[1]贾艳艳△,史惠蓉,任芳.子宫内膜异位症患者腹腔液中蛋白质质谱的变化[J].郑州大学学报(医学版),2015,(02):248.
 JIA Yanyan,SHI Huirong,REN Fang.Changes of peritoneal fluid protein mass spectrometry of patients with endometriosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(02):248.
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子宫内膜异位症患者腹腔液中蛋白质质谱的变化
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年02期
页码:
248
栏目:
应用研究
出版日期:
2015-03-20

文章信息/Info

Title:
Changes of peritoneal fluid protein mass spectrometry of patients with endometriosis
作者:
贾艳艳△史惠蓉任芳
郑州大学第一附属医院妇产科 郑州 450052
Author(s):
JIA Yanyan SHI Huirong REN Fang
Department of Gynecology and Obstetrics, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
子宫内膜异位症蛋白质质谱腹腔液
Keywords:
endometriosis protein mass spectrometry peritoneal fluid
分类号:
R711.71
摘要:
目的:了解子宫内膜异位症(简称内异症)患者腹腔液中蛋白质质谱变化。方法:取内异症患者30例、非内异症患者30例的腹腔液,采用SELDITOFMS技术进行蛋白质质谱检测并结合生物信息学方法进行分析。结果:内异症组与非内异症组腹腔液蛋白质质谱间7个蛋白质差异有统计学意义(P均<0.05)。M/Z为9 022.578和6 516.219的2个蛋白质被自动作为分类变量建立决策树分类模型,其敏感性为93.33%(28/30),特异性为83.33%(25/30)。其中M/Z为9 022.578的蛋白质在内异症患者腹腔液中的表达降低,而M/Z为6 516.219的蛋白质在内异症患者腹腔液中的表达升高。结论:从内异症患者腹腔液中筛查出7种差异蛋白质,对进一步研究内异症的病因及发病机制有重要意义。
Abstract:
Aim: To analyse the protein mass spectrometry in the peritoneal fluid of endometriosis patients. Methods: Samples were taken from the peritoneal fluid of patients(30 cases of endometriosis, and 30 cases of nonendometriosis). Protein mass spectrometry were analysed using SELDITOFMS. Results: A total of 7 protein peaks were tested with statistic significance in the peritoneal fluid between the two groups(P<0.05). Two proteins, with M/Z of 9 022.578 and 6 516.219 respectively, were selected for a diagnostic model of endometriosis. The sensitivity and specificity of the model were 93.33%(28/30) and 83.33%(25/30) respectively. The protein with M/Z of 9 022.578 was downregulated, and that with M/Z of 6 516.219 was upregulated in endometriosis patients. Conclusion: The screened 7 protein have important value in further study on etiology and pathogenesis of endometriosis.

参考文献/References:

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更新日期/Last Update: 1900-01-01