[1]王伟△,蔡丽霞,崔志瑞,等.特发性矮小患儿血中p53及p21waf/cip1的表达*[J].郑州大学学报(医学版),2015,(02):191.
 WANG Wei,CAI Lixia,CUI Zhirui,et al.Expressions of p53 and p21waf/cip1 in blood of children with idiopathic short stature[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(02):191.
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特发性矮小患儿血中p53及p21waf/cip1的表达*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年02期
页码:
191
栏目:
论著
出版日期:
2015-03-20

文章信息/Info

Title:
Expressions of p53 and p21waf/cip1 in blood of children with idiopathic short stature
作者:
王伟△蔡丽霞崔志瑞罗向阳
郑州大学第三附属医院儿科 郑州 450052
Author(s):
WANG WeiCAI LixiaCUI ZhiruiLUO Xiangyang
Department of Pediatrics, the Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
p53p21waf/cip1特发性矮小儿童
Keywords:
p53p21waf/cip1idiopathic short staturechildrenAbstract
分类号:
R72
摘要:
目的:观察特发性矮小(ISS)患儿血中p53及细胞周期调节基因p21waf/cip1的表达,探讨二者与ISS发病机制之间的关系。方法:采用实时荧光定量PCR及ELISA法检测30例ISS患儿(ISS组)和30名正常儿童(对照组)外周血中p53和p21waf/cip1 mRNA的表达水平及血清中P53和P21waf/cip1蛋白的含量。结果:ISS组患儿外周血中p53 mRNA的表达水平及血清P53的浓度与对照组相比,差异无统计学意义(t=1.044和0.701,P均>0.05),ISS组p21waf/cip1 mRNA的表达水平较对照组升高(t=4.606,P<0.001)。结论:p21waf/cip1可能通过非依赖p53途径参与ISS的发生。
Abstract:
Aim: To investigate the expressions of p53 and p21waf/cip1 in blood of children with idiopathic short stature(ISS) and to elucidate the pathogenesis of ISS. Methods: Realtime PCR was used to measure the expressions of p53 mRNA and p21waf/cip1 mRNA in peripheral blood from 30 children with ISS(ISS group) and 30 normal control(control group); ELISA was used to measure the levels of P53 and P21waf/cip1 in serum of the children in the 2 groups. Results: The expression of p53 mRNA and the concentration of serum P53 had no statistical significance between the 2 groups(t=1.044 and 0.701,P>0.05). The expression of p21waf/cip1 mRNA in ISS group was significantly higher than those in the control group(t=4.066,P<0.001). Conclusion: p21waf/cip1 may be responsible for the pathogenesis of ISS, which might not depends on p53 pathway.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省医学科技攻关计划项目201303092;河南省卫生科技创新型人才工程专项经费资助豫卫科201052
更新日期/Last Update: 1900-01-01