[1]张香梅△,杨桂林,乐晓华,等.肝细胞癌合并乙型肝炎肝硬化患者癌组织中长链非编码RNA表达谱分析*[J].郑州大学学报(医学版),2015,(02):194.
 ZHANG Xiangmei,YANG Guilin,LE Xiaohua,et al.Expression profiles of long noncoding RNA in HCC patients suffering hepatitis B liver cirrhosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(02):194.
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肝细胞癌合并乙型肝炎肝硬化患者癌组织中长链非编码RNA表达谱分析*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年02期
页码:
194
栏目:
论著
出版日期:
2015-03-20

文章信息/Info

Title:
Expression profiles of long noncoding RNA in HCC patients suffering hepatitis B liver cirrhosis
作者:
张香梅△杨桂林乐晓华李红春陈培芬吴驰苟继周周伯平
深圳市第三人民医院病理科 深圳 518112
Author(s):
ZHANG Xiangmei YANG Guilin LE Xiaohua LI Hongchun CHEN Peifen WU Chi GOU Jizhou ZHOU Boping
Department of Pathology, the Third Peoples Hospital of Shenzhen, Shenzhen 518112
关键词:
乙型肝炎肝硬化肝细胞癌长链非编码RNA
Keywords:
hepatitis B liver cirrhosis hepatocellular carcinoma long noncoding RNA
分类号:
R730.2
摘要:
目的:观察长链非编码RNA(LncRNA)在肝细胞癌及癌旁肝硬化组织中表达谱的差异。方法:运用LncRNA表达谱芯片检测3例肝细胞癌组织及配对癌旁肝硬化组织中的LncRNA,对芯片检测的原始数据进行均一化处理和统计分析,采用分层聚类分析将2组间差异表达的LncRNA进行分类。 从差异表达的LncRNA中选取4个LncRNA在10例中进行qPCR验证,以判断芯片检测结果的可靠性。结果:肝细胞癌组与癌旁肝硬化组2倍以上差异表达的LncRNA共2 225条,其中表达上调的747条(33.6%),表达下调的1 478条(66.4%),5倍以上表达上调的123条,5倍以上表达下调的353条。 qPCR检测结果与芯片检测结果一致。结论:与癌旁肝硬化组织比较,肝细胞癌组织中LncRNA的表达谱具有显著变化。
Abstract:
Aim: To observe the discrepancy of long noncoding RNA(LncRNA) expression profiles between HCC and corresponding adjacent liver tissue. Methods: Utilizing LncRNA hybridizationbased microarrays technology, the LncRNA in 3 cases of HCC tissue and corresponding adjacent liver tissue was detected. Four differentially expressed LncRNA were selected and verified by qPCR in 10 cases of HCC and corresponding adjacent liver cirrhosis tissue.Results: There were 2 225 more than 2fold differentially expressed LncRNA between HCC tissue and corresponding liver cirrhosis tissue: 747 were upregulated, accounting for 33.6%, in which 123 LncRNA upregulated by more than 5 times; 1 478 were downregulated, accounting for 66.4%, in which 353 LncRNA downregulated by more than 5 times. The results of qPCR were consistent with that of microarrays.Conclusion: Compared with adjacent paired liver cirrhosis tissue, there are evidently differentially expressed LncRNA profiles in HCC tissue.

参考文献/References:

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备注/Memo

备注/Memo:
*深圳市科技创新计划项目JCYJ201404111137484100;深圳市科技计划项目(医疗卫生类)201202060
更新日期/Last Update: 1900-01-01