[1]杨德彬),路明)#.川芎嗪对阿霉素诱导的大鼠心肌重塑中TGFβ1表达的影响*[J].郑州大学学报(医学版),2015,(01):71.
 YANG Debin),LU Ming).Effect of ligustrazine on myocardial remodeling in rats with adriamycin induced expression of TGFβ1[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(01):71.
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川芎嗪对阿霉素诱导的大鼠心肌重塑中TGFβ1表达的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年01期
页码:
71
栏目:
应用研究
出版日期:
2015-01-20

文章信息/Info

Title:
Effect of ligustrazine on myocardial remodeling in rats with adriamycin induced expression of TGFβ1
作者:
杨德彬1)路明2)#
1)郑州市儿童医院心血管内科 郑州4500532)徐州医学院附属医院儿科 徐州 221002
Author(s):
YANG Debin1)LU Ming2)
1)Department of Cardiology, Zhengzhou Children′s Hospital, Zhengzhou 4500532)Department of Pediatrics Hospital, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002
关键词:
心肌重塑纤维化川芎嗪TGFβ1阿霉素大鼠
Keywords:
myocardial remodelingfibrosisligustrazineTGFβ1adriamycinrat
分类号:
R541
摘要:
摘要目的:研究川芎嗪对阿霉素(ADM)诱导的大鼠心肌重塑的影响及是否通过改变TGFβ1的表达产生作用。方法:SD大鼠随机分为正常对照组(NC组,n=8;生理盐水2 mL/kg腹腔注射)、模型组(ADM组,n=9;ADM 2 mg/kg腹腔注射)、川芎嗪治疗组(Lig组,n=11;ADM 2 mg/kg+磷酸川芎嗪20 mg/kg腹腔注射)3组。测定各组SD大鼠心重指数(CWI)、左室心脏重量指数(LVWI),光镜下观察心肌病理学变化,免疫组化方法检测各组大鼠心肌组织中Ⅰ、Ⅲ型心肌胶原的表达,Western blot方法检测各组大鼠心肌组织中TGFβ1蛋白的表达。结果:3组大鼠间的心肌纤维化病理改变不同,CWI、LVWI、心肌组织中Ⅰ、Ⅲ型心肌胶原蛋白、Ⅰ/Ⅲ型胶原蛋白比值及TGFβ1蛋白的表达差异有统计学意义(F=5.971、5.556、51.740、57.281、28.934和61.263,P<0.05)。与ADM组相比,Lig组大鼠的心肌纤维化病理改变明显减轻,CWI、LVWI、心肌组织中Ⅰ、Ⅲ型心肌胶原蛋白、Ⅰ/Ⅲ型胶原蛋白比值及TGFβ1蛋白表达明显下降(P<0.05);Lig组大鼠的心肌组织中Ⅰ、Ⅲ型心肌胶原蛋白、Ⅰ/Ⅲ型胶原蛋白比值及TGFβ1蛋白的表达仍高于NC组(P<0.05)。结论:川芎嗪能改善阿霉素诱导的大鼠心肌重塑,其机制可能与影响TGFβ1的表达有关。
Abstract:
AbstractAim: To investigate whether change in TGFβ1 expression is involved in the mechanism by which ligustrazine affects adriamycininduced myocardial remodelling in rats.Methods: SD rats were randomly divided into normal control group(NC group,n=8), model group(ADM group,n=9), and ligustrazine treatment group (Lig group,n=11). For each group, cardiac weight index (CWI) and left ventricular weight index (LVWI) were measured, pathological changes were observed by light microscopy, type Ⅰ, Ⅲ myocardial collagen expression was determined by immunohistochemistry, and TGFβ1 expression in cardiac tissue was detected by Western blot.Results: Myocardial fibrosis, CWI, LVWI, type Ⅰ, Ⅲ myocardial collagen expression, ratio of Ⅰ/Ⅲ myocardial collagens and TGFβ1 protein expression showed significant differences among the three groups (F=5.971, 5.556, 51.740,57.281,28.934,61.263;P<0.05). Compared with ADM group, cardiac fibrosis was significantly alleviated, CWI, LVWI, type Ⅰ, Ⅲ myocardial collagen expression, ratio of type Ⅰ/Ⅲ myocardial collagen and TGFβ1 protein expression significantly decreased in the Lig group (P<0.05); however, type Ⅰ, Ⅲ myocardial collagen expression, ratio of type Ⅰ/Ⅲ myocardial collagen, and TGFβ1 expression in cardiac tissue of the Lig group were still higher than those of NC group(P<0.05). Conclusion: Ligustrazine can improve cardiac remodeling induced by adriamycin, and changes in TGFβ1 may be part of the mechanism.

参考文献/References:

参考文献 [1]冯健,李家富.转化生长因子β/smad信号转导通路与心脏重构[J].医学综述,2007,13(24):1938 [2]钟国强,马国添,刘唐威,等.腹腔注射阿霉素建立扩张型心肌病大鼠模型的方法学改进[J].基础医学与临床,2007,27(11):1289 [3]Zhu J,Teng YH,Wang PE,et al.Effect of ligustrazine on reverse cholesterol transport in foam cells[J].Zhongguo Zhong Yao Za Zhi,2014,39(7):1255 [4]Lan Z,Bi KS,Chen XH.Ligustrazine attenuates elevated levels of indoxyl sulfate, kidney injury molecule1 andclusterin in rats exposed to Cadmium[J].Food Chem Toxicol,2014,63:62 [5]Zheng H,Wang S,Zhou P,et al.Effects of Ligustrazine on DNA damage and apoptosis induced by irradiation[J].Environ Toxicol Pharmacol,2013,36(3):1197 [6]Luo ZL,Tian FZ,Tang LJ,et al.Protective effect of Ligustrazine preconditioning on hepatic energy metabolism in ischemia/reperfusion injury of rat[J].Sichuan Da Xue Xue Bao Yi Xue Ban,2012,43(4):540

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备注/Memo

备注/Memo:
*江苏省科技厅社会发展科技项目BS2004514 #通信作者,男,1960年3月生,硕士,主任医师,副教授,研究方向:小儿心血管疾病,E-mail:xzmclm@163.com
更新日期/Last Update: 1900-01-01