cells in a dosedependent and timedependent manner under hypoxia(Fconcentration=253.132,Ftime=213.945,P<0.05), significantly decrease the content of VEGF in Hep2 cell culture supernatant and the expression of HIF1α protein(F=7.045,16.218,P<0.05). Conclusion: Rapamycin could inhibit the proliferation of Hep2 cells under hypoxia environment, which may be involved in its inhibiting the secretion of VEGF and decreasing the expression of HIF1α.

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备注/Memo

备注/Memo:
△男,1978年1月生,博士,主治医师,研究方向:耳鼻喉科基础和临床,Email:zhangjunhui777@126.com
更新日期/Last Update: 2014-11-26