[1]王丽萍)△,丁一),刘国红),等.五倍子酸对人纤维肉瘤HT1080细胞增殖和凋亡的影响*[J].郑州大学学报(医学版),2015,(01):58.
 WANG Liping,DING Yi,LIU Guohong,et al.Inhibitory effects of gallic acid on human fibrocarcinoma cell HT1080 proliferation and apoptosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(01):58.
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五倍子酸对人纤维肉瘤HT1080细胞增殖和凋亡的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年01期
页码:
58
栏目:
论著
出版日期:
2015-01-20

文章信息/Info

Title:
Inhibitory effects of gallic acid on human fibrocarcinoma cell HT1080 proliferation and apoptosis
作者:
王丽萍1)△丁一1) 刘国红1)石卓2)
1)郑州大学基础医学院组织学与胚胎学教研室 郑州 4500012)吉林大学基础医学院药理学教研室 长春 130021
Author(s):
WANG Liping1) DING Yi1) LIU Guohong1) SHI Zhuo2)
1)Department of Histology and Embryology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 4500012)Department of Pharmacology, Preclinical Medicine College, Jilin University, Changchun 130021
关键词:
五倍子酸HT1080细胞Bcl2Bax
Keywords:
gallic acidHT1080 cellBcl2Bax
分类号:
R961.1
摘要:
摘要目的:研究五倍子酸(GA)对人纤维肉瘤HT1080细胞增殖和凋亡的影响。方法:分别用3.125、6.250、12.500、25.000、50.000和100.000 mg/L的GA处理HT1080细胞48 h后,应用MTT法检测细胞的抑制率;分别以6.250、12.500和25.000 mg/L的GA作用HT1080细胞48 h后,应用流式细胞仪检测细胞凋亡情况,应用免疫细胞化学方法检测GA对HT1080细胞中Bcl2和Bax蛋白表达的影响。结果:GA对HT1080细胞以剂量依赖的方式抑制其增殖(F=71.214,P<0.001)、诱导其凋亡(F=52.217,P<0.001),并能上调Bax蛋白的表达(F=11.024,P<0.001)、下调Bcl2蛋白的表达(F=8.741, P<0.001)。结论:GA可能通过上调Bax蛋白的表达和下调Bcl2蛋白的表达来抑制HT1080细胞增殖和诱导其凋亡。
Abstract:
AbstractAim: To explore the inhibitory effects of gallic acid(GA) on human fibrocarcinoma cell HT1080. Methods: MTT was employed to detect proliferative ability in HT1080 cells treated with 3.125, 6.250, 12.500, 25.000, 50.000 and 100.000 mg/L GA for 48 h, respectively. Flow cytometry was used to examine apoptotic rate in HT1080 cells treated with 6.250, 12.500 and 25.000 mg/L GA for 48 h, respectively. Immunocytochemistry was applied to explore the change of Bcl2 and Bax expression in HT1080 cells.Results: The proliferation inhibition rate of HT1080 cells was enhanced in a dosedependent manner(F=71.214,P<0.001). The number of apoptosis cell increased in a dosedependent manner(F=52.217, P<0.001). GA upregulated Bax expression(F=11.024,P<0.001) and downregulated Bcl2 expression(F=8.741, P<0.001). Conclusion: GA probably inhibit the proliferation and induce apoptosis of HT1080 cells by upregulating Bax and downregulating Bcl2.

参考文献/References:

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相似文献/References:

[1]王丽萍),曾宪旭),牛凤兰),等.五倍子酸对小鼠胃癌MFC和肝癌H22细胞增殖的抑制作用[J].郑州大学学报(医学版),2012,(03):339.
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备注/Memo

备注/Memo:
*河南省科技厅重点攻关课题122102310233 △女,1977年9月生,硕士,讲师,研究方向:肿瘤药理学,E-mail:lp.wang918@126.com
更新日期/Last Update: 1900-01-01