[1]路平),赵潇丹),郝玉娟),等.小鼠骨髓间充质干细胞在子宫内膜损伤小鼠子宫内膜中的定位*[J].郑州大学学报(医学版),2015,(01):101.
 LU Ping),ZHAO Xiaodan),HAO Yujuan),et al.Location of murine bone marrow mesenchymal stem cells in uterine endometrium of murine endometrial damage model[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(01):101.
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小鼠骨髓间充质干细胞在子宫内膜损伤小鼠子宫内膜中的定位*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年01期
页码:
101
栏目:
应用研究
出版日期:
2015-01-20

文章信息/Info

Title:
Location of murine bone marrow mesenchymal stem cells in uterine endometrium of murine endometrial damage model
作者:
路平1)赵潇丹2)郝玉娟2)田捧2)郭玉琪2)#
1)河南省直第三人民医院妇产科 郑州 4500062)郑州大学第三附属医院妇产科 郑州 450052
Author(s):
LU Ping1)ZHAO Xiaodan2)HAO Yujuan2)TIAN Peng2)GUO Yuqi2)
1)Department of Obstetrics and Genecology, Henan Provincial People's Hospital of the Third, Zhengzhou 4500062)Department of Obstetrics and Genecology, the Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
骨髓间充质干细胞子宫内膜移植小鼠
Keywords:
bone marrow mesenchymal stem celluterine endometriumtransplantationmouse
分类号:
Q813.3
摘要:
摘要目的:观察骨髓间充质干细胞(MSCs)在子宫内膜损伤小鼠子宫内膜中的定位,初步探讨MSCs移植用于小鼠子宫内膜损伤后修复的可行性。方法:采用热损伤的方法建立小鼠子宫内膜损伤模型,随机分为MSCs组和对照组,每组15只。将体外分离培养的雄性小鼠MSCs经尾静脉联合宫腔内局部注射植入MSCs组小鼠体内,对照组注射等体积的PBS。通过对移植后子宫内膜组织的SRY DNA原位杂交来定位源自雄性供体的细胞。结果:MSCs组小鼠损伤侧子宫内膜组织中有SRY阳性细胞的定植,对照组小鼠则未见SRY阳性细胞。结论:MSCs可定植于子宫内膜损伤小鼠的子宫内膜组织。
Abstract:
AbstractAim: To study the feasibility of bone marrow mesenchymal stem cells(MSCs) repairing murine uterine endometrial damage. Methods: Mouse uterine endometrial damage models were made by heat injury and randomly allocated into MSCs group and control group, 15 in each group. Bone marrow MSCs were transplanted into MSCs group by intrauterine and intravenous injection. The control group was injected with the same amount of PBS. In situ hybridization was used to observe the expression of SRYpositive cells in the uterine endometrial tissue. Results: It was shown that the MSCs group appeared colonization of the SRYpositive cells in the uterine endometrium tissue, while the control group did not. Conclusion: The bone marrow MSCs could locate in the mouse damaged uterine endometrial tissue.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省计划生育科学技术研究计划项目2012070023;郑州市科技局科技计划基金资助项目112PPTSF3189 #通信作者,女,1964年1月生,博士,主任医师,教授,研究方向:生殖免疫,E-mail: yuqiguo@163.com
更新日期/Last Update: 1900-01-01