[1]张根豪),刘俊文),苏利沙),等.阿司匹林对人卵巢癌组织和SKOV3细胞SOX7表达和Wnt/βcatenin信号通路的影响*[J].郑州大学学报(医学版),2015,(02):240.
 ZHANG Genhao),LIU Junwen),SU Lisha,et al.Effect of aspirin on expression of SOX7 and Wnt/βcatenin signaling pathway in human ovarian cancer tissue and SKOV3 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(02):240.
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阿司匹林对人卵巢癌组织和SKOV3细胞SOX7表达和Wnt/βcatenin信号通路的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年02期
页码:
240
栏目:
应用研究
出版日期:
2015-03-20

文章信息/Info

Title:
Effect of aspirin on expression of SOX7 and Wnt/βcatenin signaling pathway in human ovarian cancer tissue and SKOV3 cells
作者:
张根豪1)刘俊文1)苏利沙2)刘红春1)#
1)郑州大学第一附属医院检验科 郑州 4500522)郑州大学第一附属医院产前诊断中心 郑州 450052
Author(s):
ZHANG Genhao1)LIU Junwen1)SU Lisha2)LIU Hongchun1)
1)Clinical Laboratory,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 4500522)Center of Prenatal Diagnosis,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
SOX7卵巢癌SKOV3细胞阿司匹林Wnt/βcateninCyclin D1
Keywords:
SOX7ovarian cancerSKOV3 cellaspirinWnt/βcateninCyclin D1
分类号:
R711.75
摘要:
目的:研究阿司匹林对人卵巢癌组织和SKOV3细胞SOX7表达和Wnt/βcatenin信号通路的影响。方法:采用实时荧光定量PCR技术检测20例卵巢癌组织和20例正常卵巢组织中SOX7、βcatenin和Cyclin D1 mRNA的表达;0、0.5、1.0、2.0和3.0 mmol/L阿司匹林体外作用SKOV3细胞48 h后,采用实时荧光定量PCR技术检测细胞SOX7、βcatenin和Cyclin D1 mRNA的表达;MTT法检测0、0.5、1.0、2.0和3.0 mmol/L阿司匹林对SKOV3细胞增殖的影响。结果:与正常卵巢组织相比,卵巢癌组织中SOX7 mRNA表达降低(t=3.614, P=0.001), βcatenin和Cyclin D1 mRNA表达升高(t=13.651和5.967, P<0.001)。0、 0.5、1.0、2.0和3.0 mmol/L阿司匹林作用48 h后,SKOV3细胞中SOX7 mRNA的表达水平逐渐升高(F=12.151,P<0.001),而βcatenin和Cyclin D1 mRNA的表达水平逐渐降低(F=6.214和4.903,P<0.05)。阿司匹林浓度依赖性地抑制SKOV3细胞的增殖(F=20.481,P<0.001)。结论:阿司匹林可能通过上调人卵巢癌中SOX7的表达对Wnt/βcatenin信号通路产生影响。
Abstract:
Aim: To investigate the effect of aspirin on expression of SOX7 and Wnt/βcatenin signaling pathway in human ovarian cancer SKOV3 cells. Methods: The expression levels of SOX7, βcatenin and Cyclin D1 in ovarian tumor tissue and normal ovarian tissue were detected by quantitative realtime PCR(qRTPCR).The SKOV3 cells were stimulated by different concentrations of aspirin in vitro and incubated for 48 h, then the expression of SOX7, βcatenin and Cyclin D1 mRNA were detected by qRTPCR. The effect of aspirin on SKOV3 cell proliferation was measured by MTT assay.Results: The expression of SOX7 mRNA was decreased in ovarian cancer tissue(t=3.614,P=0.001),but the expressions of βcatenin and Cyclin D1 proteins were increased(t=13.651, 5.967, P<0.001). The expression level of SOX7 mRNA was increased with the treatment of increasing concentration of aspirin (F=12.151,P<0.001),but those of βcatenin mRNA and Cyclin D1 mRNA were decreased(F=6.214,4.903,P<0.05) in SKOV3 cell. Aspirin inhibited the cell proliferation of SKOV3 cells in a concerntrationdependent manner(F=20.481,P<0.001).Conclusion: Aspirin may have effect on Wnt/βcatenin signaling pathway by upregulating the expression of SOX7 mRNA in human ovarian cancer SKOV3 cells.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省教育厅重点科技攻关项目2013020036
更新日期/Last Update: 1900-01-01