[1]任京力),林玲),王雁梅),等.氯通道阻断剂DIDS对过氧化氢诱导PC12细胞凋亡的影响*[J].郑州大学学报(医学版),2015,(01):17.
 REN Jingli),LIN Ling),WANG Yanmei),et al.Effect of chloride channel blocker DIDS on apoptosis of PC12 cells induced by hydrogen peroxide[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(01):17.
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氯通道阻断剂DIDS对过氧化氢诱导PC12细胞凋亡的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年01期
页码:
17
栏目:
论著
出版日期:
2015-01-20

文章信息/Info

Title:
Effect of chloride channel blocker DIDS on apoptosis of PC12 cells induced by hydrogen peroxide
作者:
任京力1) 林玲2)王雁梅1)王福青2)李超彦2)蔡智慧2)常全忠23)#
1)漯河医学高等专科学校药学系药理学教研室 漯河 462002 2)漯河医学高等专科学校基础部生理学教研室 漯河 462002 3)遵义医学院珠海校区生理学教研室 珠海 519041
Author(s):
REN Jingli1) LIN Ling2) WANG Yanmei1) WANG Fuqing2) LI Chaoyan2) CAI Zhihui2) CHANG Quanzhong2)
1)Department of Pharmacology,Pharmacy Faculty of Luohe Medical College,Luohe 462002 2)Department of Physiology,Basic Medicine Faculty of Luohe Medical College,Luohe 462002 3)Department of Physiology,Zhuhai Campus,Zhuhai Medical University,Zhuhai 519041
关键词:
DIDSPC12细胞凋亡Caspase3Bcl2
Keywords:
DIDSPC12 cellapoptosisCaspase3Bcl2
分类号:
R338.2
摘要:
摘要目的:探讨氯通道阻断剂DIDS抗PC12细胞凋亡的作用机制。方法:取处于对数生长期的PC12细胞,随机分为对照组、模型组(用400 nmol/L H2O2诱导细胞凋亡)和DIDS组(H2O2+DIDS),培养12 h后,用MTT法测定各组细胞的存活率,Western blot法观察凋亡相关蛋白Caspase3和Bcl2蛋白的表达情况,用流式细胞仪检测细胞凋亡率。 结果:400 nmol/L H2O2可以明显诱导PC12细胞损伤,细胞存活率为(35.07±1.23)%;DIDS组细胞存活率明显高于模型组,且具有一定浓度依赖性(F=640.420,P<0.001)。与对照组比较,模型组细胞凋亡率和Caspase3蛋白表达增加,Bcl2蛋白表达降低(P<0.05);与模型组相比,DIDS组Caspase3表达降低,Bcl2蛋白表达增加,细胞凋亡率降低(P<0.05)。 结论:DIDS可能通过抑制Caspase3依赖性凋亡信号通路发挥抗凋亡作用。
Abstract:
AbstractAim: To investigate the mechanism of chloride channel blocker DIDS on apoptosis of PC12 cells. Methods: PC12 cells in logarithmic growth period were randomly divided into control group, model group(induced by 400 nmol/L H2O2 for 12 h) and DIDS group(induced by 400 nmol/L H2O2 and different doses of DIDS for 12 h). The cell survival rate was measured using MTT, the expressions of apoptotic related proteins Caspase3 and Bcl2 were detected by Western blot, and apoptosis was observed by flow cytometry. Results: 400 nmol/L H2O2 could induce PC12 cell damage, and the cell survival rate was (35.07±1.23)%. The survival rate of DIDS group was higher than that of the model group(F=640.420,P<0.001).Compared with those of the control group, the apoptosis rate and the expression of Caspase3 were higher,and Bcl2 expression was lower(P<0.05). Compared with those of the model group, the apoptosis rate and the expression of Caspase3 were lower, and Bcl2 expression was higher(P<0.05). Conclusion: DIDS might be antiapoptotic through inhibiting Caspase3 dependent apoptotic signaling pathway.

参考文献/References:

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目81160157 #通信作者,男,1965年12月生,教授,博士,研究方向:中枢神经系统的损伤及修复,E-mail:cqzchang@sina.com
更新日期/Last Update: 1900-01-01