[1]侯长春,梁悦,邬丽红,等.高迁移率族蛋白B1对16HBE细胞血管内皮生长因子表达和分泌的影响*[J].郑州大学学报(医学版),2015,(01):91.
 HOU Changchun,LIANG Yue,WU Lihong,et al.Effects of highmobility group protein B1 on expression and secretion of VEGF in 16HBE cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(01):91.
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高迁移率族蛋白B1对16HBE细胞血管内皮生长因子表达和分泌的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年01期
页码:
91
栏目:
应用研究
出版日期:
2015-01-20

文章信息/Info

Title:
Effects of highmobility group protein B1 on expression and secretion of VEGF in 16HBE cells
作者:
侯长春梁悦邬丽红罗劲刘丽华陈一强#
广西医科大学第一附属医院呼吸疾病研究所 南宁 530021
Author(s):
HOU Changchun LIANG YueWU LihongLUO Jin LIU Lihua CHEN Yiqiang
The Institute of Respiratory Diseases, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021
关键词:
支气管哮喘高迁移率族蛋白B116HBE血管内皮生长因子
Keywords:
bronchial asthmahighmobility group protein B116HBEvascular endothelial growth factor
分类号:
R562.2
摘要:
摘要目的:探讨高迁移率族蛋白B1(HMGB1)对人支气管上皮细胞(16HBE)血管内皮生长因子(VEGF)表达和分泌的影响及可能调控机制。方法:16HBE按以下方法分组。①HMGB1不同质量浓度组:0、100、500、2 000 μg/L HMGB1刺激16HBE 24 h。②HMGB1不同作用时间组:对照组,2 000 μg/L HMGB1分别刺激16HBE 6、12、24 h组。MTT检测上述各组干预后16HBE的细胞活力,实时荧光定量PCR检测16HBE VEGF mRNA表达水平的变化,ELISA检测细胞培养上清中VEGF水平。应用P38 MAPK通路特异性抑制剂SB202190观察其对HMGB1诱导VEGF表达和分泌的影响。结果:随HMGB1作用浓度的增加和作用时间的延长,VEGF的表达和分泌呈上升趋势(P均<0.05),但细胞活力无明显改变(P均>0.05)。HMGB1刺激增加了16HBE细胞VEGF的表达和分泌,SB202190几乎完全抑制了HMGB1诱导的VEGF的表达和分泌(P均<0.05)。结论:HMGB1可能通过P38 MAPK通路介导支气管上皮细胞VEGF的表达和分泌。
Abstract:
AbstractAim: To investigate the effect of highmobility group protein B1(HMGB1) on the expression and secretion of vascular endothelial growth factor(VEGF) in human bronchial epithelial cell line 16HBE and mechanism underlying this process. Methods: 16HBE cells were allocated into different groups according to the following methods. 1)different concentrations: 16HBE cells were coincubated with different concentrations of HMGB1(0, 100, 500, 2 000 μg/L). 2)different intervention time: 16HBE cells were incubated with HMGB1 at 2 000 μg/L for 6, 12, 24 h, respectively. MTT assay was used to assess the viability of 16HBE cells. The expression of VEGF mRNA was determined by realtime PCR and the level of VEGF in the supernatant was determined by ELISA. The effect of P38 MAPK pathway on HMGBinduced expression and secretion of VEGF was analyzed by use of a special inhibitor,SB202190. Results: The expression and secretion of VEGF had an increasing tendency with the increase of concentration and treatment time of HMGB1(P<0.05), but there was no obvious changes in 16HBE viability. P38 inhibitor SB202190 abolished the HMGB1induced VEGF expression and secretion(P<0.05). Conclusion: HMGB1 increases VEGF expression and secretion via a P38 MAPKdependent pathway in bronchial epithelial cells.

参考文献/References:

参考文献 [1]Lambrecht BN, Hammad H.The airway epithelium in asthma[J].Nat Med, 2012,18(5):684 [2]Lee CG,Link H,Baluk P,et al.Vascular endothelial growth factor (VEGF) induces remodeling and enhances TH2mediated sensitization and inflammation in the lung[J].Nat Med,2004,10(10):1095 [3]Magna M,Pisetsky DS.The role of HMGB1 in the pathogenesis of inflammatory and autoimmune diseases[J].Mol Med,2014,20(20):138 [4]Hou C,Zhao H,Liu L,et al.High mobility group protein B1(HMGB1) in Asthma: comparison of patients with chronic obstructive pulmonary disease and healthy controls[J].Mol Med,2011,17(7/8):807 [5]Shim EJ,Chun E,Lee HS,et al.The role of highmobility group box1 (HMGB1) in the pathogenesis of asthma[J].Clin Exp Allergy,2012,42(6):958 [6]Ullah MA,Loh Z,Gan WJ,et al.Receptor for advanced glycation end products and its ligand highmobility group box1 mediate allergic airway sensitization and airway inflammation[J].J Allergy Clin Immunol,2014,134(2):440 [7]张丹丹,赵海金,周丽芹,等.高迁移率族蛋白B1协同白介素1β可增加人气道上皮细胞白介素8的表达[J].南方医科大学学报,2012,32(12):1764 [8]Lee CG,Ma B,Takyar S,et al.Studies of vascular endothelial growth factor in asthma and chronic obstructive pulmonary disease[J].Proc Am Thorac Soc,2011,8(6):512

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备注/Memo

备注/Memo:
*广西研究生教育创新计划YCBZ2013014 #通信作者,男,1959年7月生,博士,教授,研究方向:细菌生物膜、支气管哮喘、肺栓塞等,E-mail:chenyq163@tom.com
更新日期/Last Update: 1900-01-01