[1]郭文静),樊红琨),郑春光),等.沉默JP2基因对小鼠心肌细胞SK2通道的影响*[J].郑州大学学报(医学版),2015,(01):55.
 GUO Wenjing),FAN Hongkun),ZHENG Chunguang),et al.Effects of targeting JP2 RNAi on SK2 channel in mouse cardiomyocytes[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(01):55.
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沉默JP2基因对小鼠心肌细胞SK2通道的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年01期
页码:
55
栏目:
论著
出版日期:
2015-01-20

文章信息/Info

Title:
Effects of targeting JP2 RNAi on SK2 channel in mouse cardiomyocytes
作者:
郭文静1)樊红琨1)郑春光2)张桂红1)段萍1)杨阳1)罗天霞1)章茜1)#
1)郑州大学基础医学院生理学教研室 郑州 4500012)河南护理职业学院生理学教研室 安阳 455001
Author(s):
GUO Wenjing1)FAN Hongkun1)ZHENG Chunguang2)ZHANG Guihong1)DUAN Ping1)YANG Yang1)LUO Tianxia1)ZHANG Qian1)
1)Department of Physiology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 2)Department of Physiology,Henan Nursing Vocational College,Anyang 455001
关键词:
junctophilinSK通道RNA干扰心肌细胞小鼠
Keywords:
junctophilinSK channelRNA interferencecardiomyocytemouse
分类号:
R331.3+1
摘要:
摘要目的:探讨心肌细胞JP2与SK2通道的相互作用。方法:构建靶向小鼠JP2基因的siRNA慢病毒载体(LVJP2RNAi1、LVJP2RNAi2),将其转染至小鼠心肌细胞,以转染空载体(LVNCEGFP)和未转染细胞(CtrlNC)作对照。采用实时荧光定量PCR和Western blot法分别检测各组心肌细胞JP2 mRNA和SK2蛋白的表达。结果:转染48 h后,各组小鼠心肌细胞JP2 mRNA表达的差异有统计学意义(F=31.775,P<0.001),LVJP2RNAi1、LVJP2RNAi2组较CtrlNC及LVNCEGFP组细胞降低。小鼠心肌细胞感染LVJP2RNAi2 72 h后,SK2通道蛋白表达被抑制。结论:沉默小鼠心肌细胞JP2基因可抑制SK2通道的功能。
Abstract:
AbstractAim: To investigate the relationship between JP2 and SK2 in the cardiomyocyte.Methods: The recombinants of lentiviral vectors targeting JP2 gene were constructed(LVJP2RNAi1,LVJP2RNAi2) for siRNA. The neonatal mouse cardiac myocytes(NMCMs) were infected with the recombinants. The levels of JP2 mRNA and SK2 protein were detected by quantitative realtime PCR and Western blot. Results: The JP2 mRNA level of the NMCMs among the four groups were different(F=31.775,P<0.001),which were significantly decreased in LVJP2RNAi1,LVJP2RNAi2 groups compared with control and LVNCEGFP groups. Compared with control and LVNCEGFP groups, the expression of the SK2 channel protein in the NMCMs with 72 h infection LVJP2RNAi2 was obviously inhibited. Conclusion: Knockdown of JP2 in the mouse cardiomyocyte could suppress the function of SK2 channel.

参考文献/References:

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目81270248 #通信作者,女,1957年10月生,博士,教授,研究方向:心脏生理学,E-mail:qianzhang@zzu.edu.cn
更新日期/Last Update: 1900-01-01